Mouse lymphatic endothelial cell targeted probes: anti-LYVE-1 antibody-based magnetic nanoparticles

Qiu Guo,1,2,* Yi Liu,1,* Ke Xu,1 Ke Ren,1 WenGe Sun1 1Department of Radiology, The First Hospital of China Medical University, Shenyang, Liaoning, People's Republic of China; 2Key Laboratory of Imaging Diagnosis and Interventional Radiology of Liaoning Province, Shenyang, Liaoning, People&am...

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Autores principales:	Guo Q, Liu Y, Xu K, Ren K, Sun WG
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2013
Materias:	Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/d0909a2d358149889e7d5136a955db3a
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spelling	oai:doaj.org-article:d0909a2d358149889e7d5136a955db3a2021-12-02T02:30:50ZMouse lymphatic endothelial cell targeted probes: anti-LYVE-1 antibody-based magnetic nanoparticles1176-91141178-2013https://doaj.org/article/d0909a2d358149889e7d5136a955db3a2013-06-01T00:00:00Zhttp://www.dovepress.com/mouse-lymphatic-endothelial-cell-targeted-probes-anti-lyve-1-antibody--a13429https://doaj.org/toc/1176-9114https://doaj.org/toc/1178-2013Qiu Guo,1,2,* Yi Liu,1,* Ke Xu,1 Ke Ren,1 WenGe Sun1 1Department of Radiology, The First Hospital of China Medical University, Shenyang, Liaoning, People's Republic of China; 2Key Laboratory of Imaging Diagnosis and Interventional Radiology of Liaoning Province, Shenyang, Liaoning, People's Republic of China *These authors contributed equally to this work Purpose: To investigate the specific targeting property of lymphatic vessel endothelial hyaluronan receptor-1 binding polyethylene glycol-coated ultrasmall superparamagnetic iron oxide (LYVE-1-PEG-USPIO) nanoparticles to mouse lymphatic endothelial cells (MLECs). Methods: A ligand specific target to lymphatic vessels was selected by immunohistochemical staining on the sections of a Lewis subcutaneous transplanted tumor. The z-average hydrodynamic diameter (HD), zeta potential, and the relaxivity of PEG-USPIO and LYVE-1-PEG-USPIO nanoparticles were determined with a laser particle analyzer and magnetic resonance T2 spin echo sequence, respectively. Prussian blue staining and transmission electron microscopy (TEM) of nanoparticle labeled cells were performed to determine the nanoparticles' binding form. Magnetic resonance imaging (MRI) was performed in vitro to evaluate the signal enhancement on the T2 spin echo sequence of the nanoparticle labeled cells. The iron content of the labeled cells after the Prussian blue staining and MRI scanning was determined by atomic absorption spectroscopy (AAS). Results: The anti-LYVE-1 antibody was used as the specific ligand to synthesize the target probe to the MLECs. The mean z-average HDs of the LYVE-1-PEG-USPIO and PEG-USPIO nanoparticles were 57.42 ± 0.31 nm and 47.91 ± 0.73 nm, respectively, and the mean zeta potentials of the LYVE-1-PEG-USPIO and PEG-USPIO nanoparticles were 12.38 ± 4.87 mV and 2.57 ± 0.83 mV, respectively. The relaxivities of the LYVE-1-PEG-USPIO and PEG-USPIO nanoparticles were 185.48 mM-1s-1 and 608.32 mM-1s-1. Cells binding nanoparticles were visualized as blue granules in the Prussian blue staining. The TEM results of the labeled cells showed the specific localization of nanoparticles. The AAS results of labeled cells after the Prussian blue staining and MRI scanning showed that the LYVE-1-PEG-USPIO nanoparticles had good binding selectivity for MLECs. MRI results indicated that the PEG-USPIO and LYVE-1-PEG-USPIO nanoparticles could generate contrast on T2-weighted imaging, and the correlation between R2 and the iron content of the labeled cells was significantly positive. Conclusion: This study demonstrated that LYVE-1-PEG-USPIO nanoparticles might potentially be used as an MRI contrast agent for targeting MLECs, and the magnetic properties of LYVE-1-PEG-USPIO nanoparticles were suitable for MRI. Keywords: nanoparticles, lymphatic vessel endothelial hyaluronan receptor-1 (LYVE-1), ultrasmall superparamagnetic iron oxide (USPIO), mouse lymphatic endothelial cells (MLECs), magnetic resonance imaging (MRI)Guo QLiu YXu KRen KSun WGDove Medical PressarticleMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2013, Iss default, Pp 2273-2284 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine (General) R5-920
spellingShingle	Medicine (General) R5-920 Guo Q Liu Y Xu K Ren K Sun WG Mouse lymphatic endothelial cell targeted probes: anti-LYVE-1 antibody-based magnetic nanoparticles
