Microbial consortium mediated growth promotion and Arsenic reduction in Rice: An integrated transcriptome and proteome profiling

The adverse effects of arsenic (As) contamination are well known. Rice is a staple food for 50% of the world population but the accumulation of As into rice hampers the food security and safety. Thus the amelioration of As stress and reduction of As levels in rice are needed. In this study, transcri...

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Autores principales:	Surabhi Awasthi, Reshu Chauhan, Yuvraj Indoliya, Abhishek Singh Chauhan, ShashankKumar Mishra, Lalit Agrawal, Sanjay Dwivedi, Shiv Naresh Singh, Suchi Srivastava, Poonam C. Singh, Puneet Singh Chauhan, Debasis Chakrabarty, Sudhakar Srivastava, Rudra Deo Tripathi
Formato:	article
Lenguaje:	EN
Publicado:	Elsevier 2021
Materias:	Chlorella vulgaris Omics approaches Pseudomonas putida Rice Stress amelioration Environmental pollution TD172-193.5 Environmental sciences GE1-350
Acceso en línea:	https://doaj.org/article/d0e2e154d80c46bca663c3d20464f1c1
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spelling	oai:doaj.org-article:d0e2e154d80c46bca663c3d20464f1c12021-12-04T04:32:00ZMicrobial consortium mediated growth promotion and Arsenic reduction in Rice: An integrated transcriptome and proteome profiling0147-651310.1016/j.ecoenv.2021.113004https://doaj.org/article/d0e2e154d80c46bca663c3d20464f1c12021-12-01T00:00:00Zhttp://www.sciencedirect.com/science/article/pii/S0147651321011167https://doaj.org/toc/0147-6513The adverse effects of arsenic (As) contamination are well known. Rice is a staple food for 50% of the world population but the accumulation of As into rice hampers the food security and safety. Thus the amelioration of As stress and reduction of As levels in rice are needed. In this study, transcriptome (Illumina sequencing) and proteome (2D gel electrophoresis) explored mechanisms of consortium (P. putida+C. vulgaris) mediated growth promotion and As amelioration in rice. The rice seedlings grown hydroponically in the Hewitt nutrient medium and after acclimatization, exposed to 50 µM As alone as well as with microbial consortium to observe the impact at morphological and molecular level. The inoculation of microbial consortium significantly ameliorated the As toxicity, improved growth of root hairs and maintained cellular integrity of the epidermis, exodermis and the stele region during As exposure. Several genes showed differential expression in As and As+P. putida. Down-regulation of As transporters (OsPIP2;2 and OsPIP2;3, OsTIP2;1) and higher expression of WRKY gene (WRKY28) during As+P. putida+C.vulgaris suggested reduction of As uptake in rice. The up-regulation of nutrient elements transporters (OsZIFL9, OsZIFL5, OsZIFL12 and OsZIP2, OsYSL15 and OsCOPT6) in the presence of consortium indicated the improved nutrient status of rice. Higher expression of regulatory elements like auxin/indole 3 acetic acid (AUX/IAA), WRKY and myeloblastosis (MYB) TFs and down-regulation of defense responsive genes such Glutathione-S-transferase, Peroxidase and Glutaredoxinduring As+P. putida+C.vulgaris exposure was also observed. Proteome profiling demonstrated differential abundance of proteins involved in photosynthesis (chlorophyll a/b binding protein, photosystem I Fe-S centre), energy metabolism (ATP synthase subunit beta) transport, signaling (tubulin 1, actin 1), defense (glutathione S-transferase, phenylalanine ammonia lyase) and amino acid metabolism (cysteine synthase, glutamine synthetase), which supported the As ameliorative and growth-promoting potential of microbial consortium during As stress in rice plants. The study gives comprehensive information about gene and protein changes in rice plants in As+consortium exposure.Surabhi AwasthiReshu ChauhanYuvraj IndoliyaAbhishek Singh ChauhanShashankKumar MishraLalit AgrawalSanjay DwivediShiv Naresh SinghSuchi SrivastavaPoonam C. SinghPuneet Singh ChauhanDebasis ChakrabartySudhakar SrivastavaRudra Deo TripathiElsevierarticleChlorella vulgarisOmics approachesPseudomonas putidaRiceStress ameliorationEnvironmental pollutionTD172-193.5Environmental sciencesGE1-350ENEcotoxicology and Environmental Safety, Vol 228, Iss , Pp 113004- (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Chlorella vulgaris Omics approaches Pseudomonas putida Rice Stress amelioration Environmental pollution TD172-193.5 Environmental sciences GE1-350
