Pasteurella multocida capsular: lipopolysaccharide types D:L6 and A:L3 remain to be the main epidemic genotypes of pigs in China

Abstract Pasteurella multocida is a leading cause of respiratory disorders in pigs. This study was designed to understand the genotypical and antimicrobial resistant characteristics of P. multocida from pigs in China. To achieve this, we briefly investigated 158 P. multocida isolates from pigs with...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Songtao Liu, Lin Lin, Hao Yang, Wenqing Wu, Long Guo, Yue Zhang, Fei Wang, Xueying Wang, Wenbo Song, Lin Hua, Wan Liang, Xibiao Tang, Huanchun Chen, Zhong Peng, Bin Wu
Formato:	article
Lenguaje:	EN
Publicado:	BMC 2021
Materias:	Pasteurella multocida Capsular and LPS genotypes Virulence factor-encoding genes Antimicrobial susceptibility Pigs China Veterinary medicine SF600-1100 Public aspects of medicine RA1-1270
Acceso en línea:	https://doaj.org/article/d1047a5a22ca43719c6c76ef65ba2c53
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:d1047a5a22ca43719c6c76ef65ba2c53
record_format	dspace
spelling	oai:doaj.org-article:d1047a5a22ca43719c6c76ef65ba2c532021-11-07T12:03:52ZPasteurella multocida capsular: lipopolysaccharide types D:L6 and A:L3 remain to be the main epidemic genotypes of pigs in China10.1186/s44149-021-00031-72731-0442https://doaj.org/article/d1047a5a22ca43719c6c76ef65ba2c532021-11-01T00:00:00Zhttps://doi.org/10.1186/s44149-021-00031-7https://doaj.org/toc/2731-0442Abstract Pasteurella multocida is a leading cause of respiratory disorders in pigs. This study was designed to understand the genotypical and antimicrobial resistant characteristics of P. multocida from pigs in China. To achieve this, we briefly investigated 158 P. multocida isolates from pigs with respiratory disorders in China between 2019 and 2020. Genotyping through multiplex PCR assays assigned these 158 isolates into capsular genotypes A (60.13%, 95/158), D (35.44%, 56/158), F (4.43%, 7/158), and/or lipopolysaccharide (LPS) genotypes L3 (28.48%, 45/158) and L6 (66.46%, 105/158). In addition, eight isolates (5.06%, 8/158) were found to be nontypable using the LPS genotyping method. When combining the capsular genotypes and the LPS genotypes, D: L6 (34.81%, 55/158) and A: L6 (31.65%, 50/158) were the predominant genotypes, followed by A: L3 (24.05%, 38/158). PCR detection of virulence factor-encoding genes showed that over 80% of the isolates were positive for exbB, tonB, exbD, ompH, ptfA, fimA, sodA, sodC, fur, ompA, oma87, plpB, hsf-2, nanH and hgbB, suggesting the presence of these genes were broad characteristics of P. multocida. We also found approximately 63.92% (101/158), 51.27% (81/158), 8.86% (14/158), 7.59% (12/158), 3.16% (5/158), 0.63% (1/158), and 0.63% (1/158) of the isolates grew well in media with the presence of colistin (4 μg/mL), tetracycline (16 μg/mL), tigecycline (1 μg/mL), ampicillin (32 μg/mL), chloramphenicol (32 μg/mL), cefepime (16 μg/mL), and ciprofloxacin (1 μg/mL), respectively. This study contributes to the understanding of genotypes and antimicrobial resistance profile of P. multocida currently circulation in pigs of China.Songtao LiuLin LinHao YangWenqing WuLong GuoYue ZhangFei WangXueying WangWenbo SongLin HuaWan LiangXibiao TangHuanchun ChenZhong PengBin WuBMCarticlePasteurella multocidaCapsular and LPS genotypesVirulence factor-encoding genesAntimicrobial susceptibilityPigsChinaVeterinary medicineSF600-1100Public aspects of medicineRA1-1270ENAnimal Diseases, Vol 1, Iss 1, Pp 1-6 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Pasteurella multocida Capsular and LPS genotypes Virulence factor-encoding genes Antimicrobial susceptibility Pigs China Veterinary medicine SF600-1100 Public aspects of medicine RA1-1270
spellingShingle	Pasteurella multocida Capsular and LPS genotypes Virulence factor-encoding genes Antimicrobial susceptibility Pigs China Veterinary medicine SF600-1100 Public aspects of medicine RA1-1270 Songtao Liu Lin Lin Hao Yang Wenqing Wu Long Guo Yue Zhang Fei Wang Xueying Wang Wenbo Song Lin Hua Wan Liang Xibiao Tang Huanchun Chen Zhong Peng Bin Wu Pasteurella multocida capsular: lipopolysaccharide types D:L6 and A:L3 remain to be the main epidemic genotypes of pigs in China
