Detection of fluorescent organic nanoparticles by confocal laser endomicroscopy in a rat model of Barrett’s esophageal adenocarcinoma

Detection of fluorescent organic nanoparticles by confocal laser endomicroscopy in a rat model of Barrett’s esophageal adenocarcinoma

Elisa Dassie,1,2,* Diletta Arcidiacono,2,3,* Iga Wasiak,4 Nunzio Damiano,5 Luigi Dall’Olmo,6 Cinzia Giacometti,7 Sonia Facchin,3 Mauro Cassaro,7 Ennio Guido,8 Franca De Lazzari,8 Oriano Marin,9,10 Tomasz Ciach,4 Suzanne Fery-Forgues,11,12 Alfredo Alberti,1,2 Giorgio Battaglia,13 Stefano Re...

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Autores principales: Dassie E, Arcidiacono D, Wasiak I, Damiano N, Dall'Olmo L, Giacometti C, Facchin S, Cassaro M, Guido E, De Lazzari F, Marin O, Ciach T, Fery-Forgues S, Alberti A, Battaglia G, Realdon S
Formato: article
Lenguaje:EN
Publicado: Dove Medical Press 2015
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Medicine (General)
R5-920
Acceso en línea:https://doaj.org/article/d0c995e5a7104a8bbede44a95918d32b
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id oai:doaj.org-article:d0c995e5a7104a8bbede44a95918d32b
record_format dspace
institution DOAJ
collection DOAJ
language EN
topic Medicine (General)
R5-920
spellingShingle Medicine (General)
R5-920
Dassie E
Arcidiacono D
Wasiak I
Damiano N
Dall'Olmo L
Giacometti C
Facchin S
Cassaro M
Guido E
De Lazzari F
Marin O
Ciach T
Fery-Forgues S
Alberti A
Battaglia G
Realdon S
Detection of fluorescent organic nanoparticles by confocal laser endomicroscopy in a rat model of Barrett’s esophageal adenocarcinoma
description Elisa Dassie,1,2,* Diletta Arcidiacono,2,3,* Iga Wasiak,4 Nunzio Damiano,5 Luigi Dall’Olmo,6 Cinzia Giacometti,7 Sonia Facchin,3 Mauro Cassaro,7 Ennio Guido,8 Franca De Lazzari,8 Oriano Marin,9,10 Tomasz Ciach,4 Suzanne Fery-Forgues,11,12 Alfredo Alberti,1,2 Giorgio Battaglia,13 Stefano Realdon13 1Department of Molecular Medicine, University of Padua, 2Venetian Institute of Molecular Medicine, 3Department of Surgery, Oncology and Gastroenterology, University of Padua, Padua, Italy; 4Faculty of Chemical and Process Engineering, Warsaw University of Technology, Warsaw, Poland; 5Department of Biomedical Sciences, University of Padua, Padua, 6Department of Emergency Medicine, “Santi Giovanni e Paolo” Hospital, Venice, 7Anatomic Pathology Unit, ULSS 15, Alta Padovana, Camposampiero, 8Gastroenterology Unit, Sant’Antonio Hospital, 9Interdepartmental Research Centre for Innovative Biotechnologies (CRIBI), University of Padua, 10Proteomics Facility, Azienda Ospedaliera di Padova, Padua, 11CNRS, ITAV-USR 3505, Toulouse, France; 12Université de Toulouse, ITAV-USR 3505, Toulouse, France; 13Endoscopy Unit, Veneto Institute of Oncology (IOV-IRCCS), Padua, Italy *These authors contributed equally to this work Abstract: For many years, novel strategies for cancer detection and treatment using nanoparticles (NPs) have been developed. Esophageal adenocarcinoma is the sixth leading cause of cancer-related deaths in Western countries, and despite recent advances in early detection and treatment, its prognosis is still very poor. This study investigated the use of fluorescent organic NPs as potential diagnostic tool in an experimental in vivo model of Barrett’s esophageal adenocarcinoma. NPs were made of modified polysaccharides loaded with [4-(dicyanomethylene)-2-methyl-6-(4-dimethylaminostyryl)-4H-pyran] (DCM), a well-known fluorescent dye. The NP periphery might or might not be decorated with ASYNYDA peptide