Identification of pigment profiles and antioxidant activity of Rhizophora mucronata mangrove leaves origin Lembeh, North Sulawesi, Indonesia

Abstract. Rumengan AP, Mandiangan ES, Tanod WA, Paransa DSJ, Paruntu CP, Mantiri DMH. 2021. Identification of pigment profiles and antioxidant activity of Rhizophora mucronata mangrove leaves origin Lembeh, North Sulawesi, Indonesia. Biodiversitas 22: 2805-2816. Mangroves plants contain unique pigme...

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Autores principales:	ANTONIUS PETRUS RUMENGAN, ELVI SISKA MANDIANGAN, Wendy Alexander Tanod, DARUS SAADAH JOHANIS PARANSA, CAROLUS PAULUS PARUNTU, DESY MARIA HELENA MANTIRI
Formato:	article
Lenguaje:	EN
Publicado:	MBI & UNS Solo 2021
Materias:	beta-carotene, chlorophyll, lutein, neoxanthin, violaxanthin Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/d0dc7a7b8a294ec29680f1dccb15063e
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spelling	oai:doaj.org-article:d0dc7a7b8a294ec29680f1dccb15063e2021-11-22T12:16:55ZIdentification of pigment profiles and antioxidant activity of Rhizophora mucronata mangrove leaves origin Lembeh, North Sulawesi, Indonesia1412-033X2085-472210.13057/biodiv/d220730https://doaj.org/article/d0dc7a7b8a294ec29680f1dccb15063e2021-06-01T00:00:00Zhttps://smujo.id/biodiv/article/view/8632https://doaj.org/toc/1412-033Xhttps://doaj.org/toc/2085-4722Abstract. Rumengan AP, Mandiangan ES, Tanod WA, Paransa DSJ, Paruntu CP, Mantiri DMH. 2021. Identification of pigment profiles and antioxidant activity of Rhizophora mucronata mangrove leaves origin Lembeh, North Sulawesi, Indonesia. Biodiversitas 22: 2805-2816. Mangroves plants contain unique pigments which can serve both nutraceutical and pharmaceutical purpose. Therefore, this preliminary study aims to identify the pigment profiles of Rhizophora mucronata mangrove leaves (HPLC method) and to computationally evaluate the antioxidant mechanism of the pigments (PASS and STITCH analysis). Furthermore, it evaluated the antioxidant capacity of R. mucronata leaves extracts (DPPH method), and the pigment profiles detected using HPLC were chlorophyll a (68.61%), chlorophyll b (27.69%), lutein (29.94%), beta-carotene (14.05%), pheophytin a (8.72), violaxanthin (5.19%), and neoxanthin (3.65%). Beta-carotene, lutein, neoxanthin, and violaxanthin were predicted to have potential as antioxidants properties using PASS analysis. While neoxanthin and violaxanthin were predicted as free radical scavengers, beta-carotene was an Nrf-2 stimulant. The STITCH analysis showed that the pigments contained in the leaves interacted synergistically by activating as antioxidant enzymes and inhibiting the expression of oxidative stress proteins. The ethanol extract of R. mucronata leaves may be a potent antioxidant with an IC50 20.99 ± 0.33 µg/mL. Therefore, the pigment contained in R. mucronata leaves is a potential source of antioxidants.ANTONIUS PETRUS RUMENGANELVI SISKA MANDIANGANWendy Alexander TanodDARUS SAADAH JOHANIS PARANSACAROLUS PAULUS PARUNTUDESY MARIA HELENA MANTIRIMBI & UNS Soloarticlebeta-carotene, chlorophyll, lutein, neoxanthin, violaxanthinBiology (General)QH301-705.5ENBiodiversitas, Vol 22, Iss 7 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	beta-carotene, chlorophyll, lutein, neoxanthin, violaxanthin Biology (General) QH301-705.5
spellingShingle	beta-carotene, chlorophyll, lutein, neoxanthin, violaxanthin Biology (General) QH301-705.5 ANTONIUS PETRUS RUMENGAN ELVI SISKA MANDIANGAN Wendy Alexander Tanod DARUS SAADAH JOHANIS PARANSA CAROLUS PAULUS PARUNTU DESY MARIA HELENA MANTIRI Identification of pigment profiles and antioxidant activity of Rhizophora mucronata mangrove leaves origin Lembeh, North Sulawesi, Indonesia
description	Abstract. Rumengan AP, Mandiangan ES, Tanod WA, Paransa DSJ, Paruntu CP, Mantiri DMH. 2021. Identification of pigment profiles and antioxidant activity of Rhizophora mucronata mangrove leaves origin Lembeh, North Sulawesi, Indonesia. Biodiversitas 22: 2805-2816. Mangroves plants contain unique pigments which can serve both nutraceutical and pharmaceutical purpose. Therefore, this preliminary study aims to identify the pigment profiles of Rhizophora mucronata mangrove leaves (HPLC method) and to computationally evaluate the antioxidant mechanism of the pigments (PASS and STITCH analysis). Furthermore, it evaluated the antioxidant capacity of R. mucronata leaves extracts (DPPH method), and the pigment profiles detected using HPLC were chlorophyll a (68.61%), chlorophyll b (27.69%), lutein (29.94%), beta-carotene (14.05%), pheophytin a (8.72), violaxanthin (5.19%), and neoxanthin (3.65%). Beta-carotene, lutein, neoxanthin, and violaxanthin were predicted to have potential as antioxidants properties using PASS analysis. While neoxanthin and violaxanthin were predicted as free radical scavengers, beta-carotene was an Nrf-2 stimulant. The STITCH analysis showed that the pigments contained in the leaves interacted synergistically by activating as antioxidant enzymes and inhibiting the expression of oxidative stress proteins. The ethanol extract of R. mucronata leaves may be a potent antioxidant with an IC50 20.99 ± 0.33 µg/mL. Therefore, the pigment contained in R. mucronata leaves is a potential source of antioxidants.
format	article
author	ANTONIUS PETRUS RUMENGAN ELVI SISKA MANDIANGAN Wendy Alexander Tanod DARUS SAADAH JOHANIS PARANSA CAROLUS PAULUS PARUNTU DESY MARIA HELENA MANTIRI
author_facet	ANTONIUS PETRUS RUMENGAN ELVI SISKA MANDIANGAN Wendy Alexander Tanod DARUS SAADAH JOHANIS PARANSA CAROLUS PAULUS PARUNTU DESY MARIA HELENA MANTIRI
author_sort	ANTONIUS PETRUS RUMENGAN
title	Identification of pigment profiles and antioxidant activity of Rhizophora mucronata mangrove leaves origin Lembeh, North Sulawesi, Indonesia
title_short	Identification of pigment profiles and antioxidant activity of Rhizophora mucronata mangrove leaves origin Lembeh, North Sulawesi, Indonesia
title_full	Identification of pigment profiles and antioxidant activity of Rhizophora mucronata mangrove leaves origin Lembeh, North Sulawesi, Indonesia
title_fullStr	Identification of pigment profiles and antioxidant activity of Rhizophora mucronata mangrove leaves origin Lembeh, North Sulawesi, Indonesia
title_full_unstemmed	Identification of pigment profiles and antioxidant activity of Rhizophora mucronata mangrove leaves origin Lembeh, North Sulawesi, Indonesia
title_sort	identification of pigment profiles and antioxidant activity of rhizophora mucronata mangrove leaves origin lembeh, north sulawesi, indonesia
publisher	MBI & UNS Solo
publishDate	2021
url	https://doaj.org/article/d0dc7a7b8a294ec29680f1dccb15063e
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Identification of pigment profiles and antioxidant activity of Rhizophora mucronata mangrove leaves origin Lembeh, North Sulawesi, Indonesia

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