Monitoring of cytogenetic effects in tick-borne encephalitis patients with type 2 diabetes mellitus, depending on polymorphism of glutathione-S-transferase genes

BACKGROUND: Tick-borne encephalitis (TBE) is an acute viral disease with activation of oxidative stress and increasing in cytogenetic instability. Clinical symptoms of infectious diseases usually more severe in patients with type 2 diabetes mellitus (DM2), especially in the case of burden in the gen...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Nikolay N. Ilyinskikh, Marina S. Kostromeeva, Ekaterina N. Ilyinskikh
Formato:	article
Lenguaje:	EN RU
Publicado:	Endocrinology Research Centre 2019
Materias:	type 2 diabetes mellitus tick-borne encephalitis micronucleus assay buccal cells gstm1 gstt1 glutathione-s-transferase Nutritional diseases. Deficiency diseases RC620-627
Acceso en línea:	https://doaj.org/article/d0f8409380ae472696345d6bdf98e726
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:d0f8409380ae472696345d6bdf98e726
record_format	dspace
spelling	oai:doaj.org-article:d0f8409380ae472696345d6bdf98e7262021-11-14T09:00:22ZMonitoring of cytogenetic effects in tick-borne encephalitis patients with type 2 diabetes mellitus, depending on polymorphism of glutathione-S-transferase genes2072-03512072-037810.14341/DM9715https://doaj.org/article/d0f8409380ae472696345d6bdf98e7262019-08-01T00:00:00Zhttps://www.dia-endojournals.ru/jour/article/view/9715https://doaj.org/toc/2072-0351https://doaj.org/toc/2072-0378BACKGROUND: Tick-borne encephalitis (TBE) is an acute viral disease with activation of oxidative stress and increasing in cytogenetic instability. Clinical symptoms of infectious diseases usually more severe in patients with type 2 diabetes mellitus (DM2), especially in the case of burden in the genotype of mutant variants of glutathione-S-transferase genes GSTM1 and GSTT1. AIMS: The aim of this study was to study the dynamics of the frequency of micronucleated cells in patients with acute TBE with concomitant DM2, depending on the burden of active and inactive variants of glutathione-S-transferase genes (GSTM1 and GSTT1) in the patient’s genotype. MATERIALS AND METHODS: Totally, samples to make micronucleus assay were obtained from 138 patients with febrile illness of acute TBE, 64 of whom were diagnosed with concomitant DM2 (groups 3 and 4). As control groups, 57 healthy individuals (control 1) and 61 patients with DM2 (control 2) were examined. The samples of buccal cells for the micronucleus assay were repeatedly obtained from the individuals on the first day of admission, and also after 1 week, 1, 3 and 6 months. Polymerase chain reaction was used to analyze the variants of the GSTM1 and GSTT1 genes. RESULTS: On the first days of the disease, significant increases in the frequency of micronucleated buccal cells were determined in all TBE patients as compared to controls 1 and 2 (P<0.001). Significant increases in the frequency of micronucleated buccal cells was revealed in groups 3 and 4 of the TBE patients who were carriers of inactive variants of the GSTM1(0) and GSTT1(0) genes, as compared to the subgroup of TBE patients with active variants of these genes (P<0.001). In all subgroups of TBE patients with concomitant DM2, the frequencies of micronucleated cells were significantly higher than in the subgroups of TBE patients without DM2 (P<0.001). Study of the dynamics of the frequency of micronucleated buccal cells, as compared to the control, demonstrated that the highest and long-lasting (within 6 months) cytogenetical effects were maintained in the group of TBE patients with genotype GSTM1(0)/GSTT1 (0) and concomitant DM2. CONCLUSION: The most prolonged and highest increases in the frequencies of cytogenetically instable cells were determined in the group of acute TBE patients with concomitant DM2 who were carriers of the genotype with inactive variants of both GSTM1(0) and GSTT1(0) glutathione-S-transferase genes.Nikolay N. IlyinskikhMarina S. KostromeevaEkaterina N. IlyinskikhEndocrinology Research Centrearticletype 2 diabetes mellitustick-borne encephalitismicronucleus assaybuccal cellsgstm1gstt1glutathione-s-transferaseNutritional diseases. Deficiency diseasesRC620-627ENRUСахарный диабет, Vol 22, Iss 3, Pp 225-232 (2019)
institution	DOAJ
collection	DOAJ
language	EN RU
topic	type 2 diabetes mellitus tick-borne encephalitis micronucleus assay buccal cells gstm1 gstt1 glutathione-s-transferase Nutritional diseases. Deficiency diseases RC620-627
spellingShingle	type 2 diabetes mellitus tick-borne encephalitis micronucleus assay buccal cells gstm1 gstt1 glutathione-s-transferase Nutritional diseases. Deficiency diseases RC620-627 Nikolay N. Ilyinskikh Marina S. Kostromeeva Ekaterina N. Ilyinskikh Monitoring of cytogenetic effects in tick-borne encephalitis patients with type 2 diabetes mellitus, depending on polymorphism of glutathione-S-transferase genes
