Phenotypic expression of oral fibroblasts derived from oral submucous fibrosis: An assay through cell culture

Objective: The present study aimed to establish cell lines of fibroblast from human OSF tissues and their response to varying concentrations of arecoline. The various morphological forms of fibroblasts were identified to establish phenotypic change. Materials and Method: Fibroblast cell lines were o...

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Autores principales:	Abhishek Banerjee, Mathew O Mampilly, V V Kamath, Vijayaraghavan Athreya, Vijayalakshmi Kotrashetti, Kumar Chandan Srivastava, Deepti Shrivastava
Formato:	article
Lenguaje:	EN
Publicado:	Wolters Kluwer Medknow Publications 2021
Materias:	arecoline cell culture fibroblast phenotype oral submucous fibrosis Pharmacy and materia medica RS1-441 Analytical chemistry QD71-142
Acceso en línea:	https://doaj.org/article/d0c3667c553b4ceda39611deb26e9109
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spelling	oai:doaj.org-article:d0c3667c553b4ceda39611deb26e91092021-11-19T12:15:52ZPhenotypic expression of oral fibroblasts derived from oral submucous fibrosis: An assay through cell culture0975-740610.4103/jpbs.jpbs_408_21https://doaj.org/article/d0c3667c553b4ceda39611deb26e91092021-01-01T00:00:00Zhttp://www.jpbsonline.org/article.asp?issn=0975-7406;year=2021;volume=13;issue=6;spage=1234;epage=1239;aulast=Banerjeehttps://doaj.org/toc/0975-7406Objective: The present study aimed to establish cell lines of fibroblast from human OSF tissues and their response to varying concentrations of arecoline. The various morphological forms of fibroblasts were identified to establish phenotypic change. Materials and Method: Fibroblast cell lines were obtained from control samples as well as from OSF cases. The cell lines were treated with 50/100/150/300/500 ug/ml of arecoline and morphology were determined. Results: Three morphological forms were detected; F1 spindle, F2 epitheloid and the F3 stellate. The F3 to F1 ratio was higher in OSF. Arecoline at 50ug/ml was stimulatory and at 150ug/ml cytotoxic to the cell lines. Conclusion: Arecoline seems to enhance proliferation of the fibroblast at lower concentrations but cytotoxic at higher levels. This is probably due to the generation of new cell lines and response of the arecoline receptors indicating phenotypic change.Abhishek BanerjeeMathew O MampillyV V KamathVijayaraghavan AthreyaVijayalakshmi KotrashettiKumar Chandan SrivastavaDeepti ShrivastavaWolters Kluwer Medknow Publicationsarticlearecolinecell culturefibroblast phenotypeoral submucous fibrosisPharmacy and materia medicaRS1-441Analytical chemistryQD71-142ENJournal of Pharmacy and Bioallied Sciences, Vol 13, Iss 6, Pp 1234-1239 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	arecoline cell culture fibroblast phenotype oral submucous fibrosis Pharmacy and materia medica RS1-441 Analytical chemistry QD71-142
spellingShingle	arecoline cell culture fibroblast phenotype oral submucous fibrosis Pharmacy and materia medica RS1-441 Analytical chemistry QD71-142 Abhishek Banerjee Mathew O Mampilly V V Kamath Vijayaraghavan Athreya Vijayalakshmi Kotrashetti Kumar Chandan Srivastava Deepti Shrivastava Phenotypic expression of oral fibroblasts derived from oral submucous fibrosis: An assay through cell culture
description	Objective: The present study aimed to establish cell lines of fibroblast from human OSF tissues and their response to varying concentrations of arecoline. The various morphological forms of fibroblasts were identified to establish phenotypic change. Materials and Method: Fibroblast cell lines were obtained from control samples as well as from OSF cases. The cell lines were treated with 50/100/150/300/500 ug/ml of arecoline and morphology were determined. Results: Three morphological forms were detected; F1 spindle, F2 epitheloid and the F3 stellate. The F3 to F1 ratio was higher in OSF. Arecoline at 50ug/ml was stimulatory and at 150ug/ml cytotoxic to the cell lines. Conclusion: Arecoline seems to enhance proliferation of the fibroblast at lower concentrations but cytotoxic at higher levels. This is probably due to the generation of new cell lines and response of the arecoline receptors indicating phenotypic change.
format	article
author	Abhishek Banerjee Mathew O Mampilly V V Kamath Vijayaraghavan Athreya Vijayalakshmi Kotrashetti Kumar Chandan Srivastava Deepti Shrivastava
author_facet	Abhishek Banerjee Mathew O Mampilly V V Kamath Vijayaraghavan Athreya Vijayalakshmi Kotrashetti Kumar Chandan Srivastava Deepti Shrivastava
author_sort	Abhishek Banerjee
title	Phenotypic expression of oral fibroblasts derived from oral submucous fibrosis: An assay through cell culture
title_short	Phenotypic expression of oral fibroblasts derived from oral submucous fibrosis: An assay through cell culture
title_full	Phenotypic expression of oral fibroblasts derived from oral submucous fibrosis: An assay through cell culture
title_fullStr	Phenotypic expression of oral fibroblasts derived from oral submucous fibrosis: An assay through cell culture
title_full_unstemmed	Phenotypic expression of oral fibroblasts derived from oral submucous fibrosis: An assay through cell culture
title_sort	phenotypic expression of oral fibroblasts derived from oral submucous fibrosis: an assay through cell culture
publisher	Wolters Kluwer Medknow Publications
publishDate	2021
url	https://doaj.org/article/d0c3667c553b4ceda39611deb26e9109
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Phenotypic expression of oral fibroblasts derived from oral submucous fibrosis: An assay through cell culture

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