DETERMINATION OF AFLATOXIN IN DAIRY FEEDS AND MILK IN SOME SELECTED AREAS OF ETHIOPIA

Aflatoxin B1 (AFB1) in feeds and aflatoxin M1 (AFM1) in milk were studied in Holetta, Bishoftu and Hawassa, Ethiopia using Enzyme-linked Immunosorbent Assay (ELISA). Concentrate feeds were more contaminated (7.67 ± 0.80 μg/kg) than roughage feeds (0.41 ± 0.14 μg/kg); hay was more contaminated (0.72...

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Autores principales:	Rehrahie MESFIN, Getnet ASSEFA, Fassil ASSEFA
Formato:	article
Lenguaje:	EN
Publicado:	Stefan cel Mare University of Suceava 2018
Materias:	Safety; Concentrate; Hay; Straw; Holetta; Bishoftu; Hawassa Food processing and manufacture TP368-456
Acceso en línea:	https://doaj.org/article/d0eb992438014666bec438c78657d4b0
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spelling	oai:doaj.org-article:d0eb992438014666bec438c78657d4b02021-12-02T17:48:49ZDETERMINATION OF AFLATOXIN IN DAIRY FEEDS AND MILK IN SOME SELECTED AREAS OF ETHIOPIA2068-66092559-6381https://doaj.org/article/d0eb992438014666bec438c78657d4b02018-09-01T00:00:00Zhttp://www.fia.usv.ro/fiajournal/index.php/FENS/article/view/597https://doaj.org/toc/2068-6609https://doaj.org/toc/2559-6381Aflatoxin B1 (AFB1) in feeds and aflatoxin M1 (AFM1) in milk were studied in Holetta, Bishoftu and Hawassa, Ethiopia using Enzyme-linked Immunosorbent Assay (ELISA). Concentrate feeds were more contaminated (7.67 ± 0.80 μg/kg) than roughage feeds (0.41 ± 0.14 μg/kg); hay was more contaminated (0.72 ± 0.25 μg/kg) than straw (0.05 ± 0.05 μg/kg) and oilseed cake based concentrate feeds were more contaminated (13.09 ± 1.12 μg/kg) than concentrate feeds without oilseed cake (2.78 ± 0.66 μg/kg). Fifty percent of the feed samples contained 0 µg/kg aflatoxin and 69% and 31% of them fulfilled the permissible limit of the EU (5μg/kg) and the USA (20 μg/kg) for feeds respectively. The average AFB1 of feeds in Bishoftu, Holetta and Hawassa were 9.76µg/kg, 6.33µg/kg and 1.19µg/kg, respectively. The AFB1 of feeds in dairy producers, feed manufacturers and feed retailers were 9.35 ± 1.04µg/kg, 7.50 ± 1.43 µg/kg and 6.91 ± 1.09µg/kg respectively. Twenty nine percent of the milk samples had aflatoxin content of 0 μg/L and; 58% and 42% of them fullfilled the EU (0.05μg/L) and the U.S.A.(0.5 μg/L) permissible limits respectively. Further studies are required by using other techniques such as HPLC, GC and multi-mycotoxin assay using LC-MS-MS. The contribution of too acidic and too alkaline PH on aflaoxin reduction should also be studied. Awareness creation is required to feed processers, feed traders and dairy producers along the feed-milk production and marketing chain. To minimize the incidence of aflatoxin, feed processers, feed traders and dairy producers should employ better feed/grain storage practices.Rehrahie MESFINGetnet ASSEFAFassil ASSEFAStefan cel Mare University of SuceavaarticleSafety; Concentrate; Hay; Straw; Holetta; Bishoftu; HawassaFood processing and manufactureTP368-456ENFood and Environment Safety, Vol 17, Iss 3, Pp 286 -299 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	Safety; Concentrate; Hay; Straw; Holetta; Bishoftu; Hawassa Food processing and manufacture TP368-456
