Circulating cell free DNA response to exhaustive exercise in average trained men with type I diabetes mellitus

Abstract It is believed that neutrophils extracellular traps (NETs) formation is responsible for the increase in cf DNA after exercise. Since T1DM is accompanied by enhanced NETs generation, we compared exercise-induced increase in cf DNA in 14 men with T1DM and 11 healthy controls and analyzed its...

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Autores principales:	Konrad Walczak, Robert Stawski, Ewelina Perdas, Olga Brzezinska, Piotr Kosielski, Szymon Galczynski, Tomasz Budlewski, Gianluca Padula, Dariusz Nowak
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/d06f130f80974e44958c3a6388e25aed
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spelling	oai:doaj.org-article:d06f130f80974e44958c3a6388e25aed2021-12-02T15:54:03ZCirculating cell free DNA response to exhaustive exercise in average trained men with type I diabetes mellitus10.1038/s41598-021-84201-02045-2322https://doaj.org/article/d06f130f80974e44958c3a6388e25aed2021-02-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-84201-0https://doaj.org/toc/2045-2322Abstract It is believed that neutrophils extracellular traps (NETs) formation is responsible for the increase in cf DNA after exercise. Since T1DM is accompanied by enhanced NETs generation, we compared exercise-induced increase in cf DNA in 14 men with T1DM and 11 healthy controls and analyzed its association with exercise load. Subjects performed a treadmill run to exhaustion at speed corresponding to 70% of their personal VO2max. Blood was collected before and just after exercise for determination of plasma cf nuclear and mitochondrial DNA (cf n-DNA, cf mt-DNA) by real-time PCR, blood cell count and metabolic markers. Exercise resulted in the increase in median cf n-DNA from 3.9 ng/mL to 21.0 ng/mL in T1DM group and from 3.3 ng/mL to 28.9 ng/mL in controls. Median exercise-induced increment (∆) in cf n-DNA did not differ significantly in both groups (17.8 ng/mL vs. 22.1 ng/mL, p = 0.23), but this variable correlated with run distance (r = 0.66), Δ neutrophils (r = 0.86), Δ creatinine (r = 0.65) and Δ creatine kinase (r = 0.77) only in controls. Pre- and post-exercise cf mt-DNA were not significantly different within and between groups. These suggest low usefulness of Δ cf n-DNA as a marker of exercise intensity in T1DM men.Konrad WalczakRobert StawskiEwelina PerdasOlga BrzezinskaPiotr KosielskiSzymon GalczynskiTomasz BudlewskiGianluca PadulaDariusz NowakNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Konrad Walczak Robert Stawski Ewelina Perdas Olga Brzezinska Piotr Kosielski Szymon Galczynski Tomasz Budlewski Gianluca Padula Dariusz Nowak Circulating cell free DNA response to exhaustive exercise in average trained men with type I diabetes mellitus
description	Abstract It is believed that neutrophils extracellular traps (NETs) formation is responsible for the increase in cf DNA after exercise. Since T1DM is accompanied by enhanced NETs generation, we compared exercise-induced increase in cf DNA in 14 men with T1DM and 11 healthy controls and analyzed its association with exercise load. Subjects performed a treadmill run to exhaustion at speed corresponding to 70% of their personal VO2max. Blood was collected before and just after exercise for determination of plasma cf nuclear and mitochondrial DNA (cf n-DNA, cf mt-DNA) by real-time PCR, blood cell count and metabolic markers. Exercise resulted in the increase in median cf n-DNA from 3.9 ng/mL to 21.0 ng/mL in T1DM group and from 3.3 ng/mL to 28.9 ng/mL in controls. Median exercise-induced increment (∆) in cf n-DNA did not differ significantly in both groups (17.8 ng/mL vs. 22.1 ng/mL, p = 0.23), but this variable correlated with run distance (r = 0.66), Δ neutrophils (r = 0.86), Δ creatinine (r = 0.65) and Δ creatine kinase (r = 0.77) only in controls. Pre- and post-exercise cf mt-DNA were not significantly different within and between groups. These suggest low usefulness of Δ cf n-DNA as a marker of exercise intensity in T1DM men.
format	article
author	Konrad Walczak Robert Stawski Ewelina Perdas Olga Brzezinska Piotr Kosielski Szymon Galczynski Tomasz Budlewski Gianluca Padula Dariusz Nowak
author_facet	Konrad Walczak Robert Stawski Ewelina Perdas Olga Brzezinska Piotr Kosielski Szymon Galczynski Tomasz Budlewski Gianluca Padula Dariusz Nowak
author_sort	Konrad Walczak
title	Circulating cell free DNA response to exhaustive exercise in average trained men with type I diabetes mellitus
title_short	Circulating cell free DNA response to exhaustive exercise in average trained men with type I diabetes mellitus
title_full	Circulating cell free DNA response to exhaustive exercise in average trained men with type I diabetes mellitus
title_fullStr	Circulating cell free DNA response to exhaustive exercise in average trained men with type I diabetes mellitus
title_full_unstemmed	Circulating cell free DNA response to exhaustive exercise in average trained men with type I diabetes mellitus
title_sort	circulating cell free dna response to exhaustive exercise in average trained men with type i diabetes mellitus
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/d06f130f80974e44958c3a6388e25aed
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Circulating cell free DNA response to exhaustive exercise in average trained men with type I diabetes mellitus

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