Human cell based directed evolution of adenine base editors with improved efficiency

Application of adenine base editors (ABE) has been precluded by low activity. Here the authors show the generation of a human cell based ABE directional screening system and identification of ABE variant (NG-ABEmax-KR) exhibiting a significant increase in activity for human and mouse genome manipula...

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Auteurs principaux:	Junhao Fu, Qing Li, Xiaoyu Liu, Tianxiang Tu, Xiujuan Lv, Xidi Yin, Jineng Lv, Zongming Song, Jia Qu, Jinwei Zhang, Jinsong Li, Feng Gu
Format:	article
Langue:	EN
Publié:	Nature Portfolio 2021
Sujets:	Science Q
Accès en ligne:	https://doaj.org/article/d0f00b9c200741e59b1418eaed8eed7c
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spelling	oai:doaj.org-article:d0f00b9c200741e59b1418eaed8eed7c2021-12-02T19:16:24ZHuman cell based directed evolution of adenine base editors with improved efficiency10.1038/s41467-021-26211-02041-1723https://doaj.org/article/d0f00b9c200741e59b1418eaed8eed7c2021-10-01T00:00:00Zhttps://doi.org/10.1038/s41467-021-26211-0https://doaj.org/toc/2041-1723Application of adenine base editors (ABE) has been precluded by low activity. Here the authors show the generation of a human cell based ABE directional screening system and identification of ABE variant (NG-ABEmax-KR) exhibiting a significant increase in activity for human and mouse genome manipulation.Junhao FuQing LiXiaoyu LiuTianxiang TuXiujuan LvXidi YinJineng LvZongming SongJia QuJinwei ZhangJinsong LiFeng GuNature PortfolioarticleScienceQENNature Communications, Vol 12, Iss 1, Pp 1-11 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Science Q
spellingShingle	Science Q Junhao Fu Qing Li Xiaoyu Liu Tianxiang Tu Xiujuan Lv Xidi Yin Jineng Lv Zongming Song Jia Qu Jinwei Zhang Jinsong Li Feng Gu Human cell based directed evolution of adenine base editors with improved efficiency
description	Application of adenine base editors (ABE) has been precluded by low activity. Here the authors show the generation of a human cell based ABE directional screening system and identification of ABE variant (NG-ABEmax-KR) exhibiting a significant increase in activity for human and mouse genome manipulation.
format	article
author	Junhao Fu Qing Li Xiaoyu Liu Tianxiang Tu Xiujuan Lv Xidi Yin Jineng Lv Zongming Song Jia Qu Jinwei Zhang Jinsong Li Feng Gu
author_facet	Junhao Fu Qing Li Xiaoyu Liu Tianxiang Tu Xiujuan Lv Xidi Yin Jineng Lv Zongming Song Jia Qu Jinwei Zhang Jinsong Li Feng Gu
author_sort	Junhao Fu
title	Human cell based directed evolution of adenine base editors with improved efficiency
title_short	Human cell based directed evolution of adenine base editors with improved efficiency
title_full	Human cell based directed evolution of adenine base editors with improved efficiency
title_fullStr	Human cell based directed evolution of adenine base editors with improved efficiency
title_full_unstemmed	Human cell based directed evolution of adenine base editors with improved efficiency
title_sort	human cell based directed evolution of adenine base editors with improved efficiency
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/d0f00b9c200741e59b1418eaed8eed7c
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_version_	1718376939090083840

Human cell based directed evolution of adenine base editors with improved efficiency

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