Examining multiple cellular pathways at once using multiplex hextuple luciferase assaying
Multiplexed detection of luciferase-based sensors in the same sample is challenging and limited by the substrates’ emission spectra. Here the authors establish a system based on three different luciferases and sequential detection to achieve measurements of up to six parameters within the same exper...
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Main Authors: | , , , , , |
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Format: | article |
Language: | EN |
Published: |
Nature Portfolio
2019
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Subjects: | |
Online Access: | https://doaj.org/article/d0eba81dab4c416f9ae7a95bfa426800 |
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