Establishment and characterization of immortalized human breast cancer cell lines from breast cancer patient-derived xenografts (PDX)

Abstract The application of patient-derived xenografts (PDX) in drug screening and testing is a costly and time-consuming endeavor. While cell lines permit extensive mechanistic studies, many human breast cancer cell lines lack patient characteristics and clinical treatment information. Establishing...

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Autores principales:	Yongxian Zhuang, Jordan M. Grainger, Peter T. Vedell, Jia Yu, Ann M. Moyer, Huanyao Gao, Xiao-Yang Fan, Sisi Qin, Duan Liu, Krishna R. Kalari, Matthew P. Goetz, Judy C. Boughey, Richard M. Weinshilboum, Liewei Wang
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Neoplasms. Tumors. Oncology. Including cancer and carcinogens RC254-282
Acceso en línea:	https://doaj.org/article/d0faaa823e1d474ca427db78a9f24251
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spelling	oai:doaj.org-article:d0faaa823e1d474ca427db78a9f242512021-12-02T17:40:31ZEstablishment and characterization of immortalized human breast cancer cell lines from breast cancer patient-derived xenografts (PDX)10.1038/s41523-021-00285-x2374-4677https://doaj.org/article/d0faaa823e1d474ca427db78a9f242512021-06-01T00:00:00Zhttps://doi.org/10.1038/s41523-021-00285-xhttps://doaj.org/toc/2374-4677Abstract The application of patient-derived xenografts (PDX) in drug screening and testing is a costly and time-consuming endeavor. While cell lines permit extensive mechanistic studies, many human breast cancer cell lines lack patient characteristics and clinical treatment information. Establishing cell lines that retain patient’s genetic and drug response information would enable greater drug screening and mechanistic studies. Therefore, we utilized breast cancer PDX from the Mayo Breast Cancer Genome Guided Therapy Study (BEAUTY) to establish two immortalized, genomically unique breast cancer cell lines. Through extensive genetic and therapeutic testing, the cell lines were found to retain the same clinical subtype, major somatic alterations, and drug response phenotypes as their corresponding PDX and patient tumor. Our findings demonstrate PDX can be utilized to develop immortalized breast cancer cell lines and provide a valuable tool for understanding the molecular mechanism of drug resistance and exploring novel treatment strategies.Yongxian ZhuangJordan M. GraingerPeter T. VedellJia YuAnn M. MoyerHuanyao GaoXiao-Yang FanSisi QinDuan LiuKrishna R. KalariMatthew P. GoetzJudy C. BougheyRichard M. WeinshilboumLiewei WangNature PortfolioarticleNeoplasms. Tumors. Oncology. Including cancer and carcinogensRC254-282ENnpj Breast Cancer, Vol 7, Iss 1, Pp 1-12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Neoplasms. Tumors. Oncology. Including cancer and carcinogens RC254-282
spellingShingle	Neoplasms. Tumors. Oncology. Including cancer and carcinogens RC254-282 Yongxian Zhuang Jordan M. Grainger Peter T. Vedell Jia Yu Ann M. Moyer Huanyao Gao Xiao-Yang Fan Sisi Qin Duan Liu Krishna R. Kalari Matthew P. Goetz Judy C. Boughey Richard M. Weinshilboum Liewei Wang Establishment and characterization of immortalized human breast cancer cell lines from breast cancer patient-derived xenografts (PDX)
description	Abstract The application of patient-derived xenografts (PDX) in drug screening and testing is a costly and time-consuming endeavor. While cell lines permit extensive mechanistic studies, many human breast cancer cell lines lack patient characteristics and clinical treatment information. Establishing cell lines that retain patient’s genetic and drug response information would enable greater drug screening and mechanistic studies. Therefore, we utilized breast cancer PDX from the Mayo Breast Cancer Genome Guided Therapy Study (BEAUTY) to establish two immortalized, genomically unique breast cancer cell lines. Through extensive genetic and therapeutic testing, the cell lines were found to retain the same clinical subtype, major somatic alterations, and drug response phenotypes as their corresponding PDX and patient tumor. Our findings demonstrate PDX can be utilized to develop immortalized breast cancer cell lines and provide a valuable tool for understanding the molecular mechanism of drug resistance and exploring novel treatment strategies.
format	article
author	Yongxian Zhuang Jordan M. Grainger Peter T. Vedell Jia Yu Ann M. Moyer Huanyao Gao Xiao-Yang Fan Sisi Qin Duan Liu Krishna R. Kalari Matthew P. Goetz Judy C. Boughey Richard M. Weinshilboum Liewei Wang
author_facet	Yongxian Zhuang Jordan M. Grainger Peter T. Vedell Jia Yu Ann M. Moyer Huanyao Gao Xiao-Yang Fan Sisi Qin Duan Liu Krishna R. Kalari Matthew P. Goetz Judy C. Boughey Richard M. Weinshilboum Liewei Wang
author_sort	Yongxian Zhuang
title	Establishment and characterization of immortalized human breast cancer cell lines from breast cancer patient-derived xenografts (PDX)
title_short	Establishment and characterization of immortalized human breast cancer cell lines from breast cancer patient-derived xenografts (PDX)
title_full	Establishment and characterization of immortalized human breast cancer cell lines from breast cancer patient-derived xenografts (PDX)
title_fullStr	Establishment and characterization of immortalized human breast cancer cell lines from breast cancer patient-derived xenografts (PDX)
title_full_unstemmed	Establishment and characterization of immortalized human breast cancer cell lines from breast cancer patient-derived xenografts (PDX)
title_sort	establishment and characterization of immortalized human breast cancer cell lines from breast cancer patient-derived xenografts (pdx)
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/d0faaa823e1d474ca427db78a9f24251
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Establishment and characterization of immortalized human breast cancer cell lines from breast cancer patient-derived xenografts (PDX)

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