Role of the Discriminator Sequence in the Supercoiling Sensitivity of Bacterial Promoters

In this study, we highlight the role of the discriminator as a global sensor of supercoiling variations and propose the first quantitative regulatory model of this principle, based on the specific step of promoter opening during transcription initiation. It defines the predictive rule by which SC q...

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Autores principales:	Raphaël Forquet, Maïwenn Pineau, William Nasser, Sylvie Reverchon, Sam Meyer
Formato:	article
Lenguaje:	EN
Publicado:	American Society for Microbiology 2021
Materias:	Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/d0bf0838a738433d8d5e1ede648a5d8f
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spelling	oai:doaj.org-article:d0bf0838a738433d8d5e1ede648a5d8f2021-12-02T19:35:56ZRole of the Discriminator Sequence in the Supercoiling Sensitivity of Bacterial Promoters2379-507710.1128/mSystems.00978-21https://doaj.org/article/d0bf0838a738433d8d5e1ede648a5d8f2021-08-01T00:00:00Zhttps://journals.asm.org/doi/10.1128/mSystems.00978-21https://doaj.org/toc/2379-5077 In this study, we highlight the role of the discriminator as a global sensor of supercoiling variations and propose the first quantitative regulatory model of this principle, based on the specific step of promoter opening during transcription initiation. It defines the predictive rule by which SC quantitatively modulates the expression rate of bacterial promoters, depending on the G/C content of their discriminator and independently from promoter-specific regulatory proteins.Raphaël ForquetMaïwenn PineauWilliam NasserSylvie ReverchonSam MeyerAmerican Society for MicrobiologyarticleMicrobiologyQR1-502ENmSystems, Vol 6, Iss 4 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Microbiology QR1-502
spellingShingle	Microbiology QR1-502 Raphaël Forquet Maïwenn Pineau William Nasser Sylvie Reverchon Sam Meyer Role of the Discriminator Sequence in the Supercoiling Sensitivity of Bacterial Promoters
description	In this study, we highlight the role of the discriminator as a global sensor of supercoiling variations and propose the first quantitative regulatory model of this principle, based on the specific step of promoter opening during transcription initiation. It defines the predictive rule by which SC quantitatively modulates the expression rate of bacterial promoters, depending on the G/C content of their discriminator and independently from promoter-specific regulatory proteins.
format	article
author	Raphaël Forquet Maïwenn Pineau William Nasser Sylvie Reverchon Sam Meyer
author_facet	Raphaël Forquet Maïwenn Pineau William Nasser Sylvie Reverchon Sam Meyer
author_sort	Raphaël Forquet
title	Role of the Discriminator Sequence in the Supercoiling Sensitivity of Bacterial Promoters
title_short	Role of the Discriminator Sequence in the Supercoiling Sensitivity of Bacterial Promoters
title_full	Role of the Discriminator Sequence in the Supercoiling Sensitivity of Bacterial Promoters
title_fullStr	Role of the Discriminator Sequence in the Supercoiling Sensitivity of Bacterial Promoters
title_full_unstemmed	Role of the Discriminator Sequence in the Supercoiling Sensitivity of Bacterial Promoters
title_sort	role of the discriminator sequence in the supercoiling sensitivity of bacterial promoters
publisher	American Society for Microbiology
publishDate	2021
url	https://doaj.org/article/d0bf0838a738433d8d5e1ede648a5d8f
work_keys_str_mv	AT raphaelforquet roleofthediscriminatorsequenceinthesupercoilingsensitivityofbacterialpromoters AT maiwennpineau roleofthediscriminatorsequenceinthesupercoilingsensitivityofbacterialpromoters AT williamnasser roleofthediscriminatorsequenceinthesupercoilingsensitivityofbacterialpromoters AT sylviereverchon roleofthediscriminatorsequenceinthesupercoilingsensitivityofbacterialpromoters AT sammeyer roleofthediscriminatorsequenceinthesupercoilingsensitivityofbacterialpromoters
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Role of the Discriminator Sequence in the Supercoiling Sensitivity of Bacterial Promoters

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