Examining multiple cellular pathways at once using multiplex hextuple luciferase assaying
Multiplexed detection of luciferase-based sensors in the same sample is challenging and limited by the substrates’ emission spectra. Here the authors establish a system based on three different luciferases and sequential detection to achieve measurements of up to six parameters within the same exper...
Guardado en:
Autores principales: | Alejandro Sarrion-Perdigones, Lyra Chang, Yezabel Gonzalez, Tatiana Gallego-Flores, Damian W. Young, Koen J. T. Venken |
---|---|
Formato: | article |
Lenguaje: | EN |
Publicado: |
Nature Portfolio
2019
|
Materias: | |
Acceso en línea: | https://doaj.org/article/d0eba81dab4c416f9ae7a95bfa426800 |
Etiquetas: |
Agregar Etiqueta
Sin Etiquetas, Sea el primero en etiquetar este registro!
|
Ejemplares similares
-
A novel thermostable beetle luciferase based cytotoxicity assay
por: Sunju Choi, et al.
Publicado: (2021) -
A homogeneous split-luciferase assay for rapid and sensitive detection of anti-SARS CoV-2 antibodies
por: Zhong Yao, et al.
Publicado: (2021) -
Improving the Stability of Protein–Protein Interaction Assay FlimPIA Using a Thermostabilized Firefly Luciferase
por: Yuki Ohmuro-Matsuyama, et al.
Publicado: (2021) -
Examining the Possibility of Detecting Brucella Canis from Tissue Samples Using Bruce-Ladder Multiplex PCR Assay
por: Stević Nataša, et al.
Publicado: (2017) -
Engineering the protein dynamics of an ancestral luciferase
por: Andrea Schenkmayerova, et al.
Publicado: (2021)