spellingShingle	Safety; Concentrate; Hay; Straw; Holetta; Bishoftu; Hawassa Food processing and manufacture TP368-456 Rehrahie MESFIN Getnet ASSEFA Fassil ASSEFA DETERMINATION OF AFLATOXIN IN DAIRY FEEDS AND MILK IN SOME SELECTED AREAS OF ETHIOPIA
description	Aflatoxin B1 (AFB1) in feeds and aflatoxin M1 (AFM1) in milk were studied in Holetta, Bishoftu and Hawassa, Ethiopia using Enzyme-linked Immunosorbent Assay (ELISA). Concentrate feeds were more contaminated (7.67 ± 0.80 μg/kg) than roughage feeds (0.41 ± 0.14 μg/kg); hay was more contaminated (0.72 ± 0.25 μg/kg) than straw (0.05 ± 0.05 μg/kg) and oilseed cake based concentrate feeds were more contaminated (13.09 ± 1.12 μg/kg) than concentrate feeds without oilseed cake (2.78 ± 0.66 μg/kg). Fifty percent of the feed samples contained 0 µg/kg aflatoxin and 69% and 31% of them fulfilled the permissible limit of the EU (5μg/kg) and the USA (20 μg/kg) for feeds respectively. The average AFB1 of feeds in Bishoftu, Holetta and Hawassa were 9.76µg/kg, 6.33µg/kg and 1.19µg/kg, respectively. The AFB1 of feeds in dairy producers, feed manufacturers and feed retailers were 9.35 ± 1.04µg/kg, 7.50 ± 1.43 µg/kg and 6.91 ± 1.09µg/kg respectively. Twenty nine percent of the milk samples had aflatoxin content of 0 μg/L and; 58% and 42% of them fullfilled the EU (0.05μg/L) and the U.S.A.(0.5 μg/L) permissible limits respectively. Further studies are required by using other techniques such as HPLC, GC and multi-mycotoxin assay using LC-MS-MS. The contribution of too acidic and too alkaline PH on aflaoxin reduction should also be studied. Awareness creation is required to feed processers, feed traders and dairy producers along the feed-milk production and marketing chain. To minimize the incidence of aflatoxin, feed processers, feed traders and dairy producers should employ better feed/grain storage practices.
format	article
author	Rehrahie MESFIN Getnet ASSEFA Fassil ASSEFA
author_facet	Rehrahie MESFIN Getnet ASSEFA Fassil ASSEFA
author_sort	Rehrahie MESFIN
title	DETERMINATION OF AFLATOXIN IN DAIRY FEEDS AND MILK IN SOME SELECTED AREAS OF ETHIOPIA
title_short	DETERMINATION OF AFLATOXIN IN DAIRY FEEDS AND MILK IN SOME SELECTED AREAS OF ETHIOPIA
title_full	DETERMINATION OF AFLATOXIN IN DAIRY FEEDS AND MILK IN SOME SELECTED AREAS OF ETHIOPIA
title_fullStr	DETERMINATION OF AFLATOXIN IN DAIRY FEEDS AND MILK IN SOME SELECTED AREAS OF ETHIOPIA
title_full_unstemmed	DETERMINATION OF AFLATOXIN IN DAIRY FEEDS AND MILK IN SOME SELECTED AREAS OF ETHIOPIA
title_sort	determination of aflatoxin in dairy feeds and milk in some selected areas of ethiopia
publisher	Stefan cel Mare University of Suceava
publishDate	2018
url	https://doaj.org/article/d0eb992438014666bec438c78657d4b0
work_keys_str_mv	AT rehrahiemesfin determinationofaflatoxinindairyfeedsandmilkinsomeselectedareasofethiopia AT getnetassefa determinationofaflatoxinindairyfeedsandmilkinsomeselectedareasofethiopia AT fassilassefa determinationofaflatoxinindairyfeedsandmilkinsomeselectedareasofethiopia
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DETERMINATION OF AFLATOXIN IN DAIRY FEEDS AND MILK IN SOME SELECTED AREAS OF ETHIOPIA

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