Cis-regulatory elements in conserved non-coding sequences of nuclear receptor genes indicate for crosstalk between endocrine systems

Nuclear receptors (NRs) are ligand-activated transcription factors that regulate gene expression when bound to specific DNA sequences. Crosstalk between steroid NR systems has been studied for understanding the development of hormone-driven cancers but not to an extent at a genetic level. This study...

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Autores principales:	Cruz Maria Araceli Diaz, Lund Dan, Szekeres Ferenc, Karlsson Sandra, Faresjö Maria, Larsson Dennis
Formato:	article
Lenguaje:	EN
Publicado:	De Gruyter 2021
Materias:	conserved sequences transcription factor binding sites splicing sites nuclear receptor binding domains crosstalk Medicine R
Acceso en línea:	https://doaj.org/article/d11528fce6834bd588bef5a0b5f6da9c
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spelling	oai:doaj.org-article:d11528fce6834bd588bef5a0b5f6da9c2021-12-05T14:10:54ZCis-regulatory elements in conserved non-coding sequences of nuclear receptor genes indicate for crosstalk between endocrine systems2391-546310.1515/med-2021-0264https://doaj.org/article/d11528fce6834bd588bef5a0b5f6da9c2021-04-01T00:00:00Zhttps://doi.org/10.1515/med-2021-0264https://doaj.org/toc/2391-5463Nuclear receptors (NRs) are ligand-activated transcription factors that regulate gene expression when bound to specific DNA sequences. Crosstalk between steroid NR systems has been studied for understanding the development of hormone-driven cancers but not to an extent at a genetic level. This study aimed to investigate crosstalk between steroid NRs in conserved intron and exon sequences, with a focus on steroid NRs involved in prostate cancer etiology. For this purpose, we evaluated conserved intron and exon sequences among all 49 members of the NR Superfamily (NRS) and their relevance as regulatory sequences and NR-binding sequences. Sequence conservation was found to be higher in the first intron (35%), when compared with downstream introns. Seventy-nine percent of the conserved regions in the NRS contained putative transcription factor binding sites (TFBS) and a large fraction of these sequences contained splicing sites (SS). Analysis of transcription factors binding to putative intronic and exonic TFBS revealed that 5 and 16%, respectively, were NRs. The present study suggests crosstalk between steroid NRs, e.g., vitamin D, estrogen, progesterone, and retinoic acid endocrine systems, through cis-regulatory elements in conserved sequences of introns and exons. This investigation gives evidence for crosstalk between steroid hormones and contributes to novel targets for steroid NR regulation.Cruz Maria Araceli DiazLund DanSzekeres FerencKarlsson SandraFaresjö MariaLarsson DennisDe Gruyterarticleconserved sequencestranscription factor binding sitessplicing sitesnuclear receptor binding domainscrosstalkMedicineRENOpen Medicine, Vol 16, Iss 1, Pp 640-650 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	conserved sequences transcription factor binding sites splicing sites nuclear receptor binding domains crosstalk Medicine R
spellingShingle	conserved sequences transcription factor binding sites splicing sites nuclear receptor binding domains crosstalk Medicine R Cruz Maria Araceli Diaz Lund Dan Szekeres Ferenc Karlsson Sandra Faresjö Maria Larsson Dennis Cis-regulatory elements in conserved non-coding sequences of nuclear receptor genes indicate for crosstalk between endocrine systems
description	Nuclear receptors (NRs) are ligand-activated transcription factors that regulate gene expression when bound to specific DNA sequences. Crosstalk between steroid NR systems has been studied for understanding the development of hormone-driven cancers but not to an extent at a genetic level. This study aimed to investigate crosstalk between steroid NRs in conserved intron and exon sequences, with a focus on steroid NRs involved in prostate cancer etiology. For this purpose, we evaluated conserved intron and exon sequences among all 49 members of the NR Superfamily (NRS) and their relevance as regulatory sequences and NR-binding sequences. Sequence conservation was found to be higher in the first intron (35%), when compared with downstream introns. Seventy-nine percent of the conserved regions in the NRS contained putative transcription factor binding sites (TFBS) and a large fraction of these sequences contained splicing sites (SS). Analysis of transcription factors binding to putative intronic and exonic TFBS revealed that 5 and 16%, respectively, were NRs. The present study suggests crosstalk between steroid NRs, e.g., vitamin D, estrogen, progesterone, and retinoic acid endocrine systems, through cis-regulatory elements in conserved sequences of introns and exons. This investigation gives evidence for crosstalk between steroid hormones and contributes to novel targets for steroid NR regulation.
format	article
author	Cruz Maria Araceli Diaz Lund Dan Szekeres Ferenc Karlsson Sandra Faresjö Maria Larsson Dennis
author_facet	Cruz Maria Araceli Diaz Lund Dan Szekeres Ferenc Karlsson Sandra Faresjö Maria Larsson Dennis
author_sort	Cruz Maria Araceli Diaz
title	Cis-regulatory elements in conserved non-coding sequences of nuclear receptor genes indicate for crosstalk between endocrine systems
title_short	Cis-regulatory elements in conserved non-coding sequences of nuclear receptor genes indicate for crosstalk between endocrine systems
title_full	Cis-regulatory elements in conserved non-coding sequences of nuclear receptor genes indicate for crosstalk between endocrine systems
title_fullStr	Cis-regulatory elements in conserved non-coding sequences of nuclear receptor genes indicate for crosstalk between endocrine systems
title_full_unstemmed	Cis-regulatory elements in conserved non-coding sequences of nuclear receptor genes indicate for crosstalk between endocrine systems
title_sort	cis-regulatory elements in conserved non-coding sequences of nuclear receptor genes indicate for crosstalk between endocrine systems
publisher	De Gruyter
publishDate	2021
url	https://doaj.org/article/d11528fce6834bd588bef5a0b5f6da9c
work_keys_str_mv	AT cruzmariaaracelidiaz cisregulatoryelementsinconservednoncodingsequencesofnuclearreceptorgenesindicateforcrosstalkbetweenendocrinesystems AT lunddan cisregulatoryelementsinconservednoncodingsequencesofnuclearreceptorgenesindicateforcrosstalkbetweenendocrinesystems AT szekeresferenc cisregulatoryelementsinconservednoncodingsequencesofnuclearreceptorgenesindicateforcrosstalkbetweenendocrinesystems AT karlssonsandra cisregulatoryelementsinconservednoncodingsequencesofnuclearreceptorgenesindicateforcrosstalkbetweenendocrinesystems AT faresjomaria cisregulatoryelementsinconservednoncodingsequencesofnuclearreceptorgenesindicateforcrosstalkbetweenendocrinesystems AT larssondennis cisregulatoryelementsinconservednoncodingsequencesofnuclearreceptorgenesindicateforcrosstalkbetweenendocrinesystems
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Cis-regulatory elements in conserved non-coding sequences of nuclear receptor genes indicate for crosstalk between endocrine systems

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