Detection of virulence and specific genes of Salmonella sp. indigenous from Jember, Indonesia

Abstract. Yulian R, Narulita E, Iqbal M, Rochmiyah D, Suryaningsih I, Ningrum DEAF. 2020. Detection of virulence and specific genes of Salmonella sp. indigenous from Jember, Indonesia. Biodiversitas 21: 2889-2892. The bacterium Salmonella sp. is the most common cause of foodborne infection in humans...

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Autores principales:	Ria Yulian, Erlia Narulita, MOCHAMMAD IQBAL, DESY ROCHMIYAH SARI, IRMA SURYANINGSIH, DIAN EKA APRILIA FITRIA NINGRUM
Formato:	article
Lenguaje:	EN
Publicado:	MBI & UNS Solo 2020
Materias:	salmonella, spis, virulence gene, specific gene Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/d06d6d178be14cbea52e6a41f76e2185
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spelling	oai:doaj.org-article:d06d6d178be14cbea52e6a41f76e21852021-11-22T00:40:52ZDetection of virulence and specific genes of Salmonella sp. indigenous from Jember, Indonesia1412-033X2085-472210.13057/biodiv/d210703https://doaj.org/article/d06d6d178be14cbea52e6a41f76e21852020-06-01T00:00:00Zhttps://smujo.id/biodiv/article/view/5634https://doaj.org/toc/1412-033Xhttps://doaj.org/toc/2085-4722Abstract. Yulian R, Narulita E, Iqbal M, Rochmiyah D, Suryaningsih I, Ningrum DEAF. 2020. Detection of virulence and specific genes of Salmonella sp. indigenous from Jember, Indonesia. Biodiversitas 21: 2889-2892. The bacterium Salmonella sp. is the most common cause of foodborne infection in humans. It has the ability to invAde the host, Salmonellosis is highly influenced by the combination of chromosome and plasmid in which Salmonella Pathogenisitas Islands (SPIs) are the biggest gene in the chromosome that are responsible to build specific interaction between Salmonella and the host. This research performed detection on virulence and specific genes in the SPIs area using PCR method for two Salmonella spp. bacterial isolates originated from Jember which were KP2 and P21D. Seven pairs of primers including stn, fimA, spvR (virulence genes), invA, ivaB, spvC, and fliC-d (specific genes) were used. The result revealed that P21D bacteria containing 3 virulence genes i.e. stn, fimA, and spvR. Meanwhile, only two virulent genes stn, and spvR were present in KP2 bacteria. For the detection of specific genes, three genes invA, ivaB, and spvC were present while fliC-d genes were not detected. Only two, invA, and ivaB genes were detected in P21D bacteria, while spvC, and fliC-d were not detected.Ria YulianErlia NarulitaMOCHAMMAD IQBALDESY ROCHMIYAH SARIIRMA SURYANINGSIHDIAN EKA APRILIA FITRIA NINGRUMMBI & UNS Soloarticlesalmonella, spis, virulence gene, specific geneBiology (General)QH301-705.5ENBiodiversitas, Vol 21, Iss 7 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	salmonella, spis, virulence gene, specific gene Biology (General) QH301-705.5
spellingShingle	salmonella, spis, virulence gene, specific gene Biology (General) QH301-705.5 Ria Yulian Erlia Narulita MOCHAMMAD IQBAL DESY ROCHMIYAH SARI IRMA SURYANINGSIH DIAN EKA APRILIA FITRIA NINGRUM Detection of virulence and specific genes of Salmonella sp. indigenous from Jember, Indonesia
description	Abstract. Yulian R, Narulita E, Iqbal M, Rochmiyah D, Suryaningsih I, Ningrum DEAF. 2020. Detection of virulence and specific genes of Salmonella sp. indigenous from Jember, Indonesia. Biodiversitas 21: 2889-2892. The bacterium Salmonella sp. is the most common cause of foodborne infection in humans. It has the ability to invAde the host, Salmonellosis is highly influenced by the combination of chromosome and plasmid in which Salmonella Pathogenisitas Islands (SPIs) are the biggest gene in the chromosome that are responsible to build specific interaction between Salmonella and the host. This research performed detection on virulence and specific genes in the SPIs area using PCR method for two Salmonella spp. bacterial isolates originated from Jember which were KP2 and P21D. Seven pairs of primers including stn, fimA, spvR (virulence genes), invA, ivaB, spvC, and fliC-d (specific genes) were used. The result revealed that P21D bacteria containing 3 virulence genes i.e. stn, fimA, and spvR. Meanwhile, only two virulent genes stn, and spvR were present in KP2 bacteria. For the detection of specific genes, three genes invA, ivaB, and spvC were present while fliC-d genes were not detected. Only two, invA, and ivaB genes were detected in P21D bacteria, while spvC, and fliC-d were not detected.
format	article
author	Ria Yulian Erlia Narulita MOCHAMMAD IQBAL DESY ROCHMIYAH SARI IRMA SURYANINGSIH DIAN EKA APRILIA FITRIA NINGRUM
author_facet	Ria Yulian Erlia Narulita MOCHAMMAD IQBAL DESY ROCHMIYAH SARI IRMA SURYANINGSIH DIAN EKA APRILIA FITRIA NINGRUM
author_sort	Ria Yulian
title	Detection of virulence and specific genes of Salmonella sp. indigenous from Jember, Indonesia
title_short	Detection of virulence and specific genes of Salmonella sp. indigenous from Jember, Indonesia
title_full	Detection of virulence and specific genes of Salmonella sp. indigenous from Jember, Indonesia
title_fullStr	Detection of virulence and specific genes of Salmonella sp. indigenous from Jember, Indonesia
title_full_unstemmed	Detection of virulence and specific genes of Salmonella sp. indigenous from Jember, Indonesia
title_sort	detection of virulence and specific genes of salmonella sp. indigenous from jember, indonesia
publisher	MBI & UNS Solo
publishDate	2020
url	https://doaj.org/article/d06d6d178be14cbea52e6a41f76e2185
work_keys_str_mv	AT riayulian detectionofvirulenceandspecificgenesofsalmonellaspindigenousfromjemberindonesia AT erlianarulita detectionofvirulenceandspecificgenesofsalmonellaspindigenousfromjemberindonesia AT mochammadiqbal detectionofvirulenceandspecificgenesofsalmonellaspindigenousfromjemberindonesia AT desyrochmiyahsari detectionofvirulenceandspecificgenesofsalmonellaspindigenousfromjemberindonesia AT irmasuryaningsih detectionofvirulenceandspecificgenesofsalmonellaspindigenousfromjemberindonesia AT dianekaapriliafitrianingrum detectionofvirulenceandspecificgenesofsalmonellaspindigenousfromjemberindonesia
_version_	1718418550732881920

Detection of virulence and specific genes of Salmonella sp. indigenous from Jember, Indonesia

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