PapRIV, a BV-2 microglial cell activating quorum sensing peptide

Abstract Quorum sensing peptides (QSPs) are bacterial peptides produced by Gram-positive bacteria to communicate with their peers in a cell-density dependent manner. These peptides do not only act as interbacterial communication signals, but can also have effects on the host. Compelling evidence dem...

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Autores principales:	Yorick Janssens, Nathan Debunne, Anton De Spiegeleer, Evelien Wynendaele, Marta Planas, Lidia Feliu, Alessandra Quarta, Christel Claes, Debby Van Dam, Peter Paul De Deyn, Peter Ponsaerts, Matthew Blurton-Jones, Bart De Spiegeleer
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/d0e4551913a54590a13dc55afecc246d
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spelling	oai:doaj.org-article:d0e4551913a54590a13dc55afecc246d2021-12-02T15:45:21ZPapRIV, a BV-2 microglial cell activating quorum sensing peptide10.1038/s41598-021-90030-y2045-2322https://doaj.org/article/d0e4551913a54590a13dc55afecc246d2021-05-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-90030-yhttps://doaj.org/toc/2045-2322Abstract Quorum sensing peptides (QSPs) are bacterial peptides produced by Gram-positive bacteria to communicate with their peers in a cell-density dependent manner. These peptides do not only act as interbacterial communication signals, but can also have effects on the host. Compelling evidence demonstrates the presence of a gut-brain axis and more specifically, the role of the gut microbiota in microglial functioning. The aim of this study is to investigate microglial activating properties of a selected QSP (PapRIV) which is produced by Bacillus cereus species. PapRIV showed in vitro activating properties of BV-2 microglia cells and was able to cross the in vitro Caco-2 cell model and reach the brain. In vivo peptide presence was also demonstrated in mouse plasma. The peptide caused induction of IL-6, TNFα and ROS expression and increased the fraction of ameboid BV-2 microglia cells in an NF-κB dependent manner. Different metabolites were identified in serum, of which the main metabolite still remained active. PapRIV is thus able to cross the gastro-intestinal tract and the blood–brain barrier and shows in vitro activating properties in BV-2 microglia cells, hereby indicating a potential role of this quorum sensing peptide in gut-brain interaction.Yorick JanssensNathan DebunneAnton De SpiegeleerEvelien WynendaeleMarta PlanasLidia FeliuAlessandra QuartaChristel ClaesDebby Van DamPeter Paul De DeynPeter PonsaertsMatthew Blurton-JonesBart De SpiegeleerNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-14 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Yorick Janssens Nathan Debunne Anton De Spiegeleer Evelien Wynendaele Marta Planas Lidia Feliu Alessandra Quarta Christel Claes Debby Van Dam Peter Paul De Deyn Peter Ponsaerts Matthew Blurton-Jones Bart De Spiegeleer PapRIV, a BV-2 microglial cell activating quorum sensing peptide
description	Abstract Quorum sensing peptides (QSPs) are bacterial peptides produced by Gram-positive bacteria to communicate with their peers in a cell-density dependent manner. These peptides do not only act as interbacterial communication signals, but can also have effects on the host. Compelling evidence demonstrates the presence of a gut-brain axis and more specifically, the role of the gut microbiota in microglial functioning. The aim of this study is to investigate microglial activating properties of a selected QSP (PapRIV) which is produced by Bacillus cereus species. PapRIV showed in vitro activating properties of BV-2 microglia cells and was able to cross the in vitro Caco-2 cell model and reach the brain. In vivo peptide presence was also demonstrated in mouse plasma. The peptide caused induction of IL-6, TNFα and ROS expression and increased the fraction of ameboid BV-2 microglia cells in an NF-κB dependent manner. Different metabolites were identified in serum, of which the main metabolite still remained active. PapRIV is thus able to cross the gastro-intestinal tract and the blood–brain barrier and shows in vitro activating properties in BV-2 microglia cells, hereby indicating a potential role of this quorum sensing peptide in gut-brain interaction.
format	article
author	Yorick Janssens Nathan Debunne Anton De Spiegeleer Evelien Wynendaele Marta Planas Lidia Feliu Alessandra Quarta Christel Claes Debby Van Dam Peter Paul De Deyn Peter Ponsaerts Matthew Blurton-Jones Bart De Spiegeleer
author_facet	Yorick Janssens Nathan Debunne Anton De Spiegeleer Evelien Wynendaele Marta Planas Lidia Feliu Alessandra Quarta Christel Claes Debby Van Dam Peter Paul De Deyn Peter Ponsaerts Matthew Blurton-Jones Bart De Spiegeleer
author_sort	Yorick Janssens
title	PapRIV, a BV-2 microglial cell activating quorum sensing peptide
title_short	PapRIV, a BV-2 microglial cell activating quorum sensing peptide
title_full	PapRIV, a BV-2 microglial cell activating quorum sensing peptide
title_fullStr	PapRIV, a BV-2 microglial cell activating quorum sensing peptide
title_full_unstemmed	PapRIV, a BV-2 microglial cell activating quorum sensing peptide
title_sort	papriv, a bv-2 microglial cell activating quorum sensing peptide
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/d0e4551913a54590a13dc55afecc246d
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PapRIV, a BV-2 microglial cell activating quorum sensing peptide

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