Seroprevalence and Molecular Detection of Foot and Mouth Disease Virus in Dairy Cattle Around Addis Ababa, Central Ethiopia

Shazali Mohammed Awel,1,2 Getachew Mulatu Dilba,3 Bruk Abraha,2 Demeke Zewde,4 Bayeta Senbata Wakjira,4 Abde Aliy4 1Jigjiga University, College of Veterinary Medicine, Jigjiga, Ethiopia; 2Haramaya University, College of Veterinary Medicine, Dire Dawa, Ethiopia; 3Ambo University, College of Agricultu...

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Autores principales: Awel SM, Dilba GM, Abraha B, Zewde D, Wakjira BS, Aliy A
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Publicado: Dove Medical Press 2021
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spelling oai:doaj.org-article:d08eb9a2fa3846768d857188084d41f92021-12-02T16:08:51ZSeroprevalence and Molecular Detection of Foot and Mouth Disease Virus in Dairy Cattle Around Addis Ababa, Central Ethiopia2230-2034https://doaj.org/article/d08eb9a2fa3846768d857188084d41f92021-07-01T00:00:00Zhttps://www.dovepress.com/seroprevalence-and-molecular-detection-of-foot-and-mouth-disease-virus-peer-reviewed-fulltext-article-VMRRhttps://doaj.org/toc/2230-2034Shazali Mohammed Awel,1,2 Getachew Mulatu Dilba,3 Bruk Abraha,2 Demeke Zewde,4 Bayeta Senbata Wakjira,4 Abde Aliy4 1Jigjiga University, College of Veterinary Medicine, Jigjiga, Ethiopia; 2Haramaya University, College of Veterinary Medicine, Dire Dawa, Ethiopia; 3Ambo University, College of Agriculture and Veterinary Science, Department of Veterinary Laboratory Technology, Ambo, Oromia, Ethiopia; 4National Animal Health Diagnostic and Investigation Center, Sebeta, EthiopiaCorrespondence: Getachew Mulatu DilbaAmbo University, College of Agriculture and Veterinary Science, Department of Veterinary Laboratory Technology, P.O. Box:19, Ambo, Oromia, EthiopiaTel +251-912717217Email getachew.mulatu@ambou.edu.etBackground: Foot and mouth disease is a debilitating and highly contagious transboundary disease of cattle that can cause a huge economical loss globally. It is a notifiable disease in Ethiopia, and it is thought to be causing a decrease in cattle productivity and production.Methods: A cross-sectional study and outbreak investigation were performed to estimate seroprevalence, identify associated factors and serotypes of FMDV in dairy cattle around Addis Ababa. A multi-stage random sampling technique was employed for the selection of sampling units for the seroprevalence study. A total of 383 blood samples were collected using plain vacutainer tubes and the obtained sera were tested by 3ABC-Ab ELISA at the NAHDIC lab. Also, from outbreak cases, 20 epithelial tissue samples were collected purposively for the molecular detection of FMDV serotypes.Results: The overall seroprevalence of FMD in dairy cattle was 72.1% (95% CI=67.27– 76.50). The seroprevalence in dairy cattle of Ada Berga, Holeta, and Sululta districts was 97.2%, 71.4%, and 57.6%, respectively. Up on Chi-square analysis, age, body condition, and management system were significantly associated with FMD seroprevalence (p< 0.05). Besides, multivariable logistic regression analysis showed that district, age, body condition, and management were significantly associated with FMD seroprevalence (p< 0.05). The odds of being seropositive to FMD were 6.9 (95% CI=1.8– 24.9; p=0.005) and 2.3 (95% CI=1.2– 4.7; p=0.01) times higher in cattle found in Ada Berga and Holeta Woreda. From outbreak cases, 18 (90.0%) were identified positive for FMDV serotype O.Conclusion: The current study revealed higher seroprevalence was recorded in the study area and associated risk factors identified statically, serotype O of FMDV was identified from outbreak cases. Therefore, it is critical to design and implement feasible control and prevention mechanisms based on the type of circulating virus serotype.Keywords: central Ethiopia, dairy cattle, FMD virus, molecular detection, seroprevalenceAwel SMDilba GMAbraha BZewde DWakjira BSAliy ADove Medical Pressarticlecentral ethiopiadairy cattlefmd virusmolecular detectionseroprevalenceVeterinary medicineSF600-1100ENVeterinary Medicine: Research and Reports, Vol Volume 12, Pp 187-197 (2021)
institution DOAJ
collection DOAJ
language EN
topic central ethiopia
dairy cattle
fmd virus
molecular detection