description	Abstract Pasteurella multocida is a leading cause of respiratory disorders in pigs. This study was designed to understand the genotypical and antimicrobial resistant characteristics of P. multocida from pigs in China. To achieve this, we briefly investigated 158 P. multocida isolates from pigs with respiratory disorders in China between 2019 and 2020. Genotyping through multiplex PCR assays assigned these 158 isolates into capsular genotypes A (60.13%, 95/158), D (35.44%, 56/158), F (4.43%, 7/158), and/or lipopolysaccharide (LPS) genotypes L3 (28.48%, 45/158) and L6 (66.46%, 105/158). In addition, eight isolates (5.06%, 8/158) were found to be nontypable using the LPS genotyping method. When combining the capsular genotypes and the LPS genotypes, D: L6 (34.81%, 55/158) and A: L6 (31.65%, 50/158) were the predominant genotypes, followed by A: L3 (24.05%, 38/158). PCR detection of virulence factor-encoding genes showed that over 80% of the isolates were positive for exbB, tonB, exbD, ompH, ptfA, fimA, sodA, sodC, fur, ompA, oma87, plpB, hsf-2, nanH and hgbB, suggesting the presence of these genes were broad characteristics of P. multocida. We also found approximately 63.92% (101/158), 51.27% (81/158), 8.86% (14/158), 7.59% (12/158), 3.16% (5/158), 0.63% (1/158), and 0.63% (1/158) of the isolates grew well in media with the presence of colistin (4 μg/mL), tetracycline (16 μg/mL), tigecycline (1 μg/mL), ampicillin (32 μg/mL), chloramphenicol (32 μg/mL), cefepime (16 μg/mL), and ciprofloxacin (1 μg/mL), respectively. This study contributes to the understanding of genotypes and antimicrobial resistance profile of P. multocida currently circulation in pigs of China.
format	article
author	Songtao Liu Lin Lin Hao Yang Wenqing Wu Long Guo Yue Zhang Fei Wang Xueying Wang Wenbo Song Lin Hua Wan Liang Xibiao Tang Huanchun Chen Zhong Peng Bin Wu
author_facet	Songtao Liu Lin Lin Hao Yang Wenqing Wu Long Guo Yue Zhang Fei Wang Xueying Wang Wenbo Song Lin Hua Wan Liang Xibiao Tang Huanchun Chen Zhong Peng Bin Wu
author_sort	Songtao Liu
title	Pasteurella multocida capsular: lipopolysaccharide types D:L6 and A:L3 remain to be the main epidemic genotypes of pigs in China
title_short	Pasteurella multocida capsular: lipopolysaccharide types D:L6 and A:L3 remain to be the main epidemic genotypes of pigs in China
title_full	Pasteurella multocida capsular: lipopolysaccharide types D:L6 and A:L3 remain to be the main epidemic genotypes of pigs in China
title_fullStr	Pasteurella multocida capsular: lipopolysaccharide types D:L6 and A:L3 remain to be the main epidemic genotypes of pigs in China
title_full_unstemmed	Pasteurella multocida capsular: lipopolysaccharide types D:L6 and A:L3 remain to be the main epidemic genotypes of pigs in China
title_sort	pasteurella multocida capsular: lipopolysaccharide types d:l6 and a:l3 remain to be the main epidemic genotypes of pigs in china
publisher	BMC
publishDate	2021
url	https://doaj.org/article/d1047a5a22ca43719c6c76ef65ba2c53
work_keys_str_mv	AT songtaoliu pasteurellamultocidacapsularlipopolysaccharidetypesdl6andal3remaintobethemainepidemicgenotypesofpigsinchina AT linlin pasteurellamultocidacapsularlipopolysaccharidetypesdl6andal3remaintobethemainepidemicgenotypesofpigsinchina AT haoyang pasteurellamultocidacapsularlipopolysaccharidetypesdl6andal3remaintobethemainepidemicgenotypesofpigsinchina AT wenqingwu pasteurellamultocidacapsularlipopolysaccharidetypesdl6andal3remaintobethemainepidemicgenotypesofpigsinchina AT longguo pasteurellamultocidacapsularlipopolysaccharidetypesdl6andal3remaintobethemainepidemicgenotypesofpigsinchina AT yuezhang pasteurellamultocidacapsularlipopolysaccharidetypesdl6andal3remaintobethemainepidemicgenotypesofpigsinchina AT feiwang pasteurellamultocidacapsularlipopolysaccharidetypesdl6andal3remaintobethemainepidemicgenotypesofpigsinchina AT xueyingwang pasteurellamultocidacapsularlipopolysaccharidetypesdl6andal3remaintobethemainepidemicgenotypesofpigsinchina AT wenbosong pasteurellamultocidacapsularlipopolysaccharidetypesdl6andal3remaintobethemainepidemicgenotypesofpigsinchina AT linhua pasteurellamultocidacapsularlipopolysaccharidetypesdl6andal3remaintobethemainepidemicgenotypesofpigsinchina AT wanliang pasteurellamultocidacapsularlipopolysaccharidetypesdl6andal3remaintobethemainepidemicgenotypesofpigsinchina AT xibiaotang pasteurellamultocidacapsularlipopolysaccharidetypesdl6andal3remaintobethemainepidemicgenotypesofpigsinchina AT huanchunchen pasteurellamultocidacapsularlipopolysaccharidetypesdl6andal3remaintobethemainepidemicgenotypesofpigsinchina AT zhongpeng pasteurellamultocidacapsularlipopolysaccharidetypesdl6andal3remaintobethemainepidemicgenotypesofpigsinchina AT binwu pasteurellamultocidacapsularlipopolysaccharidetypesdl6andal3remaintobethemainepidemicgenotypesofpigsinchina
_version_	1718443577191694336

Pasteurella multocida capsular: lipopolysaccharide types D:L6 and A:L3 remain to be the main epidemic genotypes of pigs in China

Ejemplares similares