A LysR-Type Transcriptional Regulator Controls Multiple Phenotypes in Acinetobacter baumannii

Acinetobacter baumannii is a multidrug-resistant, Gram-negative nosocomial pathogen that exhibits phenotypic heterogeneity resulting in virulent opaque (VIR-O) and avirulent translucent (AV-T) colony variants. Each variant has a distinct gene expression profile resulting in multiple phenotypic diffe...

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Autores principales:	Aimee R. P. Tierney, Chui Yoke Chin, David S. Weiss, Philip N. Rather
Formato:	article
Lenguaje:	EN
Publicado:	Frontiers Media S.A. 2021
Materias:	Acinetobacter baumannii AB5075 LysR-type transcriptional regulator phenotypic heterogeneity quorum sensing motility Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/d0f5a9cbf7ce4271860b95d9cd3ba0ec
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spelling	oai:doaj.org-article:d0f5a9cbf7ce4271860b95d9cd3ba0ec2021-11-04T05:15:40ZA LysR-Type Transcriptional Regulator Controls Multiple Phenotypes in Acinetobacter baumannii2235-298810.3389/fcimb.2021.778331https://doaj.org/article/d0f5a9cbf7ce4271860b95d9cd3ba0ec2021-11-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fcimb.2021.778331/fullhttps://doaj.org/toc/2235-2988Acinetobacter baumannii is a multidrug-resistant, Gram-negative nosocomial pathogen that exhibits phenotypic heterogeneity resulting in virulent opaque (VIR-O) and avirulent translucent (AV-T) colony variants. Each variant has a distinct gene expression profile resulting in multiple phenotypic differences. Cells interconvert between the VIR-O and AV-T variants at high frequency under laboratory conditions, suggesting that the genetic mechanism underlying the phenotypic switch could be manipulated to attenuate virulence. Therefore, our group has focused on identifying and characterizing genes that regulate this switch, which led to the investigation of ABUW_1132 (1132), a highly conserved gene predicted to encode a LysR-type transcriptional regulator. ABUW_1132 was shown to be a global regulator as the expression of 74 genes was altered ≥ 2-fold in an 1132 deletion mutant. The 1132 deletion also resulted in a 16-fold decrease in VIR-O to AV-T switching, loss of 3-OH-C12-HSL secretion, and reduced surface-associated motility. Further, the deletion of 1132 in the AV-T background caused elevated capsule production, which increased colony opacity and altered the typical avirulent phenotype of translucent cells. These findings distinguish 1132 as a global regulatory gene and advance our understanding of A. baumannii’s opacity-virulence switch.Aimee R. P. TierneyChui Yoke ChinChui Yoke ChinChui Yoke ChinChui Yoke ChinDavid S. WeissDavid S. WeissDavid S. WeissDavid S. WeissDavid S. WeissPhilip N. RatherPhilip N. RatherFrontiers Media S.A.articleAcinetobacter baumanniiAB5075LysR-type transcriptional regulatorphenotypic heterogeneityquorum sensingmotilityMicrobiologyQR1-502ENFrontiers in Cellular and Infection Microbiology, Vol 11 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Acinetobacter baumannii AB5075 LysR-type transcriptional regulator phenotypic heterogeneity quorum sensing motility Microbiology QR1-502
spellingShingle	Acinetobacter baumannii AB5075 LysR-type transcriptional regulator phenotypic heterogeneity quorum sensing motility Microbiology QR1-502 Aimee R. P. Tierney Chui Yoke Chin Chui Yoke Chin Chui Yoke Chin Chui Yoke Chin David S. Weiss David S. Weiss David S. Weiss David S. Weiss David S. Weiss Philip N. Rather Philip N. Rather A LysR-Type Transcriptional Regulator Controls Multiple Phenotypes in Acinetobacter baumannii
description	Acinetobacter baumannii is a multidrug-resistant, Gram-negative nosocomial pathogen that exhibits phenotypic heterogeneity resulting in virulent opaque (VIR-O) and avirulent translucent (AV-T) colony variants. Each variant has a distinct gene expression profile resulting in multiple phenotypic differences. Cells interconvert between the VIR-O and AV-T variants at high frequency under laboratory conditions, suggesting that the genetic mechanism underlying the phenotypic switch could be manipulated to attenuate virulence. Therefore, our group has focused on identifying and characterizing genes that regulate this switch, which led to the investigation of ABUW_1132 (1132), a highly conserved gene predicted to encode a LysR-type transcriptional regulator. ABUW_1132 was shown to be a global regulator as the expression of 74 genes was altered ≥ 2-fold in an 1132 deletion mutant. The 1132 deletion also resulted in a 16-fold decrease in VIR-O to AV-T switching, loss of 3-OH-C12-HSL secretion, and reduced surface-associated motility. Further, the deletion of 1132 in the AV-T background caused elevated capsule production, which increased colony opacity and altered the typical avirulent phenotype of translucent cells. These findings distinguish 1132 as a global regulatory gene and advance our understanding of A. baumannii’s opacity-virulence switch.
format	article
author	Aimee R. P. Tierney Chui Yoke Chin Chui Yoke Chin Chui Yoke Chin Chui Yoke Chin David S. Weiss David S. Weiss David S. Weiss David S. Weiss David S. Weiss Philip N. Rather Philip N. Rather
author_facet	Aimee R. P. Tierney Chui Yoke Chin Chui Yoke Chin Chui Yoke Chin Chui Yoke Chin David S. Weiss David S. Weiss David S. Weiss David S. Weiss David S. Weiss Philip N. Rather Philip N. Rather
author_sort	Aimee R. P. Tierney
title	A LysR-Type Transcriptional Regulator Controls Multiple Phenotypes in Acinetobacter baumannii
title_short	A LysR-Type Transcriptional Regulator Controls Multiple Phenotypes in Acinetobacter baumannii
title_full	A LysR-Type Transcriptional Regulator Controls Multiple Phenotypes in Acinetobacter baumannii
title_fullStr	A LysR-Type Transcriptional Regulator Controls Multiple Phenotypes in Acinetobacter baumannii
title_full_unstemmed	A LysR-Type Transcriptional Regulator Controls Multiple Phenotypes in Acinetobacter baumannii
title_sort	lysr-type transcriptional regulator controls multiple phenotypes in acinetobacter baumannii
publisher	Frontiers Media S.A.
publishDate	2021
url	https://doaj.org/article/d0f5a9cbf7ce4271860b95d9cd3ba0ec
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A LysR-Type Transcriptional Regulator Controls Multiple Phenotypes in Acinetobacter baumannii

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