that has an affinity for esophageal cancer cells. Non-operated and operated rats in which gastroesophageal reflux was surgically induced received both types of NPs (NP-DCM and NP-DCM-ASYNYDA) by intravenous route. Localization of mucosal NPs was assessed in vivo by confocal laser endomicroscopy, a technique which enables a “real time” and in situ visualization of the tissue at a cellular level. After injection of NP-DCM and NP-DCM-ASYNYDA, fluorescence was observed in rats affected by esophageal cancer, whereas no signal was observed in control non-operated rats, or in rats with simple esophagitis or Barrett’s esophagus mucosa. Fluorescence was observable in vivo 30 minutes after the administration of NPs. Interestingly, NP-DCM-ASYNYDA induced strong fluorescence intensity 24 hours after administration. These observations suggested that NPs could reach the tumor cells, likely by enhanced permeability and retention effect, and the peptide ASYNYDA gave them high specificity for esophageal cancer cells. Thus, the combination of NP platform and confocal laser endomicroscopy could play an important role for highlighting esophageal cancer conditions. This result supports the potential of this strategy as a targeted carrier for photoactive and bioactive molecules in esophageal cancer diagnosis and treatment. Keywords: confocal laser endomicroscopy, Barrett’s esophagus, diagnostics, esophageal adenocarcinoma, fluorescent nanoparticles, heptapeptide
format article
author Dassie E
Arcidiacono D
Wasiak I
Damiano N
Dall'Olmo L
Giacometti C
Facchin S
Cassaro M
Guido E
De Lazzari F
Marin O
Ciach T
Fery-Forgues S
Alberti A
Battaglia G
Realdon S
author_facet Dassie E
Arcidiacono D
Wasiak I
Damiano N
Dall'Olmo L
Giacometti C
Facchin S
Cassaro M
Guido E
De Lazzari F
Marin O
Ciach T
Fery-Forgues S
Alberti A
Battaglia G
Realdon S
author_sort Dassie E
title Detection of fluorescent organic nanoparticles by confocal laser endomicroscopy in a rat model of Barrett’s esophageal adenocarcinoma
title_short Detection of fluorescent organic nanoparticles by confocal laser endomicroscopy in a rat model of Barrett’s esophageal adenocarcinoma
title_full Detection of fluorescent organic nanoparticles by confocal laser endomicroscopy in a rat model of Barrett’s esophageal adenocarcinoma
title_fullStr Detection of fluorescent organic nanoparticles by confocal laser endomicroscopy in a rat model of Barrett’s esophageal adenocarcinoma
title_full_unstemmed Detection of fluorescent organic nanoparticles by confocal laser endomicroscopy in a rat model of Barrett’s esophageal adenocarcinoma
title_sort detection of fluorescent organic nanoparticles by confocal laser endomicroscopy in a rat model of barrett’s esophageal adenocarcinoma
publisher Dove Medical Press
publishDate 2015
url https://doaj.org/article/d0c995e5a7104a8bbede44a95918d32b
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spelling oai:doaj.org-article:d0c995e5a7104a8bbede44a95918d32b2021-12-02T02:11:14ZDetection of fluorescent organic nanoparticles by confocal laser endomicroscopy in a rat model of Barrett’s esophageal adenocarcinoma1178-2013https://doaj.org/article/d0c995e5a7104a8bbede44a95918d32b2015-10-01T00:00:00Zhttps://www.dovepress.com/detection-of-fluorescent-organic-nanoparticles-bynbspconfocal-laser-en-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Elisa Dassie,1,2,* Diletta Arcidiacono,2,3,* Iga Wasiak,4 Nunzio Damiano,5 Luigi Dall’Olmo,6 Cinzia Giacometti,7 Sonia Facchin,3 Mauro Cassaro,7 Ennio Guido,8 Franca De Lazzari,8 