Vertical sleeve gastrectomy reverses diet-induced gene-regulatory changes impacting lipid metabolism

Abstract Vertical sleeve gastrectomy (VSG) produces sustainable weight loss, remission of type 2 diabetes (T2D), and improvement of nonalcoholic fatty liver disease (NAFLD). However, the molecular mechanisms underlying the metabolic benefits of VSG have remained elusive. According to our previous re...

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Autores principales:	Juan Du, Jingyan Tian, Lili Ding, Candi Trac, Brian Xia, Siming Sun, Dustin E. Schones, Wendong Huang
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/d0a659529ecd44d188fc16d8b8d385fd
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spelling	oai:doaj.org-article:d0a659529ecd44d188fc16d8b8d385fd2021-12-02T16:06:07ZVertical sleeve gastrectomy reverses diet-induced gene-regulatory changes impacting lipid metabolism10.1038/s41598-017-05349-22045-2322https://doaj.org/article/d0a659529ecd44d188fc16d8b8d385fd2017-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-05349-2https://doaj.org/toc/2045-2322Abstract Vertical sleeve gastrectomy (VSG) produces sustainable weight loss, remission of type 2 diabetes (T2D), and improvement of nonalcoholic fatty liver disease (NAFLD). However, the molecular mechanisms underlying the metabolic benefits of VSG have remained elusive. According to our previous results, diet-induced obesity induces epigenetic modifications to chromatin in mouse liver. We demonstrate here that VSG in C57BL/6J wild-type male mice can reverse these chromatin modifications and thereby impact the expression of key metabolic genes. Genes involved in lipid metabolism, especially omega-6 fatty acid metabolism, are up-regulated in livers of mice after VSG while genes in inflammatory pathways are down-regulated after VSG. Consistent with gene expression changes, regulatory regions near genes involved in inflammatory response displayed decreased chromatin accessibility after VSG. Our results indicate that VSG induces global regulatory changes that impact hepatic inflammatory and lipid metabolic pathways, providing new insight into the mechanisms underlying the beneficial metabolic effects induced by VSG.Juan DuJingyan TianLili DingCandi TracBrian XiaSiming SunDustin E. SchonesWendong HuangNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-10 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Juan Du Jingyan Tian Lili Ding Candi Trac Brian Xia Siming Sun Dustin E. Schones Wendong Huang Vertical sleeve gastrectomy reverses diet-induced gene-regulatory changes impacting lipid metabolism
description	Abstract Vertical sleeve gastrectomy (VSG) produces sustainable weight loss, remission of type 2 diabetes (T2D), and improvement of nonalcoholic fatty liver disease (NAFLD). However, the molecular mechanisms underlying the metabolic benefits of VSG have remained elusive. According to our previous results, diet-induced obesity induces epigenetic modifications to chromatin in mouse liver. We demonstrate here that VSG in C57BL/6J wild-type male mice can reverse these chromatin modifications and thereby impact the expression of key metabolic genes. Genes involved in lipid metabolism, especially omega-6 fatty acid metabolism, are up-regulated in livers of mice after VSG while genes in inflammatory pathways are down-regulated after VSG. Consistent with gene expression changes, regulatory regions near genes involved in inflammatory response displayed decreased chromatin accessibility after VSG. Our results indicate that VSG induces global regulatory changes that impact hepatic inflammatory and lipid metabolic pathways, providing new insight into the mechanisms underlying the beneficial metabolic effects induced by VSG.
format	article
author	Juan Du Jingyan Tian Lili Ding Candi Trac Brian Xia Siming Sun Dustin E. Schones Wendong Huang
author_facet	Juan Du Jingyan Tian Lili Ding Candi Trac Brian Xia Siming Sun Dustin E. Schones Wendong Huang
author_sort	Juan Du
title	Vertical sleeve gastrectomy reverses diet-induced gene-regulatory changes impacting lipid metabolism
title_short	Vertical sleeve gastrectomy reverses diet-induced gene-regulatory changes impacting lipid metabolism
title_full	Vertical sleeve gastrectomy reverses diet-induced gene-regulatory changes impacting lipid metabolism
title_fullStr	Vertical sleeve gastrectomy reverses diet-induced gene-regulatory changes impacting lipid metabolism
title_full_unstemmed	Vertical sleeve gastrectomy reverses diet-induced gene-regulatory changes impacting lipid metabolism
title_sort	vertical sleeve gastrectomy reverses diet-induced gene-regulatory changes impacting lipid metabolism
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/d0a659529ecd44d188fc16d8b8d385fd
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Vertical sleeve gastrectomy reverses diet-induced gene-regulatory changes impacting lipid metabolism

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