Impact of UV sterilization and short term storage on the in vitro release kinetics and bioactivity of biomolecules from electrospun scaffolds

Abstract To effectively translate bioactive scaffolds into a preclinical setting, proper sterilization techniques and storage conditions need to be carefully considered, as the chosen sterilization technique and storage condition might affect the structural and mechanical properties of the scaffolds...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Olivera Evrova, Damian Kellenberger, Chiara Scalera, Maurizio Calcagni, Pietro Giovanoli, Viola Vogel, Johanna Buschmann
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2019
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/d0865ea445ab40f6acbbc4ebe5696013
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:d0865ea445ab40f6acbbc4ebe5696013
record_format	dspace
spelling	oai:doaj.org-article:d0865ea445ab40f6acbbc4ebe56960132021-12-02T16:08:42ZImpact of UV sterilization and short term storage on the in vitro release kinetics and bioactivity of biomolecules from electrospun scaffolds10.1038/s41598-019-51513-12045-2322https://doaj.org/article/d0865ea445ab40f6acbbc4ebe56960132019-10-01T00:00:00Zhttps://doi.org/10.1038/s41598-019-51513-1https://doaj.org/toc/2045-2322Abstract To effectively translate bioactive scaffolds into a preclinical setting, proper sterilization techniques and storage conditions need to be carefully considered, as the chosen sterilization technique and storage condition might affect the structural and mechanical properties of the scaffolds, as well as the bioactivity and release kinetics of the incorporated biomolecules. Since rarely tested or quantified, we show here in a proof-of-concept study how these parameters are affected by UV sterilization and one week storage at different temperatures using bioactive electrospun DegraPol scaffolds that were specifically designed for application in the field of tendon rupture repair. Even though UV sterilization and the different storage conditions did not impact the morphology or the physicochemical properties of the bioactive scaffolds, UV sterilization caused significant attenuation of the growth factor release kinetics, here platelet derived growth factor (PDGF-BB) release (by approx. 85%) and slight decrease in ascorbic acid release (by approx. 20%). In contrast, 4 °C and −20 °C storage did not have a major effect on the release kinetics of PDGF-BB, while storage at room temperature caused increase in PDGF-BB released. All storage conditions had little effect on ascorbic acid release. Equally important, neither UV sterilization nor storage affected the bioactivity of the released PDGF-BB, suggesting stability of the bioactive scaffolds for at least one week and showing potential for bioactive DegraPol scaffolds to be translated into an off-the-shelf available product. These parameters are expected to be scaffold and protein-dependent.Olivera EvrovaDamian KellenbergerChiara ScaleraMaurizio CalcagniPietro GiovanoliViola VogelJohanna BuschmannNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 9, Iss 1, Pp 1-11 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Olivera Evrova Damian Kellenberger Chiara Scalera Maurizio Calcagni Pietro Giovanoli Viola Vogel Johanna Buschmann Impact of UV sterilization and short term storage on the in vitro release kinetics and bioactivity of biomolecules from electrospun scaffolds
description	Abstract To effectively translate bioactive scaffolds into a preclinical setting, proper sterilization techniques and storage conditions need to be carefully considered, as the chosen sterilization technique and storage condition might affect the structural and mechanical properties of the scaffolds, as well as the bioactivity and release kinetics of the incorporated biomolecules. Since rarely tested or quantified, we show here in a proof-of-concept study how these parameters are affected by UV sterilization and one week storage at different temperatures using bioactive electrospun DegraPol scaffolds that were specifically designed for application in the field of tendon rupture repair. Even though UV sterilization and the different storage conditions did not impact the morphology or the physicochemical properties of the bioactive scaffolds, UV sterilization caused significant attenuation of the growth factor release kinetics, here platelet derived growth factor (PDGF-BB) release (by approx. 85%) and slight decrease in ascorbic acid release (by approx. 20%). In contrast, 4 °C and −20 °C storage did not have a major effect on the release kinetics of PDGF-BB, while storage at room temperature caused increase in PDGF-BB released. All storage conditions had little effect on ascorbic acid release. Equally important, neither UV sterilization nor storage affected the bioactivity of the released PDGF-BB, suggesting stability of the bioactive scaffolds for at least one week and showing potential for bioactive DegraPol scaffolds to be translated into an off-the-shelf available product. These parameters are expected to be scaffold and protein-dependent.
format	article
author	Olivera Evrova Damian Kellenberger Chiara Scalera Maurizio Calcagni Pietro Giovanoli Viola Vogel Johanna Buschmann
author_facet	Olivera Evrova Damian Kellenberger Chiara Scalera Maurizio Calcagni Pietro Giovanoli Viola Vogel Johanna Buschmann
author_sort	Olivera Evrova
title	Impact of UV sterilization and short term storage on the in vitro release kinetics and bioactivity of biomolecules from electrospun scaffolds
title_short	Impact of UV sterilization and short term storage on the in vitro release kinetics and bioactivity of biomolecules from electrospun scaffolds
title_full	Impact of UV sterilization and short term storage on the in vitro release kinetics and bioactivity of biomolecules from electrospun scaffolds
title_fullStr	Impact of UV sterilization and short term storage on the in vitro release kinetics and bioactivity of biomolecules from electrospun scaffolds
title_full_unstemmed	Impact of UV sterilization and short term storage on the in vitro release kinetics and bioactivity of biomolecules from electrospun scaffolds
title_sort	impact of uv sterilization and short term storage on the in vitro release kinetics and bioactivity of biomolecules from electrospun scaffolds
publisher	Nature Portfolio
publishDate	2019
url	https://doaj.org/article/d0865ea445ab40f6acbbc4ebe5696013
work_keys_str_mv	AT oliveraevrova impactofuvsterilizationandshorttermstorageontheinvitroreleasekineticsandbioactivityofbiomoleculesfromelectrospunscaffolds AT damiankellenberger impactofuvsterilizationandshorttermstorageontheinvitroreleasekineticsandbioactivityofbiomoleculesfromelectrospunscaffolds AT chiarascalera impactofuvsterilizationandshorttermstorageontheinvitroreleasekineticsandbioactivityofbiomoleculesfromelectrospunscaffolds AT mauriziocalcagni impactofuvsterilizationandshorttermstorageontheinvitroreleasekineticsandbioactivityofbiomoleculesfromelectrospunscaffolds AT pietrogiovanoli impactofuvsterilizationandshorttermstorageontheinvitroreleasekineticsandbioactivityofbiomoleculesfromelectrospunscaffolds AT violavogel impactofuvsterilizationandshorttermstorageontheinvitroreleasekineticsandbioactivityofbiomoleculesfromelectrospunscaffolds AT johannabuschmann impactofuvsterilizationandshorttermstorageontheinvitroreleasekineticsandbioactivityofbiomoleculesfromelectrospunscaffolds
_version_	1718384497549901824

Impact of UV sterilization and short term storage on the in vitro release kinetics and bioactivity of biomolecules from electrospun scaffolds

Ejemplares similares