High Prevalence of Colistin-Resistant <named-content content-type="genus-species">Escherichia coli</named-content> with Chromosomally Carried <italic toggle="yes">mcr-1</italic> in Healthy Residents in Vietnam

ABSTRACT The wide distribution of colistin-resistant bacteria in developing countries has become a common phenomenon. To understand the mechanisms underlying their distribution, we studied the mcr genetic background of colistin-resistant Escherichia coli isolates from the fecal microbiota of healthy...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Takahiro Yamaguchi, Ryuji Kawahara, Kouta Hamamoto, Itaru Hirai, Diep Thi Khong, Thang Nam Nguyen, Hoa Thi Tran, Daisuke Motooka, Shota Nakamura, Yoshimasa Yamamoto
Formato:	article
Lenguaje:	EN
Publicado:	American Society for Microbiology 2020
Materias:	Escherichia coli Vietnam chromosomal mcr colistin resistance residents Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/d0b6a57111b64bc88d6497c6ec8a31ae
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:d0b6a57111b64bc88d6497c6ec8a31ae
record_format	dspace
spelling	oai:doaj.org-article:d0b6a57111b64bc88d6497c6ec8a31ae2021-11-15T15:29:16ZHigh Prevalence of Colistin-Resistant <named-content content-type="genus-species">Escherichia coli</named-content> with Chromosomally Carried <italic toggle="yes">mcr-1</italic> in Healthy Residents in Vietnam10.1128/mSphere.00117-202379-5042https://doaj.org/article/d0b6a57111b64bc88d6497c6ec8a31ae2020-04-01T00:00:00Zhttps://journals.asm.org/doi/10.1128/mSphere.00117-20https://doaj.org/toc/2379-5042ABSTRACT The wide distribution of colistin-resistant bacteria in developing countries has become a common phenomenon. To understand the mechanisms underlying their distribution, we studied the mcr genetic background of colistin-resistant Escherichia coli isolates from the fecal microbiota of healthy human residents from a community in Vietnam with a high prevalence of colistin-resistant E. coli with mcr. Fifty-seven colistin-resistant isolates were obtained from 98 residents; one isolate was collected from each individual and analyzed for mcr. We found that 36.8% of the isolates carried chromosomal mcr-1. Further, 63.2% and 1.8% of the isolates carried mcr-1 on the plasmid and the plasmid/chromosome, respectively. Whole-genome sequencing of genetically unrelated isolates showed that the majority (6 of 7) of the isolates had the chromosomal mcr-1 in a complete ancestral mcr-1 transposon Tn6330, ISApl1-mcr-1-PAP2-ISApl1, which was inserted at various positions on the chromosomes. In addition, the majority (87.5%) of Tn6330 of mcr-1-carrying plasmids (n = 8) lacked both upstream and downstream ISApl1 transposons. The results obtained in this study indicate that plasmid-to-chromosomal transfer of mcr-1 may have occurred recently in the fecal microbiota of the residents. Additionally, Tn6330 on the chromosome may lose ISApl1 from the transposon during multiplication to gain a more stable mcr-1 state on the chromosome. Stabilization of resistance by the chromosomal incorporation of mcr-1 would be an additional challenge in combating the dissemination of resistant bacteria. IMPORTANCE Elucidation of the mechanism of the wide dissemination of colistin-resistant bacteria in communities of developing countries is an urgent public health issue. In this study, we investigated the genetic background of the colistin resistance gene mcr in E. coli isolates from the fecal microbiota of healthy human residents living in a community in Vietnam with a high prevalence of colistin-resistant E. coli. Our study revealed for the first time, a surprisingly high percentage (36.8%) of colistin-resistant E. coli carrying chromosomal mcr-1, the emergence of which may have occurred