Immunoinformatics and Immunogenetics-Based Design of Immunogenic Peptides Vaccine against the Emerging Tick-Borne Encephalitis Virus (TBEV) and Its Validation through In Silico Cloning and Immune Simulation

Pegivirus, HPgV, earlier known as Gb virus and hepatitis G virus, is an enveloped, positive-stranded RNA and lymphotropic virus classified into the Flaviviridae family. The transmission routes primarily involve blood products, with infections worldwide, leading up to 25% of persistent infections. To...

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Autores principales:	Muhammad Suleman, Muhammad Tahir ul Qamar, Kiran, Samreen Rasool, Aneela Rasool, Aqel Albutti, Noorah Alsowayeh, Ameen S. S. Alwashmi, Mohammad Abdullah Aljasir, Sajjad Ahmad, Zahid Hussain, Muhammad Rizwan, Syed Shujait Ali, Abbas Khan, Dong-Qing Wei
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	Tick-borne encephalitis (TBE) proteome mining vaccine construct in silico cloning immune simulation Medicine R
Acceso en línea:	https://doaj.org/article/d0c671d475e144ab999527a760bdbdef
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spelling	oai:doaj.org-article:d0c671d475e144ab999527a760bdbdef2021-11-25T19:10:03ZImmunoinformatics and Immunogenetics-Based Design of Immunogenic Peptides Vaccine against the Emerging Tick-Borne Encephalitis Virus (TBEV) and Its Validation through In Silico Cloning and Immune Simulation10.3390/vaccines91112102076-393Xhttps://doaj.org/article/d0c671d475e144ab999527a760bdbdef2021-10-01T00:00:00Zhttps://www.mdpi.com/2076-393X/9/11/1210https://doaj.org/toc/2076-393XPegivirus, HPgV, earlier known as Gb virus and hepatitis G virus, is an enveloped, positive-stranded RNA and lymphotropic virus classified into the Flaviviridae family. The transmission routes primarily involve blood products, with infections worldwide, leading up to 25% of persistent infections. To date, no effective therapeutic means are available to resolve Pegivirus infections. Effective vaccine therapeutics are the best alternative to manage this disease and any associated potential pandemic. Thus, whole proteome-based mining of immunogenic peptides, i.e., CTL (cytotoxic T lymphocytes), HTL (helper T lymphocytes) and B cell epitopes were mapped to design a vaccine ensemble. Our investigation revealed that 29 different epitopes impart a critical role in immune response induction, which was also validated by exploring its physiochemical properties and experimental feasibility. In silico expression and host immune simulation using an agent-based modeling approach confirmed the induction of both primary and secondary immune factors such as IL, cytokines and antibodies. The current study warrants further lab experiments to demonstrate its efficacy and safety.Muhammad SulemanMuhammad Tahir ul QamarKiranSamreen RasoolAneela RasoolAqel AlbuttiNoorah AlsowayehAmeen S. S. AlwashmiMohammad Abdullah AljasirSajjad AhmadZahid HussainMuhammad RizwanSyed Shujait AliAbbas KhanDong-Qing WeiMDPI AGarticleTick-borne encephalitis (TBE)proteome miningvaccine constructin silico cloningimmune simulationMedicineRENVaccines, Vol 9, Iss 1210, p 1210 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Tick-borne encephalitis (TBE) proteome mining vaccine construct in silico cloning immune simulation Medicine R
spellingShingle	Tick-borne encephalitis (TBE) proteome mining vaccine construct in silico cloning immune simulation Medicine R Muhammad Suleman Muhammad Tahir ul Qamar Kiran Samreen Rasool Aneela Rasool Aqel Albutti Noorah Alsowayeh Ameen S. S. Alwashmi Mohammad Abdullah Aljasir Sajjad Ahmad Zahid Hussain Muhammad Rizwan Syed Shujait Ali Abbas Khan Dong-Qing Wei Immunoinformatics and Immunogenetics-Based Design of Immunogenic Peptides Vaccine against the Emerging Tick-Borne Encephalitis Virus (TBEV) and Its Validation through In Silico Cloning and Immune Simulation
description	Pegivirus, HPgV, earlier known as Gb virus and hepatitis G virus, is an enveloped, positive-stranded RNA and lymphotropic virus classified into the Flaviviridae family. The transmission routes primarily involve blood products, with infections worldwide, leading up to 25% of persistent infections. To date, no effective therapeutic means are available to resolve Pegivirus infections. Effective vaccine therapeutics are the best alternative to manage this disease and any associated potential pandemic. Thus, whole proteome-based mining of immunogenic peptides, i.e., CTL (cytotoxic T lymphocytes), HTL (helper T lymphocytes) and B cell epitopes were mapped to design a vaccine ensemble. Our investigation revealed that 29 different epitopes impart a critical role in immune response induction, which was also validated by exploring its physiochemical properties and experimental feasibility. In silico expression and host immune simulation using an agent-based modeling approach confirmed the induction of both primary and secondary immune factors such as IL, cytokines and antibodies. The current study warrants further lab experiments to demonstrate its efficacy and safety.
format	article
author	Muhammad Suleman Muhammad Tahir ul Qamar Kiran Samreen Rasool Aneela Rasool Aqel Albutti Noorah Alsowayeh Ameen S. S. Alwashmi Mohammad Abdullah Aljasir Sajjad Ahmad Zahid Hussain Muhammad Rizwan Syed Shujait Ali Abbas Khan Dong-Qing Wei
author_facet	Muhammad Suleman Muhammad Tahir ul Qamar Kiran Samreen Rasool Aneela Rasool Aqel Albutti Noorah Alsowayeh Ameen S. S. Alwashmi Mohammad Abdullah Aljasir Sajjad Ahmad Zahid Hussain Muhammad Rizwan Syed Shujait Ali Abbas Khan Dong-Qing Wei
author_sort	Muhammad Suleman
title	Immunoinformatics and Immunogenetics-Based Design of Immunogenic Peptides Vaccine against the Emerging Tick-Borne Encephalitis Virus (TBEV) and Its Validation through In Silico Cloning and Immune Simulation
title_short	Immunoinformatics and Immunogenetics-Based Design of Immunogenic Peptides Vaccine against the Emerging Tick-Borne Encephalitis Virus (TBEV) and Its Validation through In Silico Cloning and Immune Simulation
title_full	Immunoinformatics and Immunogenetics-Based Design of Immunogenic Peptides Vaccine against the Emerging Tick-Borne Encephalitis Virus (TBEV) and Its Validation through In Silico Cloning and Immune Simulation
title_fullStr	Immunoinformatics and Immunogenetics-Based Design of Immunogenic Peptides Vaccine against the Emerging Tick-Borne Encephalitis Virus (TBEV) and Its Validation through In Silico Cloning and Immune Simulation
title_full_unstemmed	Immunoinformatics and Immunogenetics-Based Design of Immunogenic Peptides Vaccine against the Emerging Tick-Borne Encephalitis Virus (TBEV) and Its Validation through In Silico Cloning and Immune Simulation
title_sort	immunoinformatics and immunogenetics-based design of immunogenic peptides vaccine against the emerging tick-borne encephalitis virus (tbev) and its validation through in silico cloning and immune simulation
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/d0c671d475e144ab999527a760bdbdef
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Immunoinformatics and Immunogenetics-Based Design of Immunogenic Peptides Vaccine against the Emerging Tick-Borne Encephalitis Virus (TBEV) and Its Validation through In Silico Cloning and Immune Simulation

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