Human cell based directed evolution of adenine base editors with improved efficiency

Human cell based directed evolution of adenine base editors with improved efficiency

Application of adenine base editors (ABE) has been precluded by low activity. Here the authors show the generation of a human cell based ABE directional screening system and identification of ABE variant (NG-ABEmax-KR) exhibiting a significant increase in activity for human and mouse genome manipula...

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Autores principales: Junhao Fu, Qing Li, Xiaoyu Liu, Tianxiang Tu, Xiujuan Lv, Xidi Yin, Jineng Lv, Zongming Song, Jia Qu, Jinwei Zhang, Jinsong Li, Feng Gu
Formato: article
Lenguaje:EN
Publicado: Nature Portfolio 2021
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Acceso en línea:https://doaj.org/article/d0f00b9c200741e59b1418eaed8eed7c
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spelling oai:doaj.org-article:d0f00b9c200741e59b1418eaed8eed7c2021-12-02T19:16:24ZHuman cell based directed evolution of adenine base editors with improved efficiency10.1038/s41467-021-26211-02041-1723https://doaj.org/article/d0f00b9c200741e59b1418eaed8eed7c2021-10-01T00:00:00Zhttps://doi.org/10.1038/s41467-021-26211-0https://doaj.org/toc/2041-1723Application of adenine base editors (ABE) has been precluded by low activity. Here the authors show the generation of a human cell based ABE directional screening system and identification of ABE variant (NG-ABEmax-KR) exhibiting a significant increase in activity for human and mouse genome manipulation.Junhao FuQing LiXiaoyu LiuTianxiang TuXiujuan LvXidi YinJineng LvZongming SongJia QuJinwei ZhangJinsong LiFeng GuNature PortfolioarticleScienceQENNature Communications, Vol 12, Iss 1, Pp 1-11 (2021)
institution DOAJ
collection DOAJ
language EN
topic Science
Q
spellingShingle Science
Q
Junhao Fu
Qing Li
Xiaoyu Liu
Tianxiang Tu
Xiujuan Lv
Xidi Yin
Jineng Lv
Zongming Song
Jia Qu
Jinwei Zhang
Jinsong Li
Feng Gu
Human cell based directed evolution of adenine base editors with improved efficiency
description Application of adenine base editors (ABE) has been precluded by low activity. Here the authors show the generation of a human cell based ABE directional screening system and identification of ABE variant (NG-ABEmax-KR) exhibiting a significant increase in activity for human and mouse genome manipulation.
format article
author Junhao Fu
Qing Li
Xiaoyu Liu
Tianxiang Tu
Xiujuan Lv
Xidi Yin
Jineng Lv
Zongming Song
Jia Qu
Jinwei Zhang
Jinsong Li
Feng Gu
author_facet Junhao Fu
Qing Li
Xiaoyu Liu
Tianxiang Tu
Xiujuan Lv
Xidi Yin
Jineng Lv
Zongming Song
Jia Qu
Jinwei Zhang
Jinsong Li
Feng Gu
author_sort Junhao Fu
title Human cell based directed evolution of adenine base editors with improved efficiency
title_short Human cell based directed evolution of adenine base editors with improved efficiency
title_full Human cell based directed evolution of adenine base editors with improved efficiency
title_fullStr Human cell based directed evolution of adenine base editors with improved efficiency
title_full_unstemmed Human cell based directed evolution of adenine base editors with improved efficiency
title_sort human cell based directed evolution of adenine base editors with improved efficiency
publisher Nature Portfolio
publishDate 2021
url https://doaj.org/article/d0f00b9c200741e59b1418eaed8eed7c
work_keys_str_mv AT junhaofu humancellbaseddirectedevolutionofadeninebaseeditorswithimprovedefficiency
AT qingli humancellbaseddirectedevolutionofadeninebaseeditorswithimprovedefficiency
AT xiaoyuliu humancellbaseddirectedevolutionofadeninebaseeditorswithimprovedefficiency
AT tianxiangtu humancellbaseddirectedevolutionofadeninebaseeditorswithimprovedefficiency
AT xiujuanlv humancellbaseddirectedevolutionofadeninebaseeditorswithimprovedefficiency
AT xidiyin humancellbaseddirectedevolutionofadeninebaseeditorswithimprovedefficiency
AT jinenglv humancellbaseddirectedevolutionofadeninebaseeditorswithimprovedefficiency
AT zongmingsong humancellbaseddirectedevolutionofadeninebaseeditorswithimprovedefficiency
AT jiaqu humancellbaseddirectedevolutionofadeninebaseeditorswithimprovedefficiency
AT jinweizhang humancellbaseddirectedevolutionofadeninebaseeditorswithimprovedefficiency
AT jinsongli humancellbaseddirectedevolutionofadeninebaseeditorswithimprovedefficiency
AT fenggu humancellbaseddirectedevolutionofadeninebaseeditorswithimprovedefficiency
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