Modern Acinetobacter baumannii clinical isolates replicate inside spacious vacuoles and egress from macrophages.

Multidrug-resistant Acinetobacter baumannii infections are increasing at alarming rates. Therefore, novel antibiotic-sparing treatments to combat these A. baumannii infections are urgently needed. The development of these interventions would benefit from a better understanding of this bacterium'...

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Autores principales:	Gabriela Sycz, Gisela Di Venanzio, Jesus S Distel, Mariana G Sartorio, Nguyen-Hung Le, Nichollas E Scott, Wandy L Beatty, Mario F Feldman
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2021
Materias:	Immunologic diseases. Allergy RC581-607 Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/d09b4c9b97c44eaaab6d066fa2a98b86
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spelling	oai:doaj.org-article:d09b4c9b97c44eaaab6d066fa2a98b862021-12-02T20:00:24ZModern Acinetobacter baumannii clinical isolates replicate inside spacious vacuoles and egress from macrophages.1553-73661553-737410.1371/journal.ppat.1009802https://doaj.org/article/d09b4c9b97c44eaaab6d066fa2a98b862021-08-01T00:00:00Zhttps://doi.org/10.1371/journal.ppat.1009802https://doaj.org/toc/1553-7366https://doaj.org/toc/1553-7374Multidrug-resistant Acinetobacter baumannii infections are increasing at alarming rates. Therefore, novel antibiotic-sparing treatments to combat these A. baumannii infections are urgently needed. The development of these interventions would benefit from a better understanding of this bacterium's pathobiology, which remains poorly understood. A. baumannii is regarded as an extracellular opportunistic pathogen. However, research on Acinetobacter has largely focused on common lab strains, such as ATCC 19606, that have been isolated several decades ago. These strains exhibit reduced virulence when compared to recently isolated clinical strains. In this work, we demonstrate that, unlike ATCC 19606, several modern A. baumannii clinical isolates, including the recent clinical urinary isolate UPAB1, persist and replicate inside macrophages within spacious vacuoles. We show that intracellular replication of UPAB1 is dependent on a functional type I secretion system (T1SS) and pAB5, a large conjugative plasmid that controls the expression of several chromosomally-encoded genes. Finally, we show that UPAB1 escapes from the infected macrophages by a lytic process. To our knowledge, this is the first report of intracellular growth and replication of A. baumannii. We suggest that intracellular replication within macrophages may contribute to evasion of the immune response, dissemination, and antibiotic tolerance of A. baumannii.Gabriela SyczGisela Di VenanzioJesus S DistelMariana G SartorioNguyen-Hung LeNichollas E ScottWandy L BeattyMario F FeldmanPublic Library of Science (PLoS)articleImmunologic diseases. AllergyRC581-607Biology (General)QH301-705.5ENPLoS Pathogens, Vol 17, Iss 8, p e1009802 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Immunologic diseases. Allergy RC581-607 Biology (General) QH301-705.5
spellingShingle	Immunologic diseases. Allergy RC581-607 Biology (General) QH301-705.5 Gabriela Sycz Gisela Di Venanzio Jesus S Distel Mariana G Sartorio Nguyen-Hung Le Nichollas E Scott Wandy L Beatty Mario F Feldman Modern Acinetobacter baumannii clinical isolates replicate inside spacious vacuoles and egress from macrophages.
description	Multidrug-resistant Acinetobacter baumannii infections are increasing at alarming rates. Therefore, novel antibiotic-sparing treatments to combat these A. baumannii infections are urgently needed. The development of these interventions would benefit from a better understanding of this bacterium's pathobiology, which remains poorly understood. A. baumannii is regarded as an extracellular opportunistic pathogen. However, research on Acinetobacter has largely focused on common lab strains, such as ATCC 19606, that have been isolated several decades ago. These strains exhibit reduced virulence when compared to recently isolated clinical strains. In this work, we demonstrate that, unlike ATCC 19606, several modern A. baumannii clinical isolates, including the recent clinical urinary isolate UPAB1, persist and replicate inside macrophages within spacious vacuoles. We show that intracellular replication of UPAB1 is dependent on a functional type I secretion system (T1SS) and pAB5, a large conjugative plasmid that controls the expression of several chromosomally-encoded genes. Finally, we show that UPAB1 escapes from the infected macrophages by a lytic process. To our knowledge, this is the first report of intracellular growth and replication of A. baumannii. We suggest that intracellular replication within macrophages may contribute to evasion of the immune response, dissemination, and antibiotic tolerance of A. baumannii.
format	article
author	Gabriela Sycz Gisela Di Venanzio Jesus S Distel Mariana G Sartorio Nguyen-Hung Le Nichollas E Scott Wandy L Beatty Mario F Feldman
author_facet	Gabriela Sycz Gisela Di Venanzio Jesus S Distel Mariana G Sartorio Nguyen-Hung Le Nichollas E Scott Wandy L Beatty Mario F Feldman
author_sort	Gabriela Sycz
title	Modern Acinetobacter baumannii clinical isolates replicate inside spacious vacuoles and egress from macrophages.
title_short	Modern Acinetobacter baumannii clinical isolates replicate inside spacious vacuoles and egress from macrophages.
title_full	Modern Acinetobacter baumannii clinical isolates replicate inside spacious vacuoles and egress from macrophages.
title_fullStr	Modern Acinetobacter baumannii clinical isolates replicate inside spacious vacuoles and egress from macrophages.
title_full_unstemmed	Modern Acinetobacter baumannii clinical isolates replicate inside spacious vacuoles and egress from macrophages.
title_sort	modern acinetobacter baumannii clinical isolates replicate inside spacious vacuoles and egress from macrophages.
publisher	Public Library of Science (PLoS)
publishDate	2021
url	https://doaj.org/article/d09b4c9b97c44eaaab6d066fa2a98b86
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Modern Acinetobacter baumannii clinical isolates replicate inside spacious vacuoles and egress from macrophages.

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