Genome-wide control of the distribution of meiotic recombination.

Meiotic recombination events are not randomly distributed in the genome but occur in specific regions called recombination hotspots. Hotspots are predicted to be preferred sites for the initiation of meiotic recombination and their positions and activities are regulated by yet-unknown controls. The...

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Autores principales:	Corinne Grey, Frédéric Baudat, Bernard de Massy
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2009
Materias:	Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/d1162f9d7d8a43af881f129d05c2d93c
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spelling	oai:doaj.org-article:d1162f9d7d8a43af881f129d05c2d93c2021-11-25T05:33:48ZGenome-wide control of the distribution of meiotic recombination.1544-91731545-788510.1371/journal.pbio.1000035https://doaj.org/article/d1162f9d7d8a43af881f129d05c2d93c2009-02-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/19226188/?tool=EBIhttps://doaj.org/toc/1544-9173https://doaj.org/toc/1545-7885Meiotic recombination events are not randomly distributed in the genome but occur in specific regions called recombination hotspots. Hotspots are predicted to be preferred sites for the initiation of meiotic recombination and their positions and activities are regulated by yet-unknown controls. The activity of the Psmb9 hotspot on mouse Chromosome 17 (Chr 17) varies according to genetic background. It is active in strains carrying a recombinant Chr 17 where the proximal third is derived from Mus musculus molossinus. We have identified the genetic locus required for Psmb9 activity, named Dsbc1 for Double-strand break control 1, and mapped this locus within a 6.7-Mb region on Chr 17. Based on cytological analysis of meiotic DNA double-strand breaks (DSB) and crossovers (COs), we show that Dsbc1 influences DSB and CO, not only at Psmb9, but in several other regions of Chr 17. We further show that CO distribution is also influenced by Dsbc1 on Chrs 15 and 18. Finally, we provide direct molecular evidence for the regulation in trans mediated by Dsbc1, by showing that it controls the CO activity at the Hlx1 hotspot on Chr 1. We thus propose that Dsbc1 encodes for a trans-acting factor involved in the specification of initiation sites of meiotic recombination genome wide in mice.Corinne GreyFrédéric BaudatBernard de MassyPublic Library of Science (PLoS)articleBiology (General)QH301-705.5ENPLoS Biology, Vol 7, Iss 2, p e35 (2009)
institution	DOAJ
collection	DOAJ
language	EN
topic	Biology (General) QH301-705.5
spellingShingle	Biology (General) QH301-705.5 Corinne Grey Frédéric Baudat Bernard de Massy Genome-wide control of the distribution of meiotic recombination.
description	Meiotic recombination events are not randomly distributed in the genome but occur in specific regions called recombination hotspots. Hotspots are predicted to be preferred sites for the initiation of meiotic recombination and their positions and activities are regulated by yet-unknown controls. The activity of the Psmb9 hotspot on mouse Chromosome 17 (Chr 17) varies according to genetic background. It is active in strains carrying a recombinant Chr 17 where the proximal third is derived from Mus musculus molossinus. We have identified the genetic locus required for Psmb9 activity, named Dsbc1 for Double-strand break control 1, and mapped this locus within a 6.7-Mb region on Chr 17. Based on cytological analysis of meiotic DNA double-strand breaks (DSB) and crossovers (COs), we show that Dsbc1 influences DSB and CO, not only at Psmb9, but in several other regions of Chr 17. We further show that CO distribution is also influenced by Dsbc1 on Chrs 15 and 18. Finally, we provide direct molecular evidence for the regulation in trans mediated by Dsbc1, by showing that it controls the CO activity at the Hlx1 hotspot on Chr 1. We thus propose that Dsbc1 encodes for a trans-acting factor involved in the specification of initiation sites of meiotic recombination genome wide in mice.
format	article
author	Corinne Grey Frédéric Baudat Bernard de Massy
author_facet	Corinne Grey Frédéric Baudat Bernard de Massy
author_sort	Corinne Grey
title	Genome-wide control of the distribution of meiotic recombination.
title_short	Genome-wide control of the distribution of meiotic recombination.
title_full	Genome-wide control of the distribution of meiotic recombination.
title_fullStr	Genome-wide control of the distribution of meiotic recombination.
title_full_unstemmed	Genome-wide control of the distribution of meiotic recombination.
title_sort	genome-wide control of the distribution of meiotic recombination.
publisher	Public Library of Science (PLoS)
publishDate	2009
url	https://doaj.org/article/d1162f9d7d8a43af881f129d05c2d93c
work_keys_str_mv	AT corinnegrey genomewidecontrolofthedistributionofmeioticrecombination AT fredericbaudat genomewidecontrolofthedistributionofmeioticrecombination AT bernarddemassy genomewidecontrolofthedistributionofmeioticrecombination
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Genome-wide control of the distribution of meiotic recombination.

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