INsPECT, an open-source and versatile software for automated quantification of (Leishmania) intracellular parasites.

Intracellular protozoan parasites are causative agents of infectious diseases that constitute major health problems for developing countries. Leishmania sp., Trypanosoma cruzi or Toxoplasma gondii are all obligate intracellular protozoan parasites that reside and multiply within the host cells of ma...

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Autores principales:	Ehsan Yazdanparast, Antonio Dos Anjos, Deborah Garcia, Corinne Loeuillet, Hamid Reza Shahbazkia, Baptiste Vergnes
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2014
Materias:	Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270
Acceso en línea:	https://doaj.org/article/d0ee963abc8541eb985b73525deaea87
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spelling	oai:doaj.org-article:d0ee963abc8541eb985b73525deaea872021-11-18T09:16:04ZINsPECT, an open-source and versatile software for automated quantification of (Leishmania) intracellular parasites.1935-27271935-273510.1371/journal.pntd.0002850https://doaj.org/article/d0ee963abc8541eb985b73525deaea872014-05-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24831235/?tool=EBIhttps://doaj.org/toc/1935-2727https://doaj.org/toc/1935-2735Intracellular protozoan parasites are causative agents of infectious diseases that constitute major health problems for developing countries. Leishmania sp., Trypanosoma cruzi or Toxoplasma gondii are all obligate intracellular protozoan parasites that reside and multiply within the host cells of mammals, including humans. Following up intracellular parasite proliferation is therefore an essential and a quotidian task for many laboratories working on primary screening of new natural and synthetic drugs, analyzing drug susceptibility or comparing virulence properties of natural and genetically modified strains. Nevertheless, laborious manual microscopic counting of intracellular parasites is still the most commonly used approach. Here, we present INsPECT (Intracellular ParasitE CounTer), an open-source and platform independent software dedicated to automate infection level measurement based on fluorescent DNA staining. It offers the possibility to choose between different types of analyses (fluorescent DNA acquisitions only or in combination with phase contrast image set to further separate intra- from extracellular parasites), and software running modes (automatic or custom). A proof-of-concept study with intracellular Leishmania infantum parasites stained with DAPI (4',6-diamidino-2-phenylindole) confirms a good correspondence between digital results and the "gold standard" microscopic counting method with Giemsa. Interestingly, this software is versatile enough to accurately detect intracellular T. gondii parasites on images acquired with High Content Screening (HCS) systems. In conclusion, INsPECT software is proposed as a new fast and simple alternative to the classical intracellular Leishmania quantification methods and can be adapted for mid to large-scale drug screening against different intracellular parasites.Ehsan YazdanparastAntonio Dos AnjosDeborah GarciaCorinne LoeuilletHamid Reza ShahbazkiaBaptiste VergnesPublic Library of Science (PLoS)articleArctic medicine. Tropical medicineRC955-962Public aspects of medicineRA1-1270ENPLoS Neglected Tropical Diseases, Vol 8, Iss 5, p e2850 (2014)
institution	DOAJ
collection	DOAJ
language	EN
topic	Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270
spellingShingle	Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 Ehsan Yazdanparast Antonio Dos Anjos Deborah Garcia Corinne Loeuillet Hamid Reza Shahbazkia Baptiste Vergnes INsPECT, an open-source and versatile software for automated quantification of (Leishmania) intracellular parasites.
description	Intracellular protozoan parasites are causative agents of infectious diseases that constitute major health problems for developing countries. Leishmania sp., Trypanosoma cruzi or Toxoplasma gondii are all obligate intracellular protozoan parasites that reside and multiply within the host cells of mammals, including humans. Following up intracellular parasite proliferation is therefore an essential and a quotidian task for many laboratories working on primary screening of new natural and synthetic drugs, analyzing drug susceptibility or comparing virulence properties of natural and genetically modified strains. Nevertheless, laborious manual microscopic counting of intracellular parasites is still the most commonly used approach. Here, we present INsPECT (Intracellular ParasitE CounTer), an open-source and platform independent software dedicated to automate infection level measurement based on fluorescent DNA staining. It offers the possibility to choose between different types of analyses (fluorescent DNA acquisitions only or in combination with phase contrast image set to further separate intra- from extracellular parasites), and software running modes (automatic or custom). A proof-of-concept study with intracellular Leishmania infantum parasites stained with DAPI (4',6-diamidino-2-phenylindole) confirms a good correspondence between digital results and the "gold standard" microscopic counting method with Giemsa. Interestingly, this software is versatile enough to accurately detect intracellular T. gondii parasites on images acquired with High Content Screening (HCS) systems. In conclusion, INsPECT software is proposed as a new fast and simple alternative to the classical intracellular Leishmania quantification methods and can be adapted for mid to large-scale drug screening against different intracellular parasites.
format	article
author	Ehsan Yazdanparast Antonio Dos Anjos Deborah Garcia Corinne Loeuillet Hamid Reza Shahbazkia Baptiste Vergnes
author_facet	Ehsan Yazdanparast Antonio Dos Anjos Deborah Garcia Corinne Loeuillet Hamid Reza Shahbazkia Baptiste Vergnes
author_sort	Ehsan Yazdanparast
title	INsPECT, an open-source and versatile software for automated quantification of (Leishmania) intracellular parasites.
title_short	INsPECT, an open-source and versatile software for automated quantification of (Leishmania) intracellular parasites.
title_full	INsPECT, an open-source and versatile software for automated quantification of (Leishmania) intracellular parasites.
title_fullStr	INsPECT, an open-source and versatile software for automated quantification of (Leishmania) intracellular parasites.
title_full_unstemmed	INsPECT, an open-source and versatile software for automated quantification of (Leishmania) intracellular parasites.
title_sort	inspect, an open-source and versatile software for automated quantification of (leishmania) intracellular parasites.
publisher	Public Library of Science (PLoS)
publishDate	2014
url	https://doaj.org/article/d0ee963abc8541eb985b73525deaea87
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INsPECT, an open-source and versatile software for automated quantification of (Leishmania) intracellular parasites.

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