Metabolomics improves the histopathological diagnosis of asphyxial deaths: an animal proof-of-concept model

Abstract The diagnosis of mechanical asphyxia remains one of the most difficult issues in forensic pathology. Asphyxia ultimately results in cardiac arrest (CA) and, as there are no specific markers, the differential diagnosis of primitive CA and CA secondary to asphyxiation relies on circumstantial...

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Autores principales:	Emanuela Locci, Alberto Chighine, Antonio Noto, Giulio Ferino, Alfonso Baldi, Dimitrios Varvarousis, Theodoros Xanthos, Fabio De-Giorgio, Matteo Stocchero, Ernesto d’Aloja
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/d0f6409f753c49d49c0025cf229d97fe
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spelling	oai:doaj.org-article:d0f6409f753c49d49c0025cf229d97fe2021-12-02T17:15:28ZMetabolomics improves the histopathological diagnosis of asphyxial deaths: an animal proof-of-concept model10.1038/s41598-021-89570-02045-2322https://doaj.org/article/d0f6409f753c49d49c0025cf229d97fe2021-05-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-89570-0https://doaj.org/toc/2045-2322Abstract The diagnosis of mechanical asphyxia remains one of the most difficult issues in forensic pathology. Asphyxia ultimately results in cardiac arrest (CA) and, as there are no specific markers, the differential diagnosis of primitive CA and CA secondary to asphyxiation relies on circumstantial details and on the pathologist experience, lacking objective evidence. Histological examination is currently considered the gold standard for CA post-mortem diagnosis. Here we present the comparative results of histopathology versus those previously obtained by 1H nuclear magnetic resonance (NMR) metabolomics in a swine model, originally designed for clinical purposes, exposed to two different CA causes, namely ventricular fibrillation and asphyxia. While heart and brain microscopical analysis could identify the damage induced by CA without providing any additional information on the CA cause, metabolomics allowed the identification of clearly different profiles between the two groups and showed major differences between asphyxiated animals with good and poor outcomes. Minute-by-minute plasma sampling allowed to associate these modifications to the pre-arrest asphyxial phase showing a clear correlation to the cellular effect of mechanical asphyxia reproduced in the experiment. The results suggest that metabolomics provides additional evidence beyond that obtained by histology and immunohistochemistry in the differential diagnosis of CA.Emanuela LocciAlberto ChighineAntonio NotoGiulio FerinoAlfonso BaldiDimitrios VarvarousisTheodoros XanthosFabio De-GiorgioMatteo StoccheroErnesto d’AlojaNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-11 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Emanuela Locci Alberto Chighine Antonio Noto Giulio Ferino Alfonso Baldi Dimitrios Varvarousis Theodoros Xanthos Fabio De-Giorgio Matteo Stocchero Ernesto d’Aloja Metabolomics improves the histopathological diagnosis of asphyxial deaths: an animal proof-of-concept model
description	Abstract The diagnosis of mechanical asphyxia remains one of the most difficult issues in forensic pathology. Asphyxia ultimately results in cardiac arrest (CA) and, as there are no specific markers, the differential diagnosis of primitive CA and CA secondary to asphyxiation relies on circumstantial details and on the pathologist experience, lacking objective evidence. Histological examination is currently considered the gold standard for CA post-mortem diagnosis. Here we present the comparative results of histopathology versus those previously obtained by 1H nuclear magnetic resonance (NMR) metabolomics in a swine model, originally designed for clinical purposes, exposed to two different CA causes, namely ventricular fibrillation and asphyxia. While heart and brain microscopical analysis could identify the damage induced by CA without providing any additional information on the CA cause, metabolomics allowed the identification of clearly different profiles between the two groups and showed major differences between asphyxiated animals with good and poor outcomes. Minute-by-minute plasma sampling allowed to associate these modifications to the pre-arrest asphyxial phase showing a clear correlation to the cellular effect of mechanical asphyxia reproduced in the experiment. The results suggest that metabolomics provides additional evidence beyond that obtained by histology and immunohistochemistry in the differential diagnosis of CA.
format	article
author	Emanuela Locci Alberto Chighine Antonio Noto Giulio Ferino Alfonso Baldi Dimitrios Varvarousis Theodoros Xanthos Fabio De-Giorgio Matteo Stocchero Ernesto d’Aloja
author_facet	Emanuela Locci Alberto Chighine Antonio Noto Giulio Ferino Alfonso Baldi Dimitrios Varvarousis Theodoros Xanthos Fabio De-Giorgio Matteo Stocchero Ernesto d’Aloja
author_sort	Emanuela Locci
title	Metabolomics improves the histopathological diagnosis of asphyxial deaths: an animal proof-of-concept model
title_short	Metabolomics improves the histopathological diagnosis of asphyxial deaths: an animal proof-of-concept model
title_full	Metabolomics improves the histopathological diagnosis of asphyxial deaths: an animal proof-of-concept model
title_fullStr	Metabolomics improves the histopathological diagnosis of asphyxial deaths: an animal proof-of-concept model
title_full_unstemmed	Metabolomics improves the histopathological diagnosis of asphyxial deaths: an animal proof-of-concept model
title_sort	metabolomics improves the histopathological diagnosis of asphyxial deaths: an animal proof-of-concept model
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/d0f6409f753c49d49c0025cf229d97fe
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Metabolomics improves the histopathological diagnosis of asphyxial deaths: an animal proof-of-concept model

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