Detection of glioma infiltration at the tumor margin using quantitative stimulated Raman scattering histology

Abstract In the management of diffuse gliomas, the identification and removal of tumor at the infiltrative margin remains a central challenge. Prior work has demonstrated that fluorescence labeling tools and radiographic imaging are useful surgical adjuvants with macroscopic resolution. However, the...

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Autores principales:	Melike Pekmezci, Ramin A. Morshed, Pranathi Chunduru, Balaji Pandian, Jacob Young, Javier E. Villanueva-Meyer, Tarik Tihan, Emily A. Sloan, Manish K. Aghi, Annette M. Molinaro, Mitchel S. Berger, Shawn L. Hervey-Jumper
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/d0f2d439f7c344898da0426b8b2216a6
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spelling	oai:doaj.org-article:d0f2d439f7c344898da0426b8b2216a62021-12-02T17:52:31ZDetection of glioma infiltration at the tumor margin using quantitative stimulated Raman scattering histology10.1038/s41598-021-91648-82045-2322https://doaj.org/article/d0f2d439f7c344898da0426b8b2216a62021-06-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-91648-8https://doaj.org/toc/2045-2322Abstract In the management of diffuse gliomas, the identification and removal of tumor at the infiltrative margin remains a central challenge. Prior work has demonstrated that fluorescence labeling tools and radiographic imaging are useful surgical adjuvants with macroscopic resolution. However, they lose sensitivity at the tumor margin and have limited clinical utility for lower grade histologies. Fiber-laser based stimulated Raman histology (SRH) is an optical imaging technique that provides microscopic tissue characterization of unprocessed tissues. It remains unknown whether SRH of tissues taken from the infiltrative glioma margin will identify microscopic residual disease. Here we acquired glioma margin specimens for SRH, histology, and tumor specific tissue characterization. Generalized linear mixed models were used to evaluate agreement. We find that SRH identified residual tumor in 82 of 167 margin specimens (49%), compared to IHC confirming residual tumor in 72 of 128 samples (56%), and H&E confirming residual tumor in 82 of 169 samples (49%). Intraobserver agreements between all 3 modalities were confirmed. These data demonstrate that SRH detects residual microscopic tumor at the infiltrative glioma margin and may be a promising tool to enhance extent of resection.Melike PekmezciRamin A. MorshedPranathi ChunduruBalaji PandianJacob YoungJavier E. Villanueva-MeyerTarik TihanEmily A. SloanManish K. AghiAnnette M. MolinaroMitchel S. BergerShawn L. Hervey-JumperNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-11 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Melike Pekmezci Ramin A. Morshed Pranathi Chunduru Balaji Pandian Jacob Young Javier E. Villanueva-Meyer Tarik Tihan Emily A. Sloan Manish K. Aghi Annette M. Molinaro Mitchel S. Berger Shawn L. Hervey-Jumper Detection of glioma infiltration at the tumor margin using quantitative stimulated Raman scattering histology
description	Abstract In the management of diffuse gliomas, the identification and removal of tumor at the infiltrative margin remains a central challenge. Prior work has demonstrated that fluorescence labeling tools and radiographic imaging are useful surgical adjuvants with macroscopic resolution. However, they lose sensitivity at the tumor margin and have limited clinical utility for lower grade histologies. Fiber-laser based stimulated Raman histology (SRH) is an optical imaging technique that provides microscopic tissue characterization of unprocessed tissues. It remains unknown whether SRH of tissues taken from the infiltrative glioma margin will identify microscopic residual disease. Here we acquired glioma margin specimens for SRH, histology, and tumor specific tissue characterization. Generalized linear mixed models were used to evaluate agreement. We find that SRH identified residual tumor in 82 of 167 margin specimens (49%), compared to IHC confirming residual tumor in 72 of 128 samples (56%), and H&E confirming residual tumor in 82 of 169 samples (49%). Intraobserver agreements between all 3 modalities were confirmed. These data demonstrate that SRH detects residual microscopic tumor at the infiltrative glioma margin and may be a promising tool to enhance extent of resection.
format	article
author	Melike Pekmezci Ramin A. Morshed Pranathi Chunduru Balaji Pandian Jacob Young Javier E. Villanueva-Meyer Tarik Tihan Emily A. Sloan Manish K. Aghi Annette M. Molinaro Mitchel S. Berger Shawn L. Hervey-Jumper
author_facet	Melike Pekmezci Ramin A. Morshed Pranathi Chunduru Balaji Pandian Jacob Young Javier E. Villanueva-Meyer Tarik Tihan Emily A. Sloan Manish K. Aghi Annette M. Molinaro Mitchel S. Berger Shawn L. Hervey-Jumper
author_sort	Melike Pekmezci
title	Detection of glioma infiltration at the tumor margin using quantitative stimulated Raman scattering histology
title_short	Detection of glioma infiltration at the tumor margin using quantitative stimulated Raman scattering histology
title_full	Detection of glioma infiltration at the tumor margin using quantitative stimulated Raman scattering histology
title_fullStr	Detection of glioma infiltration at the tumor margin using quantitative stimulated Raman scattering histology
title_full_unstemmed	Detection of glioma infiltration at the tumor margin using quantitative stimulated Raman scattering histology
title_sort	detection of glioma infiltration at the tumor margin using quantitative stimulated raman scattering histology
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/d0f2d439f7c344898da0426b8b2216a6
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Detection of glioma infiltration at the tumor margin using quantitative stimulated Raman scattering histology

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