Mid-gestation cytokine profiles in mothers of children affected by autism spectrum disorder: a case–control study

Abstract Autism Spectrum disorder is one of the commonest and most important neurodevelopmental conditions affecting children today. With an increasing prevalence and an unclear aetiology, it is imperative we find early markers of autism, which may facilitate early identification and intervention. A...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Michael Carter, Sophie Casey, Gerard W. O’Keeffe, Louise Gibson, Deirdre M. Murray
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/d0d8fd162d8c422f92290dc48567a0c7
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:d0d8fd162d8c422f92290dc48567a0c7
record_format	dspace
spelling	oai:doaj.org-article:d0d8fd162d8c422f92290dc48567a0c72021-11-21T12:16:22ZMid-gestation cytokine profiles in mothers of children affected by autism spectrum disorder: a case–control study10.1038/s41598-021-01662-z2045-2322https://doaj.org/article/d0d8fd162d8c422f92290dc48567a0c72021-11-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-01662-zhttps://doaj.org/toc/2045-2322Abstract Autism Spectrum disorder is one of the commonest and most important neurodevelopmental conditions affecting children today. With an increasing prevalence and an unclear aetiology, it is imperative we find early markers of autism, which may facilitate early identification and intervention. Alterations of gestational cytokine profiles have been reported in mothers of autistic children. Increasing evidence suggests that the intrauterine environment is an important determinant of autism risk. This study aims to examine the mid-gestational serum cytokine profiles of the mothers of autistic children from a well-characterised birth cohort. A nested sub-cohort within a large mother–child birth cohort were identified based on a confirmed multi-disciplinary diagnosis of autism before the age 10 years and neuro-typical matched controls in a 2:1 ratio. IFN-γ, IL-1β, IL-4, IL-6, IL-8, IL-17A, GMCSF and TNFα were measured in archived maternal 20-week serum using MesoScale Diagnostics multiplex technology and validation of our IL-17A measurements was performed using an ultrasensitive assay. From a cohort of 2137 children, 25 had confirmed autism before 10 years and stored maternal serum from mid-gestation. We examined the sera of these 25 cases and 50 matched controls. The sex ratio was 4:1 males to females in each group, and the mean age at diagnosis was 5.09 years (SD 2.13). We found that concentrations of IL-4 were significantly altered between groups. The other analytes did not differ significantly using either multiplex or ultra-sensitive assays. In our well-characterised prospective cohort of autistic children, we confirmed mid-gestational alterations in maternal IL-4 concentrations in autism affected pregnancies versus matched controls. These findings add to promising evidence from animal models and retrospective screening programmes and adds to the knowledge in this field.Michael CarterSophie CaseyGerard W. O’KeeffeLouise GibsonDeirdre M. MurrayNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-17 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Michael Carter Sophie Casey Gerard W. O’Keeffe Louise Gibson Deirdre M. Murray Mid-gestation cytokine profiles in mothers of children affected by autism spectrum disorder: a case–control study
description	Abstract Autism Spectrum disorder is one of the commonest and most important neurodevelopmental conditions affecting children today. With an increasing prevalence and an unclear aetiology, it is imperative we find early markers of autism, which may facilitate early identification and intervention. Alterations of gestational cytokine profiles have been reported in mothers of autistic children. Increasing evidence suggests that the intrauterine environment is an important determinant of autism risk. This study aims to examine the mid-gestational serum cytokine profiles of the mothers of autistic children from a well-characterised birth cohort. A nested sub-cohort within a large mother–child birth cohort were identified based on a confirmed multi-disciplinary diagnosis of autism before the age 10 years and neuro-typical matched controls in a 2:1 ratio. IFN-γ, IL-1β, IL-4, IL-6, IL-8, IL-17A, GMCSF and TNFα were measured in archived maternal 20-week serum using MesoScale Diagnostics multiplex technology and validation of our IL-17A measurements was performed using an ultrasensitive assay. From a cohort of 2137 children, 25 had confirmed autism before 10 years and stored maternal serum from mid-gestation. We examined the sera of these 25 cases and 50 matched controls. The sex ratio was 4:1 males to females in each group, and the mean age at diagnosis was 5.09 years (SD 2.13). We found that concentrations of IL-4 were significantly altered between groups. The other analytes did not differ significantly using either multiplex or ultra-sensitive assays. In our well-characterised prospective cohort of autistic children, we confirmed mid-gestational alterations in maternal IL-4 concentrations in autism affected pregnancies versus matched controls. These findings add to promising evidence from animal models and retrospective screening programmes and adds to the knowledge in this field.
format	article
author	Michael Carter Sophie Casey Gerard W. O’Keeffe Louise Gibson Deirdre M. Murray
author_facet	Michael Carter Sophie Casey Gerard W. O’Keeffe Louise Gibson Deirdre M. Murray
author_sort	Michael Carter
title	Mid-gestation cytokine profiles in mothers of children affected by autism spectrum disorder: a case–control study
title_short	Mid-gestation cytokine profiles in mothers of children affected by autism spectrum disorder: a case–control study
title_full	Mid-gestation cytokine profiles in mothers of children affected by autism spectrum disorder: a case–control study
title_fullStr	Mid-gestation cytokine profiles in mothers of children affected by autism spectrum disorder: a case–control study
title_full_unstemmed	Mid-gestation cytokine profiles in mothers of children affected by autism spectrum disorder: a case–control study
title_sort	mid-gestation cytokine profiles in mothers of children affected by autism spectrum disorder: a case–control study
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/d0d8fd162d8c422f92290dc48567a0c7
work_keys_str_mv	AT michaelcarter midgestationcytokineprofilesinmothersofchildrenaffectedbyautismspectrumdisorderacasecontrolstudy AT sophiecasey midgestationcytokineprofilesinmothersofchildrenaffectedbyautismspectrumdisorderacasecontrolstudy AT gerardwokeeffe midgestationcytokineprofilesinmothersofchildrenaffectedbyautismspectrumdisorderacasecontrolstudy AT louisegibson midgestationcytokineprofilesinmothersofchildrenaffectedbyautismspectrumdisorderacasecontrolstudy AT deirdremmurray midgestationcytokineprofilesinmothersofchildrenaffectedbyautismspectrumdisorderacasecontrolstudy
_version_	1718419151484092416

Mid-gestation cytokine profiles in mothers of children affected by autism spectrum disorder: a case–control study

Ejemplares similares