Stress activated protein kinase pathway modulates homologous recombination in fission yeast.

Rad52 is a key player in homologous recombination (HR), a DNA repair pathway that is dedicated to double strand breaks repair and recovery of perturbed replication forks. Here we show that fission yeast Rad52 homologue is phosphorylated when S phase cells are exposed to ROS inducers such as ultravio...

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Autores principales:	Angela Bellini, Pierre-Marie Girard, Sarah Lambert, Ludovic Tessier, Evelyne Sage, Stefania Francesconi
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2012
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/d08acc6741f248d3bfae9a21fff4ad15
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spelling	oai:doaj.org-article:d08acc6741f248d3bfae9a21fff4ad152021-11-18T08:10:30ZStress activated protein kinase pathway modulates homologous recombination in fission yeast.1932-620310.1371/journal.pone.0047987https://doaj.org/article/d08acc6741f248d3bfae9a21fff4ad152012-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23118915/?tool=EBIhttps://doaj.org/toc/1932-6203Rad52 is a key player in homologous recombination (HR), a DNA repair pathway that is dedicated to double strand breaks repair and recovery of perturbed replication forks. Here we show that fission yeast Rad52 homologue is phosphorylated when S phase cells are exposed to ROS inducers such as ultraviolet A radiation or hydrogen peroxide, but not to ultraviolet C or camptothecin. Phosphorylation does not depend on kinases Chk1, Rad3, Tel1 or Cdc2, but depends on a functional stress activated protein kinase (SAPK) pathway and can be partially prevented by anti-oxidant treatment. Indeed, cells lacking Sty1, the major fission yeast MAP kinase of the SAPK pathway, do not display Rad52 phosphorylation and have UVA induced Rad52 foci that persist longer if compared to wild type cells. In addition, spontaneous intrachromosomal HR is diminished in cells lacking Sty1 and, more precisely, gene conversion is affected. Moreover, HR induced by site-specific arrest of replication forks is twice less efficient in cells that do not express Sty1. Importantly, impairing HR by deletion of the gene encoding the recombinase Rhp51 leads to Sty1 dependent Rad52 phosphorylation. Thus, SAPK pathway impinges on early step of HR through phosphorylation of Rad52 in cells challenged by oxidative stress or lacking Rhp51 and is required to promote spontaneous gene conversion and recovery from blocked replication forks.Angela BelliniPierre-Marie GirardSarah LambertLudovic TessierEvelyne SageStefania FrancesconiPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 7, Iss 10, p e47987 (2012)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Angela Bellini Pierre-Marie Girard Sarah Lambert Ludovic Tessier Evelyne Sage Stefania Francesconi Stress activated protein kinase pathway modulates homologous recombination in fission yeast.
description	Rad52 is a key player in homologous recombination (HR), a DNA repair pathway that is dedicated to double strand breaks repair and recovery of perturbed replication forks. Here we show that fission yeast Rad52 homologue is phosphorylated when S phase cells are exposed to ROS inducers such as ultraviolet A radiation or hydrogen peroxide, but not to ultraviolet C or camptothecin. Phosphorylation does not depend on kinases Chk1, Rad3, Tel1 or Cdc2, but depends on a functional stress activated protein kinase (SAPK) pathway and can be partially prevented by anti-oxidant treatment. Indeed, cells lacking Sty1, the major fission yeast MAP kinase of the SAPK pathway, do not display Rad52 phosphorylation and have UVA induced Rad52 foci that persist longer if compared to wild type cells. In addition, spontaneous intrachromosomal HR is diminished in cells lacking Sty1 and, more precisely, gene conversion is affected. Moreover, HR induced by site-specific arrest of replication forks is twice less efficient in cells that do not express Sty1. Importantly, impairing HR by deletion of the gene encoding the recombinase Rhp51 leads to Sty1 dependent Rad52 phosphorylation. Thus, SAPK pathway impinges on early step of HR through phosphorylation of Rad52 in cells challenged by oxidative stress or lacking Rhp51 and is required to promote spontaneous gene conversion and recovery from blocked replication forks.
format	article
author	Angela Bellini Pierre-Marie Girard Sarah Lambert Ludovic Tessier Evelyne Sage Stefania Francesconi
author_facet	Angela Bellini Pierre-Marie Girard Sarah Lambert Ludovic Tessier Evelyne Sage Stefania Francesconi
author_sort	Angela Bellini
title	Stress activated protein kinase pathway modulates homologous recombination in fission yeast.
title_short	Stress activated protein kinase pathway modulates homologous recombination in fission yeast.
title_full	Stress activated protein kinase pathway modulates homologous recombination in fission yeast.
title_fullStr	Stress activated protein kinase pathway modulates homologous recombination in fission yeast.
title_full_unstemmed	Stress activated protein kinase pathway modulates homologous recombination in fission yeast.
title_sort	stress activated protein kinase pathway modulates homologous recombination in fission yeast.
publisher	Public Library of Science (PLoS)
publishDate	2012
url	https://doaj.org/article/d08acc6741f248d3bfae9a21fff4ad15
work_keys_str_mv	AT angelabellini stressactivatedproteinkinasepathwaymodulateshomologousrecombinationinfissionyeast AT pierremariegirard stressactivatedproteinkinasepathwaymodulateshomologousrecombinationinfissionyeast AT sarahlambert stressactivatedproteinkinasepathwaymodulateshomologousrecombinationinfissionyeast AT ludovictessier stressactivatedproteinkinasepathwaymodulateshomologousrecombinationinfissionyeast AT evelynesage stressactivatedproteinkinasepathwaymodulateshomologousrecombinationinfissionyeast AT stefaniafrancesconi stressactivatedproteinkinasepathwaymodulateshomologousrecombinationinfissionyeast
_version_	1718422136902647808

Stress activated protein kinase pathway modulates homologous recombination in fission yeast.

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