recently, in the fecal microbiota of the community residents. The mcr-1 transposon on the chromosome may develop into a more stable genotype by the loss of insertion sequences (ISs). Our results are valuable in understanding the mechanism underlying the increasing prevalence of colistin-resistant bacteria within a community.Takahiro YamaguchiRyuji KawaharaKouta HamamotoItaru HiraiDiep Thi KhongThang Nam NguyenHoa Thi TranDaisuke MotookaShota NakamuraYoshimasa YamamotoAmerican Society for MicrobiologyarticleEscherichia coliVietnamchromosomal mcrcolistin resistanceresidentsMicrobiologyQR1-502ENmSphere, Vol 5, Iss 2 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	Escherichia coli Vietnam chromosomal mcr colistin resistance residents Microbiology QR1-502
spellingShingle	Escherichia coli Vietnam chromosomal mcr colistin resistance residents Microbiology QR1-502 Takahiro Yamaguchi Ryuji Kawahara Kouta Hamamoto Itaru Hirai Diep Thi Khong Thang Nam Nguyen Hoa Thi Tran Daisuke Motooka Shota Nakamura Yoshimasa Yamamoto High Prevalence of Colistin-Resistant <named-content content-type="genus-species">Escherichia coli</named-content> with Chromosomally Carried <italic toggle="yes">mcr-1</italic> in Healthy Residents in Vietnam
description	ABSTRACT The wide distribution of colistin-resistant bacteria in developing countries has become a common phenomenon. To understand the mechanisms underlying their distribution, we studied the mcr genetic background of colistin-resistant Escherichia coli isolates from the fecal microbiota of healthy human residents from a community in Vietnam with a high prevalence of colistin-resistant E. coli with mcr. Fifty-seven colistin-resistant isolates were obtained from 98 residents; one isolate was collected from each individual and analyzed for mcr. We found that 36.8% of the isolates carried chromosomal mcr-1. Further, 63.2% and 1.8% of the isolates carried mcr-1 on the plasmid and the plasmid/chromosome, respectively. Whole-genome sequencing of genetically unrelated isolates showed that the majority (6 of 7) of the isolates had the chromosomal mcr-1 in a complete ancestral mcr-1 transposon Tn6330, ISApl1-mcr-1-PAP2-ISApl1, which was inserted at various positions on the chromosomes. In addition, the majority (87.5%) of Tn6330 of mcr-1-carrying plasmids (n = 8) lacked both upstream and downstream ISApl1 transposons. The results obtained in this study indicate that plasmid-to-chromosomal transfer of mcr-1 may have occurred recently in the fecal microbiota of the residents. Additionally, Tn6330 on the chromosome may lose ISApl1 from the transposon during multiplication to gain a more stable mcr-1 state on the chromosome. Stabilization of resistance by the chromosomal incorporation of mcr-1 would be an additional challenge in combating the dissemination of resistant bacteria. IMPORTANCE Elucidation of the mechanism of the wide dissemination of colistin-resistant bacteria in communities of developing countries is an urgent public health issue. In this study, we investigated the genetic background of the colistin resistance gene mcr in E. coli isolates from the fecal microbiota of healthy human residents living in a community in Vietnam with a high prevalence of colistin-resistant E. coli. Our study revealed for the first time, a surprisingly high percentage (36.8%) of colistin-resistant E. coli carrying chromosomal mcr-1, the emergence of which may have occurred recently, in the fecal microbiota of the community residents. The mcr-1 transposon on the chromosome may develop into a more stable genotype by the loss of insertion sequences (ISs). Our results are valuable in understanding the mechanism underlying the increasing prevalence of colistin-resistant bacteria within a community.