description	BACKGROUND: Tick-borne encephalitis (TBE) is an acute viral disease with activation of oxidative stress and increasing in cytogenetic instability. Clinical symptoms of infectious diseases usually more severe in patients with type 2 diabetes mellitus (DM2), especially in the case of burden in the genotype of mutant variants of glutathione-S-transferase genes GSTM1 and GSTT1. AIMS: The aim of this study was to study the dynamics of the frequency of micronucleated cells in patients with acute TBE with concomitant DM2, depending on the burden of active and inactive variants of glutathione-S-transferase genes (GSTM1 and GSTT1) in the patient’s genotype. MATERIALS AND METHODS: Totally, samples to make micronucleus assay were obtained from 138 patients with febrile illness of acute TBE, 64 of whom were diagnosed with concomitant DM2 (groups 3 and 4). As control groups, 57 healthy individuals (control 1) and 61 patients with DM2 (control 2) were examined. The samples of buccal cells for the micronucleus assay were repeatedly obtained from the individuals on the first day of admission, and also after 1 week, 1, 3 and 6 months. Polymerase chain reaction was used to analyze the variants of the GSTM1 and GSTT1 genes. RESULTS: On the first days of the disease, significant increases in the frequency of micronucleated buccal cells were determined in all TBE patients as compared to controls 1 and 2 (P<0.001). Significant increases in the frequency of micronucleated buccal cells was revealed in groups 3 and 4 of the TBE patients who were carriers of inactive variants of the GSTM1(0) and GSTT1(0) genes, as compared to the subgroup of TBE patients with active variants of these genes (P<0.001). In all subgroups of TBE patients with concomitant DM2, the frequencies of micronucleated cells were significantly higher than in the subgroups of TBE patients without DM2 (P<0.001). Study of the dynamics of the frequency of micronucleated buccal cells, as compared to the control, demonstrated that the highest and long-lasting (within 6 months) cytogenetical effects were maintained in the group of TBE patients with genotype GSTM1(0)/GSTT1 (0) and concomitant DM2. CONCLUSION: The most prolonged and highest increases in the frequencies of cytogenetically instable cells were determined in the group of acute TBE patients with concomitant DM2 who were carriers of the genotype with inactive variants of both GSTM1(0) and GSTT1(0) glutathione-S-transferase genes.
format	article
author	Nikolay N. Ilyinskikh Marina S. Kostromeeva Ekaterina N. Ilyinskikh
author_facet	Nikolay N. Ilyinskikh Marina S. Kostromeeva Ekaterina N. Ilyinskikh
author_sort	Nikolay N. Ilyinskikh
title	Monitoring of cytogenetic effects in tick-borne encephalitis patients with type 2 diabetes mellitus, depending on polymorphism of glutathione-S-transferase genes
title_short	Monitoring of cytogenetic effects in tick-borne encephalitis patients with type 2 diabetes mellitus, depending on polymorphism of glutathione-S-transferase genes
title_full	Monitoring of cytogenetic effects in tick-borne encephalitis patients with type 2 diabetes mellitus, depending on polymorphism of glutathione-S-transferase genes
title_fullStr	Monitoring of cytogenetic effects in tick-borne encephalitis patients with type 2 diabetes mellitus, depending on polymorphism of glutathione-S-transferase genes
title_full_unstemmed	Monitoring of cytogenetic effects in tick-borne encephalitis patients with type 2 diabetes mellitus, depending on polymorphism of glutathione-S-transferase genes
title_sort	monitoring of cytogenetic effects in tick-borne encephalitis patients with type 2 diabetes mellitus, depending on polymorphism of glutathione-s-transferase genes
publisher	Endocrinology Research Centre
publishDate	2019
url	https://doaj.org/article/d0f8409380ae472696345d6bdf98e726
work_keys_str_mv	AT nikolaynilyinskikh monitoringofcytogeneticeffectsintickborneencephalitispatientswithtype2diabetesmellitusdependingonpolymorphismofglutathionestransferasegenes AT marinaskostromeeva monitoringofcytogeneticeffectsintickborneencephalitispatientswithtype2diabetesmellitusdependingonpolymorphismofglutathionestransferasegenes AT ekaterinanilyinskikh monitoringofcytogeneticeffectsintickborneencephalitispatientswithtype2diabetesmellitusdependingonpolymorphismofglutathionestransferasegenes
_version_	1718429492583596032

Monitoring of cytogenetic effects in tick-borne encephalitis patients with type 2 diabetes mellitus, depending on polymorphism of glutathione-S-transferase genes

Ejemplares similares