description	Qiu Guo,1,2,* Yi Liu,1,* Ke Xu,1 Ke Ren,1 WenGe Sun1 1Department of Radiology, The First Hospital of China Medical University, Shenyang, Liaoning, People's Republic of China; 2Key Laboratory of Imaging Diagnosis and Interventional Radiology of Liaoning Province, Shenyang, Liaoning, People's Republic of China *These authors contributed equally to this work Purpose: To investigate the specific targeting property of lymphatic vessel endothelial hyaluronan receptor-1 binding polyethylene glycol-coated ultrasmall superparamagnetic iron oxide (LYVE-1-PEG-USPIO) nanoparticles to mouse lymphatic endothelial cells (MLECs). Methods: A ligand specific target to lymphatic vessels was selected by immunohistochemical staining on the sections of a Lewis subcutaneous transplanted tumor. The z-average hydrodynamic diameter (HD), zeta potential, and the relaxivity of PEG-USPIO and LYVE-1-PEG-USPIO nanoparticles were determined with a laser particle analyzer and magnetic resonance T2 spin echo sequence, respectively. Prussian blue staining and transmission electron microscopy (TEM) of nanoparticle labeled cells were performed to determine the nanoparticles' binding form. Magnetic resonance imaging (MRI) was performed in vitro to evaluate the signal enhancement on the T2 spin echo sequence of the nanoparticle labeled cells. The iron content of the labeled cells after the Prussian blue staining and MRI scanning was determined by atomic absorption spectroscopy (AAS). Results: The anti-LYVE-1 antibody was used as the specific ligand to synthesize the target probe to the MLECs. The mean z-average HDs of the LYVE-1-PEG-USPIO and PEG-USPIO nanoparticles were 57.42 ± 0.31 nm and 47.91 ± 0.73 nm, respectively, and the mean zeta potentials of the LYVE-1-PEG-USPIO and PEG-USPIO nanoparticles were 12.38 ± 4.87 mV and 2.57 ± 0.83 mV, respectively. The relaxivities of the LYVE-1-PEG-USPIO and PEG-USPIO nanoparticles were 185.48 mM-1s-1 and 608.32 mM-1s-1. Cells binding nanoparticles were visualized as blue granules in the Prussian blue staining. The TEM results of the labeled cells showed the specific localization of nanoparticles. The AAS results of labeled cells after the Prussian blue staining and MRI scanning showed that the LYVE-1-PEG-USPIO nanoparticles had good binding selectivity for MLECs. MRI results indicated that the PEG-USPIO and LYVE-1-PEG-USPIO nanoparticles could generate contrast on T2-weighted imaging, and the correlation between R2 and the iron content of the labeled cells was significantly positive. Conclusion: This study demonstrated that LYVE-1-PEG-USPIO nanoparticles might potentially be used as an MRI contrast agent for targeting MLECs, and the magnetic properties of LYVE-1-PEG-USPIO nanoparticles were suitable for MRI. Keywords: nanoparticles, lymphatic vessel endothelial hyaluronan receptor-1 (LYVE-1), ultrasmall superparamagnetic iron oxide (USPIO), mouse lymphatic endothelial cells (MLECs), magnetic resonance imaging (MRI)
format	article
author	Guo Q Liu Y Xu K Ren K Sun WG
author_facet	Guo Q Liu Y Xu K Ren K Sun WG
author_sort	Guo Q
title	Mouse lymphatic endothelial cell targeted probes: anti-LYVE-1 antibody-based magnetic nanoparticles
title_short	Mouse lymphatic endothelial cell targeted probes: anti-LYVE-1 antibody-based magnetic nanoparticles
title_full	Mouse lymphatic endothelial cell targeted probes: anti-LYVE-1 antibody-based magnetic nanoparticles
title_fullStr	Mouse lymphatic endothelial cell targeted probes: anti-LYVE-1 antibody-based magnetic nanoparticles
title_full_unstemmed	Mouse lymphatic endothelial cell targeted probes: anti-LYVE-1 antibody-based magnetic nanoparticles
title_sort	mouse lymphatic endothelial cell targeted probes: anti-lyve-1 antibody-based magnetic nanoparticles
publisher	Dove Medical Press
publishDate	2013
url	https://doaj.org/article/d0909a2d358149889e7d5136a955db3a
work_keys_str_mv	AT guoq mouselymphaticendothelialcelltargetedprobesantilyve1antibodybasedmagneticnanoparticles AT liuy mouselymphaticendothelialcelltargetedprobesantilyve1antibodybasedmagneticnanoparticles AT xuk mouselymphaticendothelialcelltargetedprobesantilyve1antibodybasedmagneticnanoparticles AT renk mouselymphaticendothelialcelltargetedprobesantilyve1antibodybasedmagneticnanoparticles AT sunwg mouselymphaticendothelialcelltargetedprobesantilyve1antibodybasedmagneticnanoparticles
_version_	1718402439042826240

Mouse lymphatic endothelial cell targeted probes: anti-LYVE-1 antibody-based magnetic nanoparticles

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