OPPORTUNITIES AND CHALLENGE OF SHIGELLOSIS AND SALMONELLOSIS VERIFICATION IN ADULT ACUTE DIARRHEA CASES

The paper suggests the frequency of enteropathogenic agents detected by different lab methods in feces of 262 adult acute diarrhea cases. The compared is the frequency results of Shigella spp. and Salmonella spp.detection by standard methods (culture and specific sera antibody finding) and PCR metho...

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Autores principales: E. A. Kozhukhova, V. D. Ivaschenko
Formato: article
Lenguaje:RU
Publicado: Sankt-Peterburg : NIIÈM imeni Pastera 2015
Materias:
pcr
Acceso en línea:https://doaj.org/article/d06bc880c034403c8acb0f3c18b0588f
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Sumario:The paper suggests the frequency of enteropathogenic agents detected by different lab methods in feces of 262 adult acute diarrhea cases. The compared is the frequency results of Shigella spp. and Salmonella spp.detection by standard methods (culture and specific sera antibody finding) and PCR method. The calculated is some operational characteristics of PCR test (compared to culture test). The shown is that the frequency results of Salmonella spp. detected by standard and PCR methods are comparable, with high figures of all operational characteristics of PCR method. As to Shigella spp. detection the positive result by PCR method were obtained in 2,5 times more cases than that by standard method. However such PCR test characteristic as “positive predictive value” was quite low with the lowest figures in cases without colitis syndrome.