Efficient delivery of Notch1 siRNA to SKOV3 cells by cationic cholesterol derivative-based liposome

Yun-Chun Zhao,1 Li Zhang,2 Shi-Sen Feng,3 Lu Hong,3 Hai-Li Zheng,3 Li-Li Chen,4 Xiao-Ling Zheng,1 Yi-Qing Ye,1 Meng-Dan Zhao,1 Wen-Xi Wang,3 Cai-Hong Zheng1 1Pharmacy Department, Women’s Hospital, 2Pharmacy Department, The Second Affiliated Hospital, School of Medicine, Zhejiang Universit...

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Autores principales: Zhao Y, Zhang L, Feng S, Hong L, Zheng H, Chen L, Zheng X, Ye Y, Zhao M, Wang W, Zheng C
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Lenguaje:EN
Publicado: Dove Medical Press 2016
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spelling oai:doaj.org-article:d111ead5713e44dcb9db23fe8276f80c2021-12-02T02:28:07ZEfficient delivery of Notch1 siRNA to SKOV3 cells by cationic cholesterol derivative-based liposome1178-2013https://doaj.org/article/d111ead5713e44dcb9db23fe8276f80c2016-10-01T00:00:00Zhttps://www.dovepress.com/efficient-delivery-of-notch1-sirna-to-skov3-cells-by-cationic-choleste-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Yun-Chun Zhao,1 Li Zhang,2 Shi-Sen Feng,3 Lu Hong,3 Hai-Li Zheng,3 Li-Li Chen,4 Xiao-Ling Zheng,1 Yi-Qing Ye,1 Meng-Dan Zhao,1 Wen-Xi Wang,3 Cai-Hong Zheng1 1Pharmacy Department, Women’s Hospital, 2Pharmacy Department, The Second Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou, People’s Republic of China; 3Department of Pharmaceutic Preparation, College of Pharmaceutical Science, Zhejiang University of Technology, Hangzhou, 4Department of Gynecologic Oncology, Women’s Hospital, School of Medicine, Zhejiang University, Hangzhou, People’s Republic of China Abstract: A novel cationic cholesterol derivative-based small interfering RNA (siRNA) interference strategy was suggested to inhibit Notch1 activation in SKOV3 cells for the gene therapy of ovarian cancer. The cationic cholesterol derivative, N-(cholesterylhemisuccinoyl-amino-3-propyl)-N, N-dimethylamine (DMAPA-chems) liposome, was incubated with siRNA at different nitrogen-to-phosphate ratios to form stabilized, near-spherical siRNA/DMAPA-chems nanoparticles with sizes of 100–200 nm and zeta potentials of 40–50 mV. The siRNA/DMAPA-chems nanoparticles protected siRNA from nuclease degradation in 25% fetal bovine serum. The nanoparticles exhibited high cell uptake and Notch1 gene knockdown efficiency in SKOV3 cells at an nitrogen-to-phosphate ratio of 100 and an siRNA concentration of 50 nM. They also inhibited the growth and promoted the apoptosis of SKOV3 cells. These results may provide the potential for using cationic cholesterol derivatives as efficient nonviral siRNA carriers for the suppression of Notch1 activation in ovarian cancer cells. Keywords: siRNA, cationic cholesterol derivative, Notch1, ovarian cancer cellsZhao YZhang LFeng SHong LZheng HChen LZheng XYe YZhao MWang WZheng CDove Medical PressarticlesiRNAcationic cholesterol derivativeNotch1ovarian cancer cellsMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol Volume 11, Pp 5485-5496 (2016)
institution DOAJ
collection DOAJ
language EN
topic siRNA
cationic cholesterol derivative
Notch1
ovarian cancer cells
Medicine (General)
R5-920
spellingShingle siRNA
cationic cholesterol derivative
Notch1
ovarian cancer cells
Medicine (General)
R5-920
Zhao Y
Zhang L
Feng S
Hong L
Zheng H
Chen L
Zheng X
Ye Y
Zhao M
Wang W
Zheng C
Efficient delivery of Notch1 siRNA to SKOV3 cells by cationic cholesterol derivative-based liposome
description Yun-Chun Zhao,1 Li Zhang,2 Shi-Sen Feng,3 Lu Hong,3 Hai-Li Zheng,3 Li-Li Chen,4 Xiao-Ling Zheng,1 Yi-Qing Ye,1 Meng-Dan Zhao,1 Wen-Xi Wang,3 Cai-Hong Zheng1 1Pharmacy Department, Women’s Hospital, 2Pharmacy Department, The Second Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou, People’s Republic of China; 3Department of Pharmaceutic Preparation, College of Pharmaceutical Science, Zhejiang University of Technology, Hangzhou, 4Department of Gynecologic Oncology, Women’s Hospital, School of Medicine, Zhejiang University, Hangzhou, People’s Republic of China Abstract: A novel cationic cholesterol derivative-based small interfering RNA (siRNA) interference strategy was suggested to inhibit Notch1 activation in SKOV3 cells for the gene therapy of ovarian cancer. The cationic cholesterol derivative, N-(cholesterylhemisuccinoyl-amino-3-propyl)-N, N-dimethylamine (DMAPA-chems) liposome, was incubated with siRNA at different nitrogen-to-phosphate ratios to form stabilized, near-spherical siRNA/DMAPA-chems nanoparticles with sizes of 100–200 nm and zeta potentials of 40–50 mV. The siRNA/DMAPA-chems nanoparticles protected siRNA from nuclease degradation in 25% fetal bovine serum. The nanoparticles exhibited high cell uptake and Notch1 gene knockdown efficiency in SKOV3 cells at an nitrogen-to-phosphate ratio of 100 and an siRNA concentration of 50 nM. They also inhibited the growth and promoted the apoptosis of SKOV3 cells. These results may provide the potential for using cationic cholesterol derivatives as efficient nonviral siRNA carriers for the suppression of Notch1 activation in ovarian cancer cells. Keywords: siRNA, cationic cholesterol derivative, Notch1, ovarian cancer cells
format article
author Zhao Y
Zhang L
Feng S
Hong L
Zheng H
Chen L
Zheng X
Ye Y
Zhao M
Wang W
Zheng C
author_facet Zhao Y
Zhang L
Feng S
Hong L
Zheng H
Chen L
Zheng X
Ye Y
Zhao M
Wang W
Zheng C
author_sort Zhao Y
title Efficient delivery of Notch1 siRNA to SKOV3 cells by cationic cholesterol derivative-based liposome
title_short Efficient delivery of Notch1 siRNA to SKOV3 cells by cationic cholesterol derivative-based liposome
title_full Efficient delivery of Notch1 siRNA to SKOV3 cells by cationic cholesterol derivative-based liposome
title_fullStr Efficient delivery of Notch1 siRNA to SKOV3 cells by cationic cholesterol derivative-based liposome
title_full_unstemmed Efficient delivery of Notch1 siRNA to SKOV3 cells by cationic cholesterol derivative-based liposome
title_sort efficient delivery of notch1 sirna to skov3 cells by cationic cholesterol derivative-based liposome
publisher Dove Medical Press
publishDate 2016
url https://doaj.org/article/d111ead5713e44dcb9db23fe8276f80c
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