Analysis of gene expression and proteomic profiles of clonal genotypes from Theobroma cacao subjected to soil flooding.

Soil flooding causes changes in gene transcription, synthesis and degradation of proteins and cell metabolism. The main objective of this study was to understand the biological events of Theobroma cacao during soil flooding-induced stress, using the analyses of gene expression and activity of key en...

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Autores principales:	Fabiana Z Bertolde, Alex-Alan F Almeida, Carlos P Pirovani
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2014
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/d0ec851d4cca482ab7969bc927da34ae
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spelling	oai:doaj.org-article:d0ec851d4cca482ab7969bc927da34ae2021-11-25T05:57:43ZAnalysis of gene expression and proteomic profiles of clonal genotypes from Theobroma cacao subjected to soil flooding.1932-620310.1371/journal.pone.0108705https://doaj.org/article/d0ec851d4cca482ab7969bc927da34ae2014-01-01T00:00:00Zhttps://doi.org/10.1371/journal.pone.0108705https://doaj.org/toc/1932-6203Soil flooding causes changes in gene transcription, synthesis and degradation of proteins and cell metabolism. The main objective of this study was to understand the biological events of Theobroma cacao during soil flooding-induced stress, using the analyses of gene expression and activity of key enzymes involved in fermentation, as well as the identification of differentially expressed proteins by mass spectrometry in two contrasting genotypes for flooding tolerance (tolerant - TSA-792 and susceptible - TSH-774). Soil anoxia caused by flooding has led to changes in the expression pattern of genes associated with the biosynthesis of alcohol dehydrogenase (ADH), pyruvate decarboxylase (PDC) and lactate dehydrogenase (LDH) in leaves and roots of the two evaluated genotypes. Significant differences were observed between the enzyme activities of the two genotypes. Leaves and roots of the TSA-792 genotype showed higher ADH activity as compared to the TSH-774 genotype, whereas the activities of PDC and LDH have varied over the 96 h of soil flooding, being higher for TSA-792 genotype, at the initial stage, and TSH-774 genotype, at the final stage. Some of the identified proteins are those typical of the anaerobic metabolism-involved in glycolysis and alcoholic fermentation-and different proteins associated with photosynthesis, protein metabolism and oxidative stress. The ability to maintain glycolysis and induce fermentation was observed to play an important role in anoxia tolerance in cacao and may also serve to distinguish tolerant and susceptible genotypes in relation to this stressor.Fabiana Z BertoldeAlex-Alan F AlmeidaCarlos P PirovaniPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 9, Iss 10, p e108705 (2014)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Fabiana Z Bertolde Alex-Alan F Almeida Carlos P Pirovani Analysis of gene expression and proteomic profiles of clonal genotypes from Theobroma cacao subjected to soil flooding.
description	Soil flooding causes changes in gene transcription, synthesis and degradation of proteins and cell metabolism. The main objective of this study was to understand the biological events of Theobroma cacao during soil flooding-induced stress, using the analyses of gene expression and activity of key enzymes involved in fermentation, as well as the identification of differentially expressed proteins by mass spectrometry in two contrasting genotypes for flooding tolerance (tolerant - TSA-792 and susceptible - TSH-774). Soil anoxia caused by flooding has led to changes in the expression pattern of genes associated with the biosynthesis of alcohol dehydrogenase (ADH), pyruvate decarboxylase (PDC) and lactate dehydrogenase (LDH) in leaves and roots of the two evaluated genotypes. Significant differences were observed between the enzyme activities of the two genotypes. Leaves and roots of the TSA-792 genotype showed higher ADH activity as compared to the TSH-774 genotype, whereas the activities of PDC and LDH have varied over the 96 h of soil flooding, being higher for TSA-792 genotype, at the initial stage, and TSH-774 genotype, at the final stage. Some of the identified proteins are those typical of the anaerobic metabolism-involved in glycolysis and alcoholic fermentation-and different proteins associated with photosynthesis, protein metabolism and oxidative stress. The ability to maintain glycolysis and induce fermentation was observed to play an important role in anoxia tolerance in cacao and may also serve to distinguish tolerant and susceptible genotypes in relation to this stressor.
format	article
author	Fabiana Z Bertolde Alex-Alan F Almeida Carlos P Pirovani
author_facet	Fabiana Z Bertolde Alex-Alan F Almeida Carlos P Pirovani
author_sort	Fabiana Z Bertolde
title	Analysis of gene expression and proteomic profiles of clonal genotypes from Theobroma cacao subjected to soil flooding.
title_short	Analysis of gene expression and proteomic profiles of clonal genotypes from Theobroma cacao subjected to soil flooding.
title_full	Analysis of gene expression and proteomic profiles of clonal genotypes from Theobroma cacao subjected to soil flooding.
title_fullStr	Analysis of gene expression and proteomic profiles of clonal genotypes from Theobroma cacao subjected to soil flooding.
title_full_unstemmed	Analysis of gene expression and proteomic profiles of clonal genotypes from Theobroma cacao subjected to soil flooding.
title_sort	analysis of gene expression and proteomic profiles of clonal genotypes from theobroma cacao subjected to soil flooding.
publisher	Public Library of Science (PLoS)
publishDate	2014
url	https://doaj.org/article/d0ec851d4cca482ab7969bc927da34ae
work_keys_str_mv	AT fabianazbertolde analysisofgeneexpressionandproteomicprofilesofclonalgenotypesfromtheobromacacaosubjectedtosoilflooding AT alexalanfalmeida analysisofgeneexpressionandproteomicprofilesofclonalgenotypesfromtheobromacacaosubjectedtosoilflooding AT carlosppirovani analysisofgeneexpressionandproteomicprofilesofclonalgenotypesfromtheobromacacaosubjectedtosoilflooding
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Analysis of gene expression and proteomic profiles of clonal genotypes from Theobroma cacao subjected to soil flooding.

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