Metagenomics analysis of bacteriophages and antimicrobial resistance from global urban sewage

Abstract Bacteriophages, or phages, are ubiquitous bacterial and archaeal viruses with an estimated total global population of 1031. It is well-known that wherever there are bacteria, their phage counterparts will be found, aiding in shaping the bacterial population. The present study used metagenom...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Josephine E. S. Strange, Pimlapas Leekitcharoenphon, Frederik Duus Møller, Frank M. Aarestrup
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/d0fefe028d884484ba1bc42075b8f872
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:d0fefe028d884484ba1bc42075b8f872
record_format	dspace
spelling	oai:doaj.org-article:d0fefe028d884484ba1bc42075b8f8722021-12-02T14:12:40ZMetagenomics analysis of bacteriophages and antimicrobial resistance from global urban sewage10.1038/s41598-021-80990-62045-2322https://doaj.org/article/d0fefe028d884484ba1bc42075b8f8722021-01-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-80990-6https://doaj.org/toc/2045-2322Abstract Bacteriophages, or phages, are ubiquitous bacterial and archaeal viruses with an estimated total global population of 1031. It is well-known that wherever there are bacteria, their phage counterparts will be found, aiding in shaping the bacterial population. The present study used metagenomic data from global influent sewage in 79 cities in 60 countries to identify phages associated with bacteria and to explore their potential role in antimicrobial resistance gene (ARG) dissemination. The reads were mapped to known databases for bacteriophages and their abundances determined and correlated to geographic origin and the countries socio-economic status, as well as the abundances of bacterial species and ARG. We found that some phages were not equally distributed on a global scale, but their distribution was rather dictated by region and the socioeconomic status of the specific countries. This study provides a preliminary insight into the global and regional distribution of phages and their potential impact on the transmission of ARGs between bacteria. Moreover, the findings may indicate that phages in sewage could have adopted a lytic lifestyle, meaning that most may not be associated with bacteria and instead may be widely distributed as free-living phages, which are known to persist longer in the environment than their hosts. In addition, a significant correlation between phages and ARGs was obtained, indicating that phages may play a role in ARG dissemination. However, further analyses are needed to establish the true relationship between phages and ARGs due to a low abundance of the phages identified.Josephine E. S. StrangePimlapas LeekitcharoenphonFrederik Duus MøllerFrank M. AarestrupNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-11 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Josephine E. S. Strange Pimlapas Leekitcharoenphon Frederik Duus Møller Frank M. Aarestrup Metagenomics analysis of bacteriophages and antimicrobial resistance from global urban sewage
description	Abstract Bacteriophages, or phages, are ubiquitous bacterial and archaeal viruses with an estimated total global population of 1031. It is well-known that wherever there are bacteria, their phage counterparts will be found, aiding in shaping the bacterial population. The present study used metagenomic data from global influent sewage in 79 cities in 60 countries to identify phages associated with bacteria and to explore their potential role in antimicrobial resistance gene (ARG) dissemination. The reads were mapped to known databases for bacteriophages and their abundances determined and correlated to geographic origin and the countries socio-economic status, as well as the abundances of bacterial species and ARG. We found that some phages were not equally distributed on a global scale, but their distribution was rather dictated by region and the socioeconomic status of the specific countries. This study provides a preliminary insight into the global and regional distribution of phages and their potential impact on the transmission of ARGs between bacteria. Moreover, the findings may indicate that phages in sewage could have adopted a lytic lifestyle, meaning that most may not be associated with bacteria and instead may be widely distributed as free-living phages, which are known to persist longer in the environment than their hosts. In addition, a significant correlation between phages and ARGs was obtained, indicating that phages may play a role in ARG dissemination. However, further analyses are needed to establish the true relationship between phages and ARGs due to a low abundance of the phages identified.
format	article
author	Josephine E. S. Strange Pimlapas Leekitcharoenphon Frederik Duus Møller Frank M. Aarestrup
author_facet	Josephine E. S. Strange Pimlapas Leekitcharoenphon Frederik Duus Møller Frank M. Aarestrup
author_sort	Josephine E. S. Strange
title	Metagenomics analysis of bacteriophages and antimicrobial resistance from global urban sewage
title_short	Metagenomics analysis of bacteriophages and antimicrobial resistance from global urban sewage
title_full	Metagenomics analysis of bacteriophages and antimicrobial resistance from global urban sewage
title_fullStr	Metagenomics analysis of bacteriophages and antimicrobial resistance from global urban sewage
title_full_unstemmed	Metagenomics analysis of bacteriophages and antimicrobial resistance from global urban sewage
title_sort	metagenomics analysis of bacteriophages and antimicrobial resistance from global urban sewage
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/d0fefe028d884484ba1bc42075b8f872
work_keys_str_mv	AT josephineesstrange metagenomicsanalysisofbacteriophagesandantimicrobialresistancefromglobalurbansewage AT pimlapasleekitcharoenphon metagenomicsanalysisofbacteriophagesandantimicrobialresistancefromglobalurbansewage AT frederikduusmøller metagenomicsanalysisofbacteriophagesandantimicrobialresistancefromglobalurbansewage AT frankmaarestrup metagenomicsanalysisofbacteriophagesandantimicrobialresistancefromglobalurbansewage
_version_	1718391813353504768

Metagenomics analysis of bacteriophages and antimicrobial resistance from global urban sewage

Ejemplares similares