Prostanoid receptors involved in regulation of the beating rate of neonatal rat cardiomyocytes.

Although prostanoids are known to be involved in regulation of the spontaneous beating rate of cultured neonatal rat cardiomyocytes, the various subtypes of prostanoid receptors have not been investigated in detail. In our experiments, prostaglandin (PG)F(2α) and prostanoid FP receptor agonists (flu...

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Autores principales:	Hakima Mechiche, Stanislas Grassin-Delyle, Arnaud Robinet, Pierre Nazeyrollas, Philippe Devillier
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2012
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/d071c9e1366b466a9d13699e91b62aa2
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spelling	oai:doaj.org-article:d071c9e1366b466a9d13699e91b62aa22021-11-18T07:05:46ZProstanoid receptors involved in regulation of the beating rate of neonatal rat cardiomyocytes.1932-620310.1371/journal.pone.0045273https://doaj.org/article/d071c9e1366b466a9d13699e91b62aa22012-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/22984630/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203Although prostanoids are known to be involved in regulation of the spontaneous beating rate of cultured neonatal rat cardiomyocytes, the various subtypes of prostanoid receptors have not been investigated in detail. In our experiments, prostaglandin (PG)F(2α) and prostanoid FP receptor agonists (fluprostenol, latanoprost and cloprostenol) produced a decrease in the beating rate. Two prostanoid IP receptor agonists (iloprost and beraprost) induced first a marked drop in the beating rate and then definitive abrogation of beating. In contrast, the prostanoid DP receptor agonists (PGD(2) and BW245C) and TP receptor agonists (U-46619) produced increases in the beating rate. Sulprostone (a prostanoid EP(1) and EP(3) receptor agonist) induced marked increases in the beating rate, which were suppressed by SC-19220 (a selective prostanoid EP(1) antagonist). Butaprost (a selective prostanoid EP(2) receptor agonist), misoprostol (a prostanoid EP(2) and EP(3) receptor agonist), 11-deoxy-PGE(1) (a prostanoid EP(2), EP(3) and EP(4) receptor agonist) did not alter the beating rate. Our results strongly suggest that prostanoid EP(1) receptors are involved in positive regulation of the beating rate. Prostanoid EP(1) receptor expression was confirmed by western blotting with a selective antibody. Hence, neonatal rat cardiomyocytes express both prostanoid IP and FP receptors (which negatively regulate the spontaneous beating rate) and prostanoid TP, DP(1) and EP(1) receptors (which positively regulate the spontaneous beating rate).Hakima MechicheStanislas Grassin-DelyleArnaud RobinetPierre NazeyrollasPhilippe DevillierPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 7, Iss 9, p e45273 (2012)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Hakima Mechiche Stanislas Grassin-Delyle Arnaud Robinet Pierre Nazeyrollas Philippe Devillier Prostanoid receptors involved in regulation of the beating rate of neonatal rat cardiomyocytes.
description	Although prostanoids are known to be involved in regulation of the spontaneous beating rate of cultured neonatal rat cardiomyocytes, the various subtypes of prostanoid receptors have not been investigated in detail. In our experiments, prostaglandin (PG)F(2α) and prostanoid FP receptor agonists (fluprostenol, latanoprost and cloprostenol) produced a decrease in the beating rate. Two prostanoid IP receptor agonists (iloprost and beraprost) induced first a marked drop in the beating rate and then definitive abrogation of beating. In contrast, the prostanoid DP receptor agonists (PGD(2) and BW245C) and TP receptor agonists (U-46619) produced increases in the beating rate. Sulprostone (a prostanoid EP(1) and EP(3) receptor agonist) induced marked increases in the beating rate, which were suppressed by SC-19220 (a selective prostanoid EP(1) antagonist). Butaprost (a selective prostanoid EP(2) receptor agonist), misoprostol (a prostanoid EP(2) and EP(3) receptor agonist), 11-deoxy-PGE(1) (a prostanoid EP(2), EP(3) and EP(4) receptor agonist) did not alter the beating rate. Our results strongly suggest that prostanoid EP(1) receptors are involved in positive regulation of the beating rate. Prostanoid EP(1) receptor expression was confirmed by western blotting with a selective antibody. Hence, neonatal rat cardiomyocytes express both prostanoid IP and FP receptors (which negatively regulate the spontaneous beating rate) and prostanoid TP, DP(1) and EP(1) receptors (which positively regulate the spontaneous beating rate).
format	article
author	Hakima Mechiche Stanislas Grassin-Delyle Arnaud Robinet Pierre Nazeyrollas Philippe Devillier
author_facet	Hakima Mechiche Stanislas Grassin-Delyle Arnaud Robinet Pierre Nazeyrollas Philippe Devillier
author_sort	Hakima Mechiche
title	Prostanoid receptors involved in regulation of the beating rate of neonatal rat cardiomyocytes.
title_short	Prostanoid receptors involved in regulation of the beating rate of neonatal rat cardiomyocytes.
title_full	Prostanoid receptors involved in regulation of the beating rate of neonatal rat cardiomyocytes.
title_fullStr	Prostanoid receptors involved in regulation of the beating rate of neonatal rat cardiomyocytes.
title_full_unstemmed	Prostanoid receptors involved in regulation of the beating rate of neonatal rat cardiomyocytes.
title_sort	prostanoid receptors involved in regulation of the beating rate of neonatal rat cardiomyocytes.
publisher	Public Library of Science (PLoS)
publishDate	2012
url	https://doaj.org/article/d071c9e1366b466a9d13699e91b62aa2
work_keys_str_mv	AT hakimamechiche prostanoidreceptorsinvolvedinregulationofthebeatingrateofneonatalratcardiomyocytes AT stanislasgrassindelyle prostanoidreceptorsinvolvedinregulationofthebeatingrateofneonatalratcardiomyocytes AT arnaudrobinet prostanoidreceptorsinvolvedinregulationofthebeatingrateofneonatalratcardiomyocytes AT pierrenazeyrollas prostanoidreceptorsinvolvedinregulationofthebeatingrateofneonatalratcardiomyocytes AT philippedevillier prostanoidreceptorsinvolvedinregulationofthebeatingrateofneonatalratcardiomyocytes
_version_	1718423944302690304

Prostanoid receptors involved in regulation of the beating rate of neonatal rat cardiomyocytes.

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