Near infrared planar tumor imaging and quantification using nanosized Alexa 750-labeled phospholipid micelles

Aristarchos Papagiannaros, Amit Kale, Tatyana S Levchenko, Dmitry Mongayt, William C Hartner, Vladimir P TorchilinDepartment of Pharmaceutical Sciences and Center for Pharmaceutical Biotechnology and Nanomedicine, Northeastern University, Boston, MA, USAAbstract: A novel highly biocompatible near in...

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Autores principales:	Aristarchos Papagiannaros, Amit Kale, Tatyana S Levchenko, Dmitry Mongayt, et al.
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2009
Materias:	Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/d07bff6be9d149b29d858ce897f8e030
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spelling	oai:doaj.org-article:d07bff6be9d149b29d858ce897f8e0302021-12-02T02:10:27ZNear infrared planar tumor imaging and quantification using nanosized Alexa 750-labeled phospholipid micelles1176-91141178-2013https://doaj.org/article/d07bff6be9d149b29d858ce897f8e0302009-05-01T00:00:00Zhttp://www.dovepress.com/near-infrared-planar-tumor-imaging-and-quantification-using-nanosized--a3144https://doaj.org/toc/1176-9114https://doaj.org/toc/1178-2013Aristarchos Papagiannaros, Amit Kale, Tatyana S Levchenko, Dmitry Mongayt, William C Hartner, Vladimir P TorchilinDepartment of Pharmaceutical Sciences and Center for Pharmaceutical Biotechnology and Nanomedicine, Northeastern University, Boston, MA, USAAbstract: A novel highly biocompatible near infrared nanosized contrast agent was developed and used for rapid tumor detection and quantification using planar optical imaging and analysis. With this in mind, the near infrared fluorescent dye Alexa 750 was covalently attached to polyethylene glycol-phosphatidylethanolamine (PEG-PE) conjugate, and double labeled (with Alexa and rhodamine) PEG-PE micelles were injected into mice and observed using planar optical imaging. Pixel intensity data from the tumor site were normalized versus the autofluorescence of the animal at the same time point and normalized as signal to noise over the scattered light from the various tissues of the mice. The detected signal from the tumor was higher than the background noise allowing for rapid detection of the tumor. The tumor was clearly visible within one hour. Some signal was also detected from the abdomen of the mice. As determined by microscopy analysis, other organs of accumulation were the liver and kidney, which corresponded well to the data from the whole body imaging animal studies.Keywords: nanotechnology, nanomedicine, cancer imaging agents, near infrared imaging, micelles Aristarchos PapagiannarosAmit KaleTatyana S LevchenkoDmitry Mongaytet al.Dove Medical PressarticleMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2009, Iss default, Pp 123-131 (2009)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine (General) R5-920
spellingShingle	Medicine (General) R5-920 Aristarchos Papagiannaros Amit Kale Tatyana S Levchenko Dmitry Mongayt et al. Near infrared planar tumor imaging and quantification using nanosized Alexa 750-labeled phospholipid micelles
description	Aristarchos Papagiannaros, Amit Kale, Tatyana S Levchenko, Dmitry Mongayt, William C Hartner, Vladimir P TorchilinDepartment of Pharmaceutical Sciences and Center for Pharmaceutical Biotechnology and Nanomedicine, Northeastern University, Boston, MA, USAAbstract: A novel highly biocompatible near infrared nanosized contrast agent was developed and used for rapid tumor detection and quantification using planar optical imaging and analysis. With this in mind, the near infrared fluorescent dye Alexa 750 was covalently attached to polyethylene glycol-phosphatidylethanolamine (PEG-PE) conjugate, and double labeled (with Alexa and rhodamine) PEG-PE micelles were injected into mice and observed using planar optical imaging. Pixel intensity data from the tumor site were normalized versus the autofluorescence of the animal at the same time point and normalized as signal to noise over the scattered light from the various tissues of the mice. The detected signal from the tumor was higher than the background noise allowing for rapid detection of the tumor. The tumor was clearly visible within one hour. Some signal was also detected from the abdomen of the mice. As determined by microscopy analysis, other organs of accumulation were the liver and kidney, which corresponded well to the data from the whole body imaging animal studies.Keywords: nanotechnology, nanomedicine, cancer imaging agents, near infrared imaging, micelles
format	article
author	Aristarchos Papagiannaros Amit Kale Tatyana S Levchenko Dmitry Mongayt et al.
author_facet	Aristarchos Papagiannaros Amit Kale Tatyana S Levchenko Dmitry Mongayt et al.
author_sort	Aristarchos Papagiannaros
title	Near infrared planar tumor imaging and quantification using nanosized Alexa 750-labeled phospholipid micelles
title_short	Near infrared planar tumor imaging and quantification using nanosized Alexa 750-labeled phospholipid micelles
title_full	Near infrared planar tumor imaging and quantification using nanosized Alexa 750-labeled phospholipid micelles
title_fullStr	Near infrared planar tumor imaging and quantification using nanosized Alexa 750-labeled phospholipid micelles
title_full_unstemmed	Near infrared planar tumor imaging and quantification using nanosized Alexa 750-labeled phospholipid micelles
title_sort	near infrared planar tumor imaging and quantification using nanosized alexa 750-labeled phospholipid micelles
publisher	Dove Medical Press
publishDate	2009
url	https://doaj.org/article/d07bff6be9d149b29d858ce897f8e030
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Near infrared planar tumor imaging and quantification using nanosized Alexa 750-labeled phospholipid micelles

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