Association between adiponectin rs17300539 and rs266729 gene polymorphisms with serum adiponectin level in an Iranian diabetic/pre-diabetic population

Objective. Adiponectin is an adipokine that is mostly secreted from adipose tissues and has a significant role in the improvement of insulin resistant and type 2 diabetes mellitus (T2DM). This study is conducted to examine the association of rs17300539 and rs266729 with T2DM and serum adiponectin le...

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Autores principales:	Karimi Helma, Nezhadali Masoumeh, Hedayati Mehdi
Formato:	article
Lenguaje:	EN
Publicado:	Sciendo 2018
Materias:	diabetes mellitus type 2 obesity polymorphism adipose tissue rs17300539 Diseases of the endocrine glands. Clinical endocrinology RC648-665
Acceso en línea:	https://doaj.org/article/d09009732f424ee79fbad37e5b655126
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spelling	oai:doaj.org-article:d09009732f424ee79fbad37e5b6551262021-12-02T16:41:13ZAssociation between adiponectin rs17300539 and rs266729 gene polymorphisms with serum adiponectin level in an Iranian diabetic/pre-diabetic population1336-032910.2478/enr-2018-0022https://doaj.org/article/d09009732f424ee79fbad37e5b6551262018-10-01T00:00:00Zhttps://doi.org/10.2478/enr-2018-0022https://doaj.org/toc/1336-0329Objective. Adiponectin is an adipokine that is mostly secreted from adipose tissues and has a significant role in the improvement of insulin resistant and type 2 diabetes mellitus (T2DM). This study is conducted to examine the association of rs17300539 and rs266729 with T2DM and serum adiponectin level in Iranian population. Method. A case-control study was conducted on 80 individuals with fasting plasma glucose (FPG) >100 (mg/dl) as diabetic-pre-diabetic group, and 80 individuals with fasting plasma glucose 70-100 (mg/dl) as control group. DNA extraction was done on samples and genotyping method was performed by PCR-RFLP. Result. The frequency of GA genotype in rs17300539 (diabetic/pre-diabetic 35.5%, control 11.3%, (OR [95%CI]=4.18[1.8-9.6]; p=0.001) and allele A (diabetic/pre-diabetic 31%, control 9%, (OR [95% CI]=4.67[2-10.7]) was significantly more in diabetic/pre-diabetic group compared to control group. The difference in the genotype frequency for rs266729 in diabetic group compared to that in control was not significant. The levels of adiponectin in diabetic cases had no difference compared to the control group in both polymorphisms. The rs266729 was not associated with any metabolic parameter except waist circumference (p=0.03), however, rs17300539 shows association only with fasting plasma glucose, triglyceride, and total cholesterol (p=0.007, 0.039, 0.0032, respectively). Conclusion. Our findings showed that there is an association between rs17300539 with the increase of T2DM but rs266729 showed no association with the risk of T2DM. Allele A of rs17300539 increased the risk of diabetes. There is no association between adiponectin level and both polymorphisms.Karimi HelmaNezhadali MasoumehHedayati MehdiSciendoarticlediabetes mellitus type 2obesitypolymorphismadipose tissuers17300539Diseases of the endocrine glands. Clinical endocrinologyRC648-665ENEndocrine Regulations, Vol 52, Iss 4, Pp 176-184 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	diabetes mellitus type 2 obesity polymorphism adipose tissue rs17300539 Diseases of the endocrine glands. Clinical endocrinology RC648-665
spellingShingle	diabetes mellitus type 2 obesity polymorphism adipose tissue rs17300539 Diseases of the endocrine glands. Clinical endocrinology RC648-665 Karimi Helma Nezhadali Masoumeh Hedayati Mehdi Association between adiponectin rs17300539 and rs266729 gene polymorphisms with serum adiponectin level in an Iranian diabetic/pre-diabetic population
description	Objective. Adiponectin is an adipokine that is mostly secreted from adipose tissues and has a significant role in the improvement of insulin resistant and type 2 diabetes mellitus (T2DM). This study is conducted to examine the association of rs17300539 and rs266729 with T2DM and serum adiponectin level in Iranian population. Method. A case-control study was conducted on 80 individuals with fasting plasma glucose (FPG) >100 (mg/dl) as diabetic-pre-diabetic group, and 80 individuals with fasting plasma glucose 70-100 (mg/dl) as control group. DNA extraction was done on samples and genotyping method was performed by PCR-RFLP. Result. The frequency of GA genotype in rs17300539 (diabetic/pre-diabetic 35.5%, control 11.3%, (OR [95%CI]=4.18[1.8-9.6]; p=0.001) and allele A (diabetic/pre-diabetic 31%, control 9%, (OR [95% CI]=4.67[2-10.7]) was significantly more in diabetic/pre-diabetic group compared to control group. The difference in the genotype frequency for rs266729 in diabetic group compared to that in control was not significant. The levels of adiponectin in diabetic cases had no difference compared to the control group in both polymorphisms. The rs266729 was not associated with any metabolic parameter except waist circumference (p=0.03), however, rs17300539 shows association only with fasting plasma glucose, triglyceride, and total cholesterol (p=0.007, 0.039, 0.0032, respectively). Conclusion. Our findings showed that there is an association between rs17300539 with the increase of T2DM but rs266729 showed no association with the risk of T2DM. Allele A of rs17300539 increased the risk of diabetes. There is no association between adiponectin level and both polymorphisms.
format	article
author	Karimi Helma Nezhadali Masoumeh Hedayati Mehdi
author_facet	Karimi Helma Nezhadali Masoumeh Hedayati Mehdi
author_sort	Karimi Helma
title	Association between adiponectin rs17300539 and rs266729 gene polymorphisms with serum adiponectin level in an Iranian diabetic/pre-diabetic population
title_short	Association between adiponectin rs17300539 and rs266729 gene polymorphisms with serum adiponectin level in an Iranian diabetic/pre-diabetic population
title_full	Association between adiponectin rs17300539 and rs266729 gene polymorphisms with serum adiponectin level in an Iranian diabetic/pre-diabetic population
title_fullStr	Association between adiponectin rs17300539 and rs266729 gene polymorphisms with serum adiponectin level in an Iranian diabetic/pre-diabetic population
title_full_unstemmed	Association between adiponectin rs17300539 and rs266729 gene polymorphisms with serum adiponectin level in an Iranian diabetic/pre-diabetic population
title_sort	association between adiponectin rs17300539 and rs266729 gene polymorphisms with serum adiponectin level in an iranian diabetic/pre-diabetic population
publisher	Sciendo
publishDate	2018
url	https://doaj.org/article/d09009732f424ee79fbad37e5b655126
work_keys_str_mv	AT karimihelma associationbetweenadiponectinrs17300539andrs266729genepolymorphismswithserumadiponectinlevelinaniraniandiabeticprediabeticpopulation AT nezhadalimasoumeh associationbetweenadiponectinrs17300539andrs266729genepolymorphismswithserumadiponectinlevelinaniraniandiabeticprediabeticpopulation AT hedayatimehdi associationbetweenadiponectinrs17300539andrs266729genepolymorphismswithserumadiponectinlevelinaniraniandiabeticprediabeticpopulation
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Association between adiponectin rs17300539 and rs266729 gene polymorphisms with serum adiponectin level in an Iranian diabetic/pre-diabetic population

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