seroprevalence
Veterinary medicine
SF600-1100
spellingShingle central ethiopia
dairy cattle
fmd virus
molecular detection
seroprevalence
Veterinary medicine
SF600-1100
Awel SM
Dilba GM
Abraha B
Zewde D
Wakjira BS
Aliy A
Seroprevalence and Molecular Detection of Foot and Mouth Disease Virus in Dairy Cattle Around Addis Ababa, Central Ethiopia
description Shazali Mohammed Awel,1,2 Getachew Mulatu Dilba,3 Bruk Abraha,2 Demeke Zewde,4 Bayeta Senbata Wakjira,4 Abde Aliy4 1Jigjiga University, College of Veterinary Medicine, Jigjiga, Ethiopia; 2Haramaya University, College of Veterinary Medicine, Dire Dawa, Ethiopia; 3Ambo University, College of Agriculture and Veterinary Science, Department of Veterinary Laboratory Technology, Ambo, Oromia, Ethiopia; 4National Animal Health Diagnostic and Investigation Center, Sebeta, EthiopiaCorrespondence: Getachew Mulatu DilbaAmbo University, College of Agriculture and Veterinary Science, Department of Veterinary Laboratory Technology, P.O. Box:19, Ambo, Oromia, EthiopiaTel +251-912717217Email getachew.mulatu@ambou.edu.etBackground: Foot and mouth disease is a debilitating and highly contagious transboundary disease of cattle that can cause a huge economical loss globally. It is a notifiable disease in Ethiopia, and it is thought to be causing a decrease in cattle productivity and production.Methods: A cross-sectional study and outbreak investigation were performed to estimate seroprevalence, identify associated factors and serotypes of FMDV in dairy cattle around Addis Ababa. A multi-stage random sampling technique was employed for the selection of sampling units for the seroprevalence study. A total of 383 blood samples were collected using plain vacutainer tubes and the obtained sera were tested by 3ABC-Ab ELISA at the NAHDIC lab. Also, from outbreak cases, 20 epithelial tissue samples were collected purposively for the molecular detection of FMDV serotypes.Results: The overall seroprevalence of FMD in dairy cattle was 72.1% (95% CI=67.27– 76.50). The seroprevalence in dairy cattle of Ada Berga, Holeta, and Sululta districts was 97.2%, 71.4%, and 57.6%, respectively. Up on Chi-square analysis, age, body condition, and management system were significantly associated with FMD seroprevalence (p< 0.05). Besides, multivariable logistic regression analysis showed that district, age, body condition, and management were significantly associated with FMD seroprevalence (p< 0.05). The odds of being seropositive to FMD were 6.9 (95% CI=1.8– 24.9; p=0.005) and 2.3 (95% CI=1.2– 4.7; p=0.01) times higher in cattle found in Ada Berga and Holeta Woreda. From outbreak cases, 18 (90.0%) were identified positive for FMDV serotype O.Conclusion: The current study revealed higher seroprevalence was recorded in the study area and associated risk factors identified statically, serotype O of FMDV was identified from outbreak cases. Therefore, it is critical to design and implement feasible control and prevention mechanisms based on the type of circulating virus serotype.Keywords: central Ethiopia, dairy cattle, FMD virus, molecular detection, seroprevalence
format article
author Awel SM
Dilba GM
Abraha B
Zewde D
Wakjira BS
Aliy A
author_facet Awel SM
Dilba GM
Abraha B
Zewde D
Wakjira BS
Aliy A
author_sort Awel SM
title Seroprevalence and Molecular Detection of Foot and Mouth Disease Virus in Dairy Cattle Around Addis Ababa, Central Ethiopia
title_short Seroprevalence and Molecular Detection of Foot and Mouth Disease Virus in Dairy Cattle Around Addis Ababa, Central Ethiopia
title_full Seroprevalence and Molecular Detection of Foot and Mouth Disease Virus in Dairy Cattle Around Addis Ababa, Central Ethiopia
title_fullStr Seroprevalence and Molecular Detection of Foot and Mouth Disease Virus in Dairy Cattle Around Addis Ababa, Central Ethiopia
title_full_unstemmed Seroprevalence and Molecular Detection of Foot and Mouth Disease Virus in Dairy Cattle Around Addis Ababa, Central Ethiopia
title_sort seroprevalence and molecular detection of foot and mouth disease virus in dairy cattle around addis ababa, central ethiopia
publisher Dove Medical Press
publishDate 2021
url https://doaj.org/article/d08eb9a2fa3846768d857188084d41f9
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