Examining multiple cellular pathways at once using multiplex hextuple luciferase assaying

Multiplexed detection of luciferase-based sensors in the same sample is challenging and limited by the substrates’ emission spectra. Here the authors establish a system based on three different luciferases and sequential detection to achieve measurements of up to six parameters within the same exper...

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Autores principales:	Alejandro Sarrion-Perdigones, Lyra Chang, Yezabel Gonzalez, Tatiana Gallego-Flores, Damian W. Young, Koen J. T. Venken
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2019
Materias:	Science Q
Acceso en línea:	https://doaj.org/article/d0eba81dab4c416f9ae7a95bfa426800
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spelling	oai:doaj.org-article:d0eba81dab4c416f9ae7a95bfa4268002021-12-02T15:35:10ZExamining multiple cellular pathways at once using multiplex hextuple luciferase assaying10.1038/s41467-019-13651-y2041-1723https://doaj.org/article/d0eba81dab4c416f9ae7a95bfa4268002019-12-01T00:00:00Zhttps://doi.org/10.1038/s41467-019-13651-yhttps://doaj.org/toc/2041-1723Multiplexed detection of luciferase-based sensors in the same sample is challenging and limited by the substrates’ emission spectra. Here the authors establish a system based on three different luciferases and sequential detection to achieve measurements of up to six parameters within the same experiment.Alejandro Sarrion-PerdigonesLyra ChangYezabel GonzalezTatiana Gallego-FloresDamian W. YoungKoen J. T. VenkenNature PortfolioarticleScienceQENNature Communications, Vol 10, Iss 1, Pp 1-16 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	Science Q
spellingShingle	Science Q Alejandro Sarrion-Perdigones Lyra Chang Yezabel Gonzalez Tatiana Gallego-Flores Damian W. Young Koen J. T. Venken Examining multiple cellular pathways at once using multiplex hextuple luciferase assaying
description	Multiplexed detection of luciferase-based sensors in the same sample is challenging and limited by the substrates’ emission spectra. Here the authors establish a system based on three different luciferases and sequential detection to achieve measurements of up to six parameters within the same experiment.
format	article
author	Alejandro Sarrion-Perdigones Lyra Chang Yezabel Gonzalez Tatiana Gallego-Flores Damian W. Young Koen J. T. Venken
author_facet	Alejandro Sarrion-Perdigones Lyra Chang Yezabel Gonzalez Tatiana Gallego-Flores Damian W. Young Koen J. T. Venken
author_sort	Alejandro Sarrion-Perdigones
title	Examining multiple cellular pathways at once using multiplex hextuple luciferase assaying
title_short	Examining multiple cellular pathways at once using multiplex hextuple luciferase assaying
title_full	Examining multiple cellular pathways at once using multiplex hextuple luciferase assaying
title_fullStr	Examining multiple cellular pathways at once using multiplex hextuple luciferase assaying
title_full_unstemmed	Examining multiple cellular pathways at once using multiplex hextuple luciferase assaying
title_sort	examining multiple cellular pathways at once using multiplex hextuple luciferase assaying
publisher	Nature Portfolio
publishDate	2019
url	https://doaj.org/article/d0eba81dab4c416f9ae7a95bfa426800
work_keys_str_mv	AT alejandrosarrionperdigones examiningmultiplecellularpathwaysatonceusingmultiplexhextupleluciferaseassaying AT lyrachang examiningmultiplecellularpathwaysatonceusingmultiplexhextupleluciferaseassaying AT yezabelgonzalez examiningmultiplecellularpathwaysatonceusingmultiplexhextupleluciferaseassaying AT tatianagallegoflores examiningmultiplecellularpathwaysatonceusingmultiplexhextupleluciferaseassaying AT damianwyoung examiningmultiplecellularpathwaysatonceusingmultiplexhextupleluciferaseassaying AT koenjtvenken examiningmultiplecellularpathwaysatonceusingmultiplexhextupleluciferaseassaying
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Examining multiple cellular pathways at once using multiplex hextuple luciferase assaying

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