Comparison of gene expression in the red imported fire ant (Solenopsis invicta) under different temperature conditions

Abstract The red imported fire ant (RIFA), Solenopsis invicta Buren is native to South America and is known as a global problematic invasive species. This study focused on the molecular response of RIFA by comparing gene expression profiles after exposing ants to low (10 °C) and high (40 °C) tempera...

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Autores principales:	Mohammad Vatanparast, Robert T. Puckett, Deuk-Soo Choi, Youngjin Park
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/d0db14d50b2945a9969f93bb672b6ed5
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spelling	oai:doaj.org-article:d0db14d50b2945a9969f93bb672b6ed52021-12-02T16:43:39ZComparison of gene expression in the red imported fire ant (Solenopsis invicta) under different temperature conditions10.1038/s41598-021-95779-w2045-2322https://doaj.org/article/d0db14d50b2945a9969f93bb672b6ed52021-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-95779-whttps://doaj.org/toc/2045-2322Abstract The red imported fire ant (RIFA), Solenopsis invicta Buren is native to South America and is known as a global problematic invasive species. This study focused on the molecular response of RIFA by comparing gene expression profiles after exposing ants to low (10 °C) and high (40 °C) temperature stress and comparing them to untreated controls (30 °C). A total of 99,085 unigenes (the clustered non-redundant transcripts that are filtered from the longest assembled contigs) were obtained, of which 19,154 were annotated with gene descriptions, gene ontology terms, and metabolic pathways. 86 gene ontology (GO) functional sub-groups and 23 EggNOG terms resulted. Differentially expressed genes (DEGs) with log2FC ≥ 10 were screened and were compared at different temperatures. We found 203, 48, and 66 specific DEGs co-regulated at 10, 20, and 40 °C. Comparing transcriptome profiles for differential gene expression resulted in various DE genes, including cytochrome P450, NADH dehydrogenase subunit 1, cuticle protein and heat shock protein (HSP), which have previously been reported to be involved in cold and high temperature resistance. GO analysis revealed that antioxidant activity is up-regulated under high temperature stress. We verified the RNA-seq data by qPCR on 20 up- and down-regulated DEGs. These findings provide a basis for future understanding of the adaptation mechanisms of RIFA and the molecular mechanisms underlying the response to low and high temperatures.Mohammad VatanparastRobert T. PuckettDeuk-Soo ChoiYoungjin ParkNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-14 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Mohammad Vatanparast Robert T. Puckett Deuk-Soo Choi Youngjin Park Comparison of gene expression in the red imported fire ant (Solenopsis invicta) under different temperature conditions
description	Abstract The red imported fire ant (RIFA), Solenopsis invicta Buren is native to South America and is known as a global problematic invasive species. This study focused on the molecular response of RIFA by comparing gene expression profiles after exposing ants to low (10 °C) and high (40 °C) temperature stress and comparing them to untreated controls (30 °C). A total of 99,085 unigenes (the clustered non-redundant transcripts that are filtered from the longest assembled contigs) were obtained, of which 19,154 were annotated with gene descriptions, gene ontology terms, and metabolic pathways. 86 gene ontology (GO) functional sub-groups and 23 EggNOG terms resulted. Differentially expressed genes (DEGs) with log2FC ≥ 10 were screened and were compared at different temperatures. We found 203, 48, and 66 specific DEGs co-regulated at 10, 20, and 40 °C. Comparing transcriptome profiles for differential gene expression resulted in various DE genes, including cytochrome P450, NADH dehydrogenase subunit 1, cuticle protein and heat shock protein (HSP), which have previously been reported to be involved in cold and high temperature resistance. GO analysis revealed that antioxidant activity is up-regulated under high temperature stress. We verified the RNA-seq data by qPCR on 20 up- and down-regulated DEGs. These findings provide a basis for future understanding of the adaptation mechanisms of RIFA and the molecular mechanisms underlying the response to low and high temperatures.
format	article
author	Mohammad Vatanparast Robert T. Puckett Deuk-Soo Choi Youngjin Park
author_facet	Mohammad Vatanparast Robert T. Puckett Deuk-Soo Choi Youngjin Park
author_sort	Mohammad Vatanparast
title	Comparison of gene expression in the red imported fire ant (Solenopsis invicta) under different temperature conditions
title_short	Comparison of gene expression in the red imported fire ant (Solenopsis invicta) under different temperature conditions
title_full	Comparison of gene expression in the red imported fire ant (Solenopsis invicta) under different temperature conditions
title_fullStr	Comparison of gene expression in the red imported fire ant (Solenopsis invicta) under different temperature conditions
title_full_unstemmed	Comparison of gene expression in the red imported fire ant (Solenopsis invicta) under different temperature conditions
title_sort	comparison of gene expression in the red imported fire ant (solenopsis invicta) under different temperature conditions
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/d0db14d50b2945a9969f93bb672b6ed5
work_keys_str_mv	AT mohammadvatanparast comparisonofgeneexpressionintheredimportedfireantsolenopsisinvictaunderdifferenttemperatureconditions AT roberttpuckett comparisonofgeneexpressionintheredimportedfireantsolenopsisinvictaunderdifferenttemperatureconditions AT deuksoochoi comparisonofgeneexpressionintheredimportedfireantsolenopsisinvictaunderdifferenttemperatureconditions AT youngjinpark comparisonofgeneexpressionintheredimportedfireantsolenopsisinvictaunderdifferenttemperatureconditions
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Comparison of gene expression in the red imported fire ant (Solenopsis invicta) under different temperature conditions

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