Development of hematopoietic syndrome mice model for localized radiation exposure

Abstract Current models to study the hematopoietic syndrome largely rely on the uniform whole-body exposures. However, in the radio-nuclear accidents or terrorist events, exposure can be non-uniform. The data available on the non-uniform exposures is limited. Thus, we have developed a mice model for...

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Autores principales:	M. H. Yashavarddhan, Ajay Kumar Sharma, Pankaj Chaudhary, Sania Bajaj, Sukhvir Singh, Sandeep Kumar Shukla
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/d0f3564a89ec42b6a0fd3abb5eb26578
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spelling	oai:doaj.org-article:d0f3564a89ec42b6a0fd3abb5eb265782021-12-02T15:12:52ZDevelopment of hematopoietic syndrome mice model for localized radiation exposure10.1038/s41598-020-80075-w2045-2322https://doaj.org/article/d0f3564a89ec42b6a0fd3abb5eb265782021-01-01T00:00:00Zhttps://doi.org/10.1038/s41598-020-80075-whttps://doaj.org/toc/2045-2322Abstract Current models to study the hematopoietic syndrome largely rely on the uniform whole-body exposures. However, in the radio-nuclear accidents or terrorist events, exposure can be non-uniform. The data available on the non-uniform exposures is limited. Thus, we have developed a mice model for studying the hematopoietic syndrome in the non-uniform or partial body exposure scenarios using the localized cobalt60 gamma radiation exposure. Femur region of Strain ‘A’ male mice was exposed to doses ranging from 7 to 20 Gy. The 30 day survival assay showed 19 Gy as LD100 and 17 Gy as LD50. We measured an array of cytokines and important stem cell markers such as IFN-γ, IL-3, IL-6, GM-CSF, TNF-α, G-CSF, IL-1α, IL-1β, CD 34 and Sca 1. We found significant changes in IL-6, GM-CSF, TNF-α, G-CSF, and IL-1β levels compared to untreated groups and amplified levels of CD 34 and Sca 1 positive population in the irradiated mice compared to the untreated controls. Overall, we have developed a mouse model of the hematopoietic acute radiation syndrome that might be useful for understanding of the non-uniform body exposure scenarios. This may also be helpful in the screening of drugs intended for individuals suffering from radiation induced hematopoietic syndrome.M. H. YashavarddhanAjay Kumar SharmaPankaj ChaudharySania BajajSukhvir SinghSandeep Kumar ShuklaNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q M. H. Yashavarddhan Ajay Kumar Sharma Pankaj Chaudhary Sania Bajaj Sukhvir Singh Sandeep Kumar Shukla Development of hematopoietic syndrome mice model for localized radiation exposure
description	Abstract Current models to study the hematopoietic syndrome largely rely on the uniform whole-body exposures. However, in the radio-nuclear accidents or terrorist events, exposure can be non-uniform. The data available on the non-uniform exposures is limited. Thus, we have developed a mice model for studying the hematopoietic syndrome in the non-uniform or partial body exposure scenarios using the localized cobalt60 gamma radiation exposure. Femur region of Strain ‘A’ male mice was exposed to doses ranging from 7 to 20 Gy. The 30 day survival assay showed 19 Gy as LD100 and 17 Gy as LD50. We measured an array of cytokines and important stem cell markers such as IFN-γ, IL-3, IL-6, GM-CSF, TNF-α, G-CSF, IL-1α, IL-1β, CD 34 and Sca 1. We found significant changes in IL-6, GM-CSF, TNF-α, G-CSF, and IL-1β levels compared to untreated groups and amplified levels of CD 34 and Sca 1 positive population in the irradiated mice compared to the untreated controls. Overall, we have developed a mouse model of the hematopoietic acute radiation syndrome that might be useful for understanding of the non-uniform body exposure scenarios. This may also be helpful in the screening of drugs intended for individuals suffering from radiation induced hematopoietic syndrome.
format	article
author	M. H. Yashavarddhan Ajay Kumar Sharma Pankaj Chaudhary Sania Bajaj Sukhvir Singh Sandeep Kumar Shukla
author_facet	M. H. Yashavarddhan Ajay Kumar Sharma Pankaj Chaudhary Sania Bajaj Sukhvir Singh Sandeep Kumar Shukla
author_sort	M. H. Yashavarddhan
title	Development of hematopoietic syndrome mice model for localized radiation exposure
title_short	Development of hematopoietic syndrome mice model for localized radiation exposure
title_full	Development of hematopoietic syndrome mice model for localized radiation exposure
title_fullStr	Development of hematopoietic syndrome mice model for localized radiation exposure
title_full_unstemmed	Development of hematopoietic syndrome mice model for localized radiation exposure
title_sort	development of hematopoietic syndrome mice model for localized radiation exposure
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/d0f3564a89ec42b6a0fd3abb5eb26578
work_keys_str_mv	AT mhyashavarddhan developmentofhematopoieticsyndromemicemodelforlocalizedradiationexposure AT ajaykumarsharma developmentofhematopoieticsyndromemicemodelforlocalizedradiationexposure AT pankajchaudhary developmentofhematopoieticsyndromemicemodelforlocalizedradiationexposure AT saniabajaj developmentofhematopoieticsyndromemicemodelforlocalizedradiationexposure AT sukhvirsingh developmentofhematopoieticsyndromemicemodelforlocalizedradiationexposure AT sandeepkumarshukla developmentofhematopoieticsyndromemicemodelforlocalizedradiationexposure
_version_	1718387661539901440

Development of hematopoietic syndrome mice model for localized radiation exposure

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