Genotoxicity of physical silver nanoparticles, produced by the HVAD method, for Chinchilla lanigera genome

Abstract Each year, growing demand for silver nanoparticles (AgNP) contributes to the search for alternative methods of their production. Stable AgNP with antibacterial properties, low toxicity to the environment and living organisms are especially valued. In the study presented here, an attempt was...

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Autores principales:	Anna Grzesiakowska, Marek Jan Kasprowicz, Marta Kuchta-Gładysz, Katarzyna Rymuza, Olga Szeleszczuk
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/d0d200273f274feaa474238560ea6fd1
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spelling	oai:doaj.org-article:d0d200273f274feaa474238560ea6fd12021-12-02T17:24:11ZGenotoxicity of physical silver nanoparticles, produced by the HVAD method, for Chinchilla lanigera genome10.1038/s41598-021-97926-92045-2322https://doaj.org/article/d0d200273f274feaa474238560ea6fd12021-09-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-97926-9https://doaj.org/toc/2045-2322Abstract Each year, growing demand for silver nanoparticles (AgNP) contributes to the search for alternative methods of their production. Stable AgNP with antibacterial properties, low toxicity to the environment and living organisms are especially valued. In the study presented here, an attempt was made to assess the toxicity of two AgNP solutions produced using the HVAD method to the Chinchilla lanigera genome. The AgNO3 solution was the indicator and reference for the harmfulness of AgNP. The study was carried out in vitro on bone marrow cells isolated from Chinchilla lanigera bones. The genotoxicity was assessed by comet assay, following the treatment of cells with three silver solutions: unstable and sodium citrate-stabilized silver nanoparticles, as well as silver nitrate at three concentrations (5, 10 and 20 µg/L), after 3, 6 and 24 h. Based on the percentage of the DNA content in the comet tail and the tail moment, an increase in cell DNA integrity disruption was demonstrated in all tested variants: of solution, exposure time and concentration, compared to the control sample. A statistically significant correlation was determined between the level of induced DNA breaks and the concentration of the active solutions and the duration of their activity. A solution of silver nanoparticles stabilized with sodium citrate was shown to have the most harmful effect on bone marrow cells. Silver nitrate demonstrated a level of toxicity similar to these particles. Further studies are necessary to directly compare the genotoxic properties of AgNP produced using the HVAD method and the chemical method under the same conditions.Anna GrzesiakowskaMarek Jan KasprowiczMarta Kuchta-GładyszKatarzyna RymuzaOlga SzeleszczukNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-9 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Anna Grzesiakowska Marek Jan Kasprowicz Marta Kuchta-Gładysz Katarzyna Rymuza Olga Szeleszczuk Genotoxicity of physical silver nanoparticles, produced by the HVAD method, for Chinchilla lanigera genome
description	Abstract Each year, growing demand for silver nanoparticles (AgNP) contributes to the search for alternative methods of their production. Stable AgNP with antibacterial properties, low toxicity to the environment and living organisms are especially valued. In the study presented here, an attempt was made to assess the toxicity of two AgNP solutions produced using the HVAD method to the Chinchilla lanigera genome. The AgNO3 solution was the indicator and reference for the harmfulness of AgNP. The study was carried out in vitro on bone marrow cells isolated from Chinchilla lanigera bones. The genotoxicity was assessed by comet assay, following the treatment of cells with three silver solutions: unstable and sodium citrate-stabilized silver nanoparticles, as well as silver nitrate at three concentrations (5, 10 and 20 µg/L), after 3, 6 and 24 h. Based on the percentage of the DNA content in the comet tail and the tail moment, an increase in cell DNA integrity disruption was demonstrated in all tested variants: of solution, exposure time and concentration, compared to the control sample. A statistically significant correlation was determined between the level of induced DNA breaks and the concentration of the active solutions and the duration of their activity. A solution of silver nanoparticles stabilized with sodium citrate was shown to have the most harmful effect on bone marrow cells. Silver nitrate demonstrated a level of toxicity similar to these particles. Further studies are necessary to directly compare the genotoxic properties of AgNP produced using the HVAD method and the chemical method under the same conditions.
format	article
author	Anna Grzesiakowska Marek Jan Kasprowicz Marta Kuchta-Gładysz Katarzyna Rymuza Olga Szeleszczuk
author_facet	Anna Grzesiakowska Marek Jan Kasprowicz Marta Kuchta-Gładysz Katarzyna Rymuza Olga Szeleszczuk
author_sort	Anna Grzesiakowska
title	Genotoxicity of physical silver nanoparticles, produced by the HVAD method, for Chinchilla lanigera genome
title_short	Genotoxicity of physical silver nanoparticles, produced by the HVAD method, for Chinchilla lanigera genome
title_full	Genotoxicity of physical silver nanoparticles, produced by the HVAD method, for Chinchilla lanigera genome
title_fullStr	Genotoxicity of physical silver nanoparticles, produced by the HVAD method, for Chinchilla lanigera genome
title_full_unstemmed	Genotoxicity of physical silver nanoparticles, produced by the HVAD method, for Chinchilla lanigera genome
title_sort	genotoxicity of physical silver nanoparticles, produced by the hvad method, for chinchilla lanigera genome
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/d0d200273f274feaa474238560ea6fd1
work_keys_str_mv	AT annagrzesiakowska genotoxicityofphysicalsilvernanoparticlesproducedbythehvadmethodforchinchillalanigeragenome AT marekjankasprowicz genotoxicityofphysicalsilvernanoparticlesproducedbythehvadmethodforchinchillalanigeragenome AT martakuchtagładysz genotoxicityofphysicalsilvernanoparticlesproducedbythehvadmethodforchinchillalanigeragenome AT katarzynarymuza genotoxicityofphysicalsilvernanoparticlesproducedbythehvadmethodforchinchillalanigeragenome AT olgaszeleszczuk genotoxicityofphysicalsilvernanoparticlesproducedbythehvadmethodforchinchillalanigeragenome
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Genotoxicity of physical silver nanoparticles, produced by the HVAD method, for Chinchilla lanigera genome

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