The broad role of Nkx3.2 in the development of the zebrafish axial skeleton.
The transcription factor Nkx3.2 (Bapx1) is an important chondrocyte maturation inhibitor. Previous Nkx3.2 knockdown and overexpression studies in non-mammalian gnathostomes have focused on its role in primary jaw joint development, while the function of this gene in broader skeletal development is n...
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2021
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oai:doaj.org-article:d0dc2d373c894c62afa916c413eb7f8e2021-12-02T20:17:45ZThe broad role of Nkx3.2 in the development of the zebrafish axial skeleton.1932-620310.1371/journal.pone.0255953https://doaj.org/article/d0dc2d373c894c62afa916c413eb7f8e2021-01-01T00:00:00Zhttps://doi.org/10.1371/journal.pone.0255953https://doaj.org/toc/1932-6203The transcription factor Nkx3.2 (Bapx1) is an important chondrocyte maturation inhibitor. Previous Nkx3.2 knockdown and overexpression studies in non-mammalian gnathostomes have focused on its role in primary jaw joint development, while the function of this gene in broader skeletal development is not fully described. We generated a mutant allele of nkx3.2 in zebrafish with CRISPR/Cas9 and applied a range of techniques to characterize skeletal phenotypes at developmental stages from larva to adult, revealing loss of the jaw joint, fusions in bones of the occiput, morphological changes in the Weberian apparatus, and the loss or deformation of bony elements derived from basiventral cartilages of the vertebrae. Axial phenotypes are reminiscent of Nkx3.2 knockout in mammals, suggesting that the function of this gene in axial skeletal development is ancestral to osteichthyans. Our results highlight the broad role of nkx3.2 in zebrafish skeletal development and its context-specific functions in different skeletal elements.Laura WaldmannJake LeyhrHanqing ZhangCaroline Öhman-MägiAmin AllalouTatjana HaitinaPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 16, Iss 8, p e0255953 (2021) |
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Medicine R Science Q |
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Medicine R Science Q Laura Waldmann Jake Leyhr Hanqing Zhang Caroline Öhman-Mägi Amin Allalou Tatjana Haitina The broad role of Nkx3.2 in the development of the zebrafish axial skeleton. |
| description |
The transcription factor Nkx3.2 (Bapx1) is an important chondrocyte maturation inhibitor. Previous Nkx3.2 knockdown and overexpression studies in non-mammalian gnathostomes have focused on its role in primary jaw joint development, while the function of this gene in broader skeletal development is not fully described. We generated a mutant allele of nkx3.2 in zebrafish with CRISPR/Cas9 and applied a range of techniques to characterize skeletal phenotypes at developmental stages from larva to adult, revealing loss of the jaw joint, fusions in bones of the occiput, morphological changes in the Weberian apparatus, and the loss or deformation of bony elements derived from basiventral cartilages of the vertebrae. Axial phenotypes are reminiscent of Nkx3.2 knockout in mammals, suggesting that the function of this gene in axial skeletal development is ancestral to osteichthyans. Our results highlight the broad role of nkx3.2 in zebrafish skeletal development and its context-specific functions in different skeletal elements. |
| format |
article |
| author |
Laura Waldmann Jake Leyhr Hanqing Zhang Caroline Öhman-Mägi Amin Allalou Tatjana Haitina |
| author_facet |
Laura Waldmann Jake Leyhr Hanqing Zhang Caroline Öhman-Mägi Amin Allalou Tatjana Haitina |
| author_sort |
Laura Waldmann |
| title |
The broad role of Nkx3.2 in the development of the zebrafish axial skeleton. |
| title_short |
The broad role of Nkx3.2 in the development of the zebrafish axial skeleton. |
| title_full |
The broad role of Nkx3.2 in the development of the zebrafish axial skeleton. |
| title_fullStr |
The broad role of Nkx3.2 in the development of the zebrafish axial skeleton. |
| title_full_unstemmed |
The broad role of Nkx3.2 in the development of the zebrafish axial skeleton. |
| title_sort |
broad role of nkx3.2 in the development of the zebrafish axial skeleton. |
| publisher |
Public Library of Science (PLoS) |
| publishDate |
2021 |
| url |
https://doaj.org/article/d0dc2d373c894c62afa916c413eb7f8e |
| work_keys_str_mv |
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