format	article
author	Takahiro Yamaguchi Ryuji Kawahara Kouta Hamamoto Itaru Hirai Diep Thi Khong Thang Nam Nguyen Hoa Thi Tran Daisuke Motooka Shota Nakamura Yoshimasa Yamamoto
author_facet	Takahiro Yamaguchi Ryuji Kawahara Kouta Hamamoto Itaru Hirai Diep Thi Khong Thang Nam Nguyen Hoa Thi Tran Daisuke Motooka Shota Nakamura Yoshimasa Yamamoto
author_sort	Takahiro Yamaguchi
title	High Prevalence of Colistin-Resistant <named-content content-type="genus-species">Escherichia coli</named-content> with Chromosomally Carried <italic toggle="yes">mcr-1</italic> in Healthy Residents in Vietnam
title_short	High Prevalence of Colistin-Resistant <named-content content-type="genus-species">Escherichia coli</named-content> with Chromosomally Carried <italic toggle="yes">mcr-1</italic> in Healthy Residents in Vietnam
title_full	High Prevalence of Colistin-Resistant <named-content content-type="genus-species">Escherichia coli</named-content> with Chromosomally Carried <italic toggle="yes">mcr-1</italic> in Healthy Residents in Vietnam
title_fullStr	High Prevalence of Colistin-Resistant <named-content content-type="genus-species">Escherichia coli</named-content> with Chromosomally Carried <italic toggle="yes">mcr-1</italic> in Healthy Residents in Vietnam
title_full_unstemmed	High Prevalence of Colistin-Resistant <named-content content-type="genus-species">Escherichia coli</named-content> with Chromosomally Carried <italic toggle="yes">mcr-1</italic> in Healthy Residents in Vietnam
title_sort	high prevalence of colistin-resistant <named-content content-type="genus-species">escherichia coli</named-content> with chromosomally carried <italic toggle="yes">mcr-1</italic> in healthy residents in vietnam
publisher	American Society for Microbiology
publishDate	2020
url	https://doaj.org/article/d0b6a57111b64bc88d6497c6ec8a31ae
work_keys_str_mv	AT takahiroyamaguchi highprevalenceofcolistinresistantnamedcontentcontenttypegenusspeciesescherichiacolinamedcontentwithchromosomallycarrieditalictoggleyesmcr1italicinhealthyresidentsinvietnam AT ryujikawahara highprevalenceofcolistinresistantnamedcontentcontenttypegenusspeciesescherichiacolinamedcontentwithchromosomallycarrieditalictoggleyesmcr1italicinhealthyresidentsinvietnam AT koutahamamoto highprevalenceofcolistinresistantnamedcontentcontenttypegenusspeciesescherichiacolinamedcontentwithchromosomallycarrieditalictoggleyesmcr1italicinhealthyresidentsinvietnam AT itaruhirai highprevalenceofcolistinresistantnamedcontentcontenttypegenusspeciesescherichiacolinamedcontentwithchromosomallycarrieditalictoggleyesmcr1italicinhealthyresidentsinvietnam AT diepthikhong highprevalenceofcolistinresistantnamedcontentcontenttypegenusspeciesescherichiacolinamedcontentwithchromosomallycarrieditalictoggleyesmcr1italicinhealthyresidentsinvietnam AT thangnamnguyen highprevalenceofcolistinresistantnamedcontentcontenttypegenusspeciesescherichiacolinamedcontentwithchromosomallycarrieditalictoggleyesmcr1italicinhealthyresidentsinvietnam AT hoathitran highprevalenceofcolistinresistantnamedcontentcontenttypegenusspeciesescherichiacolinamedcontentwithchromosomallycarrieditalictoggleyesmcr1italicinhealthyresidentsinvietnam AT daisukemotooka highprevalenceofcolistinresistantnamedcontentcontenttypegenusspeciesescherichiacolinamedcontentwithchromosomallycarrieditalictoggleyesmcr1italicinhealthyresidentsinvietnam AT shotanakamura highprevalenceofcolistinresistantnamedcontentcontenttypegenusspeciesescherichiacolinamedcontentwithchromosomallycarrieditalictoggleyesmcr1italicinhealthyresidentsinvietnam AT yoshimasayamamoto highprevalenceofcolistinresistantnamedcontentcontenttypegenusspeciesescherichiacolinamedcontentwithchromosomallycarrieditalictoggleyesmcr1italicinhealthyresidentsinvietnam
_version_	1718427914702159872

High Prevalence of Colistin-Resistant <named-content content-type="genus-species">Escherichia coli</named-content> with Chromosomally Carried <italic toggle="yes">mcr-1</italic> in Healthy Residents in Vietnam

Ejemplares similares