Engineered Interspecies Amino Acid Cross-Feeding Increases Population Evenness in a Synthetic Bacterial Consortium

ABSTRACT In nature, microbes interact antagonistically, neutrally, or beneficially. To shed light on the effects of positive interactions in microbial consortia, we introduced metabolic dependencies and metabolite overproduction into four bacterial species. While antagonistic interactions govern the...

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Autores principales:	Marika Ziesack, Travis Gibson, John K. W. Oliver, Andrew M. Shumaker, Bryan B. Hsu, David T. Riglar, Tobias W. Giessen, Nicholas V. DiBenedetto, Lynn Bry, Jeffrey C. Way, Pamela A. Silver, Georg K. Gerber
Formato:	article
Lenguaje:	EN
Publicado:	American Society for Microbiology 2019
Materias:	metabolite cross-feeding microbial consortia synthetic biology Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/d11d2e80b5fb41c3b5f8c22f1ff4e2ba
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spelling	oai:doaj.org-article:d11d2e80b5fb41c3b5f8c22f1ff4e2ba2021-12-02T19:47:34ZEngineered Interspecies Amino Acid Cross-Feeding Increases Population Evenness in a Synthetic Bacterial Consortium10.1128/mSystems.00352-192379-5077https://doaj.org/article/d11d2e80b5fb41c3b5f8c22f1ff4e2ba2019-08-01T00:00:00Zhttps://journals.asm.org/doi/10.1128/mSystems.00352-19https://doaj.org/toc/2379-5077ABSTRACT In nature, microbes interact antagonistically, neutrally, or beneficially. To shed light on the effects of positive interactions in microbial consortia, we introduced metabolic dependencies and metabolite overproduction into four bacterial species. While antagonistic interactions govern the wild-type consortium behavior, the genetic modifications alleviated antagonistic interactions and resulted in beneficial interactions. Engineered cross-feeding increased population evenness, a component of ecological diversity, in different environments, including in a more complex gnotobiotic mouse gut environment. Our findings suggest that metabolite cross-feeding could be used as a tool for intentionally shaping microbial consortia in complex environments. IMPORTANCE Microbial communities are ubiquitous in nature. Bacterial consortia live in and on our body and in our environment, and more recently, biotechnology is applying microbial consortia for bioproduction. As part of our body, bacterial consortia influence us in health and disease. Microbial consortium function is determined by its composition, which in turn is driven by the interactions between species. Further understanding of microbial interactions will help us in deciphering how consortia function in complex environments and may enable us to modify microbial consortia for health and environmental benefits. Author Video: An author video summary of this article is available.Marika ZiesackTravis GibsonJohn K. W. OliverAndrew M. ShumakerBryan B. HsuDavid T. RiglarTobias W. GiessenNicholas V. DiBenedettoLynn BryJeffrey C. WayPamela A. SilverGeorg K. GerberAmerican Society for Microbiologyarticlemetabolite cross-feedingmicrobial consortiasynthetic biologyMicrobiologyQR1-502ENmSystems, Vol 4, Iss 4 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	metabolite cross-feeding microbial consortia synthetic biology Microbiology QR1-502
spellingShingle	metabolite cross-feeding microbial consortia synthetic biology Microbiology QR1-502 Marika Ziesack Travis Gibson John K. W. Oliver Andrew M. Shumaker Bryan B. Hsu David T. Riglar Tobias W. Giessen Nicholas V. DiBenedetto Lynn Bry Jeffrey C. Way Pamela A. Silver Georg K. Gerber Engineered Interspecies Amino Acid Cross-Feeding Increases Population Evenness in a Synthetic Bacterial Consortium
description	ABSTRACT In nature, microbes interact antagonistically, neutrally, or beneficially. To shed light on the effects of positive interactions in microbial consortia, we introduced metabolic dependencies and metabolite overproduction into four bacterial species. While antagonistic interactions govern the wild-type consortium behavior, the genetic modifications alleviated antagonistic interactions and resulted in beneficial interactions. Engineered cross-feeding increased population evenness, a component of ecological diversity, in different environments, including in a more complex gnotobiotic mouse gut environment. Our findings suggest that metabolite cross-feeding could be used as a tool for intentionally shaping microbial consortia in complex environments. IMPORTANCE Microbial communities are ubiquitous in nature. Bacterial consortia live in and on our body and in our environment, and more recently, biotechnology is applying microbial consortia for bioproduction. As part of our body, bacterial consortia influence us in health and disease. Microbial consortium function is determined by its composition, which in turn is driven by the interactions between species. Further understanding of microbial interactions will help us in deciphering how consortia function in complex environments and may enable us to modify microbial consortia for health and environmental benefits. Author Video: An author video summary of this article is available.
format	article
author	Marika Ziesack Travis Gibson John K. W. Oliver Andrew M. Shumaker Bryan B. Hsu David T. Riglar Tobias W. Giessen Nicholas V. DiBenedetto Lynn Bry Jeffrey C. Way Pamela A. Silver Georg K. Gerber
author_facet	Marika Ziesack Travis Gibson John K. W. Oliver Andrew M. Shumaker Bryan B. Hsu David T. Riglar Tobias W. Giessen Nicