Development of molecular clamp stabilized hemagglutinin vaccines for Influenza A viruses

Abstract Influenza viruses cause a significant number of infections and deaths annually. In addition to seasonal infections, the risk of an influenza virus pandemic emerging is extremely high owing to the large reservoir of diverse influenza viruses found in animals and the co-circulation of many in...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Christopher L. D. McMillan, Stacey T. M. Cheung, Naphak Modhiran, James Barnes, Alberto A. Amarilla, Helle Bielefeldt-Ohmann, Leo Yi Yang Lee, Kate Guilfoyle, Geert van Amerongen, Koert Stittelaar, Virginie Jakon, Celia Lebas, Patrick Reading, Kirsty R. Short, Paul R. Young, Daniel Watterson, Keith J. Chappell
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Immunologic diseases. Allergy RC581-607 Neoplasms. Tumors. Oncology. Including cancer and carcinogens RC254-282
Acceso en línea:	https://doaj.org/article/d088126b54144c22b6c71abe6699d605
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:d088126b54144c22b6c71abe6699d605
record_format	dspace
spelling	oai:doaj.org-article:d088126b54144c22b6c71abe6699d6052021-11-14T12:07:48ZDevelopment of molecular clamp stabilized hemagglutinin vaccines for Influenza A viruses10.1038/s41541-021-00395-42059-0105https://doaj.org/article/d088126b54144c22b6c71abe6699d6052021-11-01T00:00:00Zhttps://doi.org/10.1038/s41541-021-00395-4https://doaj.org/toc/2059-0105Abstract Influenza viruses cause a significant number of infections and deaths annually. In addition to seasonal infections, the risk of an influenza virus pandemic emerging is extremely high owing to the large reservoir of diverse influenza viruses found in animals and the co-circulation of many influenza subtypes which can reassort into novel strains. Development of a universal influenza vaccine has proven extremely challenging. In the absence of such a vaccine, rapid response technologies provide the best potential to counter a novel influenza outbreak. Here, we demonstrate that a modular trimerization domain known as the molecular clamp allows the efficient production and purification of conformationally stabilised prefusion hemagglutinin (HA) from a diverse range of influenza A subtypes. These clamp-stabilised HA proteins provided robust protection from homologous virus challenge in mouse and ferret models and some cross protection against heterologous virus challenge. This work provides a proof-of-concept for clamp-stabilised HA vaccines as a tool for rapid response vaccine development against future influenza A virus pandemics.Christopher L. D. McMillanStacey T. M. CheungNaphak ModhiranJames BarnesAlberto A. AmarillaHelle Bielefeldt-OhmannLeo Yi Yang LeeKate GuilfoyleGeert van AmerongenKoert StittelaarVirginie JakonCelia LebasPatrick ReadingKirsty R. ShortPaul R. YoungDaniel WattersonKeith J. ChappellNature PortfolioarticleImmunologic diseases. AllergyRC581-607Neoplasms. Tumors. Oncology. Including cancer and carcinogensRC254-282ENnpj Vaccines, Vol 6, Iss 1, Pp 1-12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Immunologic diseases. Allergy RC581-607 Neoplasms. Tumors. Oncology. Including cancer and carcinogens RC254-282
spellingShingle	Immunologic diseases. Allergy RC581-607 Neoplasms. Tumors. Oncology. Including cancer and carcinogens RC254-282 Christopher L. D. McMillan Stacey T. M. Cheung Naphak Modhiran James Barnes Alberto A. Amarilla Helle Bielefeldt-Ohmann Leo Yi Yang Lee Kate Guilfoyle Geert van Amerongen Koert Stittelaar Virginie Jakon Celia Lebas Patrick Reading Kirsty R. Short Paul R. Young Daniel Watterson Keith J. Chappell Development of molecular clamp stabilized hemagglutinin vaccines for Influenza A viruses
description	Abstract Influenza viruses cause a significant number of infections and deaths annually. In addition to seasonal infections, the risk of an influenza virus pandemic emerging is extremely high owing to the large reservoir of diverse influenza viruses found in animals and the co-circulation of many influenza subtypes which can reassort into novel strains. Development of a universal influenza vaccine has proven extremely challenging. In the absence of such a vaccine, rapid response technologies provide the best potential to counter a novel influenza outbreak. Here, we demonstrate that a modular trimerization domain known as the molecular clamp allows the efficient production and purification of conformationally stabilised prefusion hemagglutinin (HA) from a diverse range of influenza A subtypes. These clamp-stabilised HA proteins provided robust protection from homologous virus challenge in mouse and ferret models and some cross protection against heterologous virus challenge. This work provides a proof-of-concept for clamp-stabilised HA vaccines as a tool for rapid response vaccine development against future influenza A virus pandemics.
format	article
author	Christopher L. D. McMillan Stacey T. M. Cheung Naphak Modhiran James Barnes Alberto A. Amarilla Helle Bielefeldt-Ohmann Leo Yi Yang Lee Kate Guilfoyle Geert van Amerongen Koert Stittelaar Virginie Jakon Celia Lebas Patrick Reading Kirsty R. Short Paul R. Young Daniel Watterson Keith J. Chappell
author_facet	Christopher L. D. McMillan Stacey T. M. Cheung Naphak Modhiran James Barnes Alberto A. Amarilla Helle Bielefeldt-Ohmann Leo Yi Yang Lee Kate Guilfoyle Geert van Amerongen Koert Stittelaar Virginie Jakon Celia Lebas Patrick Reading Kirsty R. Short Paul R. Young Daniel Watterson Keith J. Chappell
author_sort	Christopher L. D. McMillan
title	Development of molecular clamp stabilized hemagglutinin vaccines for Influenza A viruses
title_short	Development of molecular clamp stabilized hemagglutinin vaccines for Influenza A viruses
title_full	Development of molecular clamp stabilized hemagglutinin vaccines for Influenza A viruses
title_fullStr	Development of molecular clamp stabilized hemagglutinin vaccines for Influenza A viruses
title_full_unstemmed	Development of molecular clamp stabilized hemagglutinin vaccines for Influenza A viruses
title_sort	development of molecular clamp stabilized hemagglutinin vaccines for influenza a viruses
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/d088126b54144c22b6c71abe6699d605
work_keys_str_mv	AT christopherldmcmillan developmentofmolecularclampstabilizedhemagglutininvaccinesforinfluenzaaviruses AT staceytmcheung developmentofmolecularclampstabilizedhemagglutininvaccinesforinfluenzaaviruses AT naphakmodhiran developmentofmolecularclampstabilizedhemagglutininvaccinesforinfluenzaaviruses AT jamesbarnes developmentofmolecularclampstabilizedhemagglutininvaccinesforinfluenzaaviruses AT albertoaamarilla developmentofmolecularclampstabilizedhemagglutininvaccinesforinfluenzaaviruses AT hellebielefeldtohmann developmentofmolecularclampstabilizedhemagglutininvaccinesforinfluenzaaviruses AT leoyiyanglee developmentofmolecularclampstabilizedhemagglutininvaccinesforinfluenzaaviruses AT kateguilfoyle developmentofmolecularclampstabilizedhemagglutininvaccinesforinfluenzaaviruses AT geertvanamerongen developmentofmolecularclampstabilizedhemagglutininvaccinesforinfluenzaaviruses AT koertstittelaar developmentofmolecularclampstabilizedhemagglutininvaccinesforinfluenzaaviruses AT virginiejakon developmentofmolecularclampstabilizedhemagglutininvaccinesforinfluenzaaviruses AT celialebas developmentofmolecularclampstabilizedhemagglutininvaccinesforinfluenzaaviruses AT patrickreading developmentofmolecularclampstabilizedhemagglutininvaccinesforinfluenzaaviruses AT kirstyrshort developmentofmolecularclampstabilizedhemagglutininvaccinesforinfluenzaaviruses AT paulryoung developmentofmolecularclampstabilizedhemagglutininvaccinesforinfluenzaaviruses AT danielwatterson developmentofmolecularclampstabilizedhemagglutininvaccinesforinfluenzaaviruses AT keithjchappell developmentofmolecularclampstabilizedhemagglutininvaccinesforinfluenzaaviruses
_version_	1718429432829444096

Development of molecular clamp stabilized hemagglutinin vaccines for Influenza A viruses

Ejemplares similares