spellingShingle	Chlorella vulgaris Omics approaches Pseudomonas putida Rice Stress amelioration Environmental pollution TD172-193.5 Environmental sciences GE1-350 Surabhi Awasthi Reshu Chauhan Yuvraj Indoliya Abhishek Singh Chauhan ShashankKumar Mishra Lalit Agrawal Sanjay Dwivedi Shiv Naresh Singh Suchi Srivastava Poonam C. Singh Puneet Singh Chauhan Debasis Chakrabarty Sudhakar Srivastava Rudra Deo Tripathi Microbial consortium mediated growth promotion and Arsenic reduction in Rice: An integrated transcriptome and proteome profiling
description	The adverse effects of arsenic (As) contamination are well known. Rice is a staple food for 50% of the world population but the accumulation of As into rice hampers the food security and safety. Thus the amelioration of As stress and reduction of As levels in rice are needed. In this study, transcriptome (Illumina sequencing) and proteome (2D gel electrophoresis) explored mechanisms of consortium (P. putida+C. vulgaris) mediated growth promotion and As amelioration in rice. The rice seedlings grown hydroponically in the Hewitt nutrient medium and after acclimatization, exposed to 50 µM As alone as well as with microbial consortium to observe the impact at morphological and molecular level. The inoculation of microbial consortium significantly ameliorated the As toxicity, improved growth of root hairs and maintained cellular integrity of the epidermis, exodermis and the stele region during As exposure. Several genes showed differential expression in As and As+P. putida. Down-regulation of As transporters (OsPIP2;2 and OsPIP2;3, OsTIP2;1) and higher expression of WRKY gene (WRKY28) during As+P. putida+C.vulgaris suggested reduction of As uptake in rice. The up-regulation of nutrient elements transporters (OsZIFL9, OsZIFL5, OsZIFL12 and OsZIP2, OsYSL15 and OsCOPT6) in the presence of consortium indicated the improved nutrient status of rice. Higher expression of regulatory elements like auxin/indole 3 acetic acid (AUX/IAA), WRKY and myeloblastosis (MYB) TFs and down-regulation of defense responsive genes such Glutathione-S-transferase, Peroxidase and Glutaredoxinduring As+P. putida+C.vulgaris exposure was also observed. Proteome profiling demonstrated differential abundance of proteins involved in photosynthesis (chlorophyll a/b binding protein, photosystem I Fe-S centre), energy metabolism (ATP synthase subunit beta) transport, signaling (tubulin 1, actin 1), defense (glutathione S-transferase, phenylalanine ammonia lyase) and amino acid metabolism (cysteine synthase, glutamine synthetase), which supported the As ameliorative and growth-promoting potential of microbial consortium during As stress in rice plants. The study gives comprehensive information about gene and protein changes in rice plants in As+consortium exposure.
format	article
author	Surabhi Awasthi Reshu Chauhan Yuvraj Indoliya Abhishek Singh Chauhan ShashankKumar Mishra Lalit Agrawal Sanjay Dwivedi Shiv Naresh Singh Suchi Srivastava Poonam C. Singh Puneet Singh Chauhan Debasis Chakrabarty Sudhakar Srivastava Rudra Deo Tripathi
author_facet	Surabhi Awasthi Reshu Chauhan Yuvraj Indoliya Abhishek Singh Chauhan ShashankKumar Mishra Lalit Agrawal Sanjay Dwivedi Shiv Naresh Singh Suchi Srivastava Poonam C. Singh Puneet Singh Chauhan Debasis Chakrabarty Sudhakar Srivastava Rudra Deo Tripathi
author_sort	Surabhi Awasthi
title	Microbial consortium mediated growth promotion and Arsenic reduction in Rice: An integrated transcriptome and proteome profiling
title_short	Microbial consortium mediated growth promotion and Arsenic reduction in Rice: An integrated transcriptome and proteome profiling
title_full	Microbial consortium mediated growth promotion and Arsenic reduction in Rice: An integrated transcriptome and proteome profiling
title_fullStr	Microbial consortium mediated growth promotion and Arsenic reduction in Rice: An integrated transcriptome and proteome profiling
title_full_unstemmed	Microbial consortium mediated growth promotion and Arsenic reduction in Rice: An integrated transcriptome and proteome profiling
title_sort	microbial consortium mediated growth promotion and arsenic reduction in rice: an integrated transcriptome and proteome profiling
publisher	Elsevier
publishDate	2021
url	https://doaj.org/article/d0e2e154d80c46bca663c3d20464f1c1
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Microbial consortium mediated growth promotion and Arsenic reduction in Rice: An integrated transcriptome and proteome profiling

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