Oriano Marin,9,10 Tomasz Ciach,4 Suzanne Fery-Forgues,11,12 Alfredo Alberti,1,2 Giorgio Battaglia,13 Stefano Realdon13 1Department of Molecular Medicine, University of Padua, 2Venetian Institute of Molecular Medicine, 3Department of Surgery, Oncology and Gastroenterology, University of Padua, Padua, Italy; 4Faculty of Chemical and Process Engineering, Warsaw University of Technology, Warsaw, Poland; 5Department of Biomedical Sciences, University of Padua, Padua, 6Department of Emergency Medicine, “Santi Giovanni e Paolo” Hospital, Venice, 7Anatomic Pathology Unit, ULSS 15, Alta Padovana, Camposampiero, 8Gastroenterology Unit, Sant’Antonio Hospital, 9Interdepartmental Research Centre for Innovative Biotechnologies (CRIBI), University of Padua, 10Proteomics Facility, Azienda Ospedaliera di Padova, Padua, 11CNRS, ITAV-USR 3505, Toulouse, France; 12Université de Toulouse, ITAV-USR 3505, Toulouse, France; 13Endoscopy Unit, Veneto Institute of Oncology (IOV-IRCCS), Padua, Italy *These authors contributed equally to this work Abstract: For many years, novel strategies for cancer detection and treatment using nanoparticles (NPs) have been developed. Esophageal adenocarcinoma is the sixth leading cause of cancer-related deaths in Western countries, and despite recent advances in early detection and treatment, its prognosis is still very poor. This study investigated the use of fluorescent organic NPs as potential diagnostic tool in an experimental in vivo model of Barrett’s esophageal adenocarcinoma. NPs were made of modified polysaccharides loaded with [4-(dicyanomethylene)-2-methyl-6-(4-dimethylaminostyryl)-4H-pyran] (DCM), a well-known fluorescent dye. The NP periphery might or might not be decorated with ASYNYDA peptide that has an affinity for esophageal cancer cells. Non-operated and operated rats in which gastroesophageal reflux was surgically induced received both types of NPs (NP-DCM and NP-DCM-ASYNYDA) by intravenous route. Localization of mucosal NPs was assessed in vivo by confocal laser endomicroscopy, a technique which enables a “real time” and in situ visualization of the tissue at a cellular level. After injection of NP-DCM and NP-DCM-ASYNYDA, fluorescence was observed in rats affected by esophageal cancer, whereas no signal was observed in control non-operated rats, or in rats with simple esophagitis or Barrett’s esophagus mucosa. Fluorescence was observable in vivo 30 minutes after the administration of NPs. Interestingly, NP-DCM-ASYNYDA induced strong fluorescence intensity 24 hours after administration. These observations suggested that NPs could reach the tumor cells, likely by enhanced permeability and retention effect, and the peptide ASYNYDA gave them high specificity for esophageal cancer cells. Thus, the combination of NP platform and confocal laser endomicroscopy could play an important role for highlighting esophageal cancer conditions. This result supports the potential of this strategy as a targeted carrier for photoactive and bioactive molecules in esophageal cancer diagnosis and treatment. Keywords: confocal laser endomicroscopy, Barrett’s esophagus, diagnostics, esophageal adenocarcinoma, fluorescent nanoparticles, heptapeptideDassie EArcidiacono DWasiak IDamiano NDall'Olmo LGiacometti CFacchin SCassaro MGuido EDe Lazzari FMarin OCiach TFery-Forgues SAlberti ABattaglia GRealdon SDove Medical PressarticleMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2015, Iss default, Pp 6811-6823 (2015)

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