Rootstock effects on scion gene expression in maritime pine

Abstract Pines are the dominant conifers in Mediterranean forests. As long-lived sessile organisms that seasonally have to cope with drought periods, they have developed a variety of adaptive responses. However, during last decades, highly intense and long-lasting drought events could have contribut...

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Autores principales:	M. López-Hinojosa, N. de María, M. A. Guevara, M. D. Vélez, J. A. Cabezas, L. M. Díaz, J. A. Mancha, A. Pizarro, L. F. Manjarrez, C. Collada, C. Díaz-Sala, M. T. Cervera Goy
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/d116b360253d4a49b34388e22bfbf84d
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spelling	oai:doaj.org-article:d116b360253d4a49b34388e22bfbf84d2021-12-02T15:57:11ZRootstock effects on scion gene expression in maritime pine10.1038/s41598-021-90672-y2045-2322https://doaj.org/article/d116b360253d4a49b34388e22bfbf84d2021-06-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-90672-yhttps://doaj.org/toc/2045-2322Abstract Pines are the dominant conifers in Mediterranean forests. As long-lived sessile organisms that seasonally have to cope with drought periods, they have developed a variety of adaptive responses. However, during last decades, highly intense and long-lasting drought events could have contributed to decay and mortality of the most susceptible trees. Among conifer species, Pinus pinaster Ait. shows remarkable ability to adapt to different environments. Previous molecular analysis of a full-sib family designed to study drought response led us to find active transcriptional activity of stress-responding genes even without water deprivation in tolerant genotypes. To improve our knowledge about communication between above- and below-ground organs of maritime pine, we have analyzed four graft-type constructions using two siblings as rootstocks and their progenitors, Gal 1056 and Oria 6, as scions. Transcriptomic profiles of needles from both scions were modified by the rootstock they were grafted on. However, the most significant differential gene expression was observed in drought-sensitive Gal 1056, while in drought-tolerant Oria 6, differential gene expression was very much lower. Furthermore, both scions grafted onto drought-tolerant rootstocks showed activation of genes involved in tolerance to abiotic stress, and is most remarkable in Oria 6 grafts where higher accumulation of transcripts involved in phytohormone action, transcriptional regulation, photosynthesis and signaling has been found. Additionally, processes, such as those related to secondary metabolism, were mainly associated with the scion genotype. This study provides pioneering information about rootstock effects on scion gene expression in conifers.M. López-HinojosaN. de MaríaM. A. GuevaraM. D. VélezJ. A. CabezasL. M. DíazJ. A. ManchaA. PizarroL. F. ManjarrezC. ColladaC. Díaz-SalaM. T. Cervera GoyNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-16 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q M. López-Hinojosa N. de María M. A. Guevara M. D. Vélez J. A. Cabezas L. M. Díaz J. A. Mancha A. Pizarro L. F. Manjarrez C. Collada C. Díaz-Sala M. T. Cervera Goy Rootstock effects on scion gene expression in maritime pine
description	Abstract Pines are the dominant conifers in Mediterranean forests. As long-lived sessile organisms that seasonally have to cope with drought periods, they have developed a variety of adaptive responses. However, during last decades, highly intense and long-lasting drought events could have contributed to decay and mortality of the most susceptible trees. Among conifer species, Pinus pinaster Ait. shows remarkable ability to adapt to different environments. Previous molecular analysis of a full-sib family designed to study drought response led us to find active transcriptional activity of stress-responding genes even without water deprivation in tolerant genotypes. To improve our knowledge about communication between above- and below-ground organs of maritime pine, we have analyzed four graft-type constructions using two siblings as rootstocks and their progenitors, Gal 1056 and Oria 6, as scions. Transcriptomic profiles of needles from both scions were modified by the rootstock they were grafted on. However, the most significant differential gene expression was observed in drought-sensitive Gal 1056, while in drought-tolerant Oria 6, differential gene expression was very much lower. Furthermore, both scions grafted onto drought-tolerant rootstocks showed activation of genes involved in tolerance to abiotic stress, and is most remarkable in Oria 6 grafts where higher accumulation of transcripts involved in phytohormone action, transcriptional regulation, photosynthesis and signaling has been found. Additionally, processes, such as those related to secondary metabolism, were mainly associated with the scion genotype. This study provides pioneering information about rootstock effects on scion gene expression in conifers.
format	article
author	M. López-Hinojosa N. de María M. A. Guevara M. D. Vélez J. A. Cabezas L. M. Díaz J. A. Mancha A. Pizarro L. F. Manjarrez C. Collada C. Díaz-Sala M. T. Cervera Goy
author_facet	M. López-Hinojosa N. de María M. A. Guevara M. D. Vélez J. A. Cabezas L. M. Díaz J. A. Mancha A. Pizarro L. F. Manjarrez C. Collada C. Díaz-Sala M. T. Cervera Goy
author_sort	M. López-Hinojosa
title	Rootstock effects on scion gene expression in maritime pine
title_short	Rootstock effects on scion gene expression in maritime pine
title_full	Rootstock effects on scion gene expression in maritime pine
title_fullStr	Rootstock effects on scion gene expression in maritime pine
title_full_unstemmed	Rootstock effects on scion gene expression in maritime pine
title_sort	rootstock effects on scion gene expression in maritime pine
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/d116b360253d4a49b34388e22bfbf84d
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Rootstock effects on scion gene expression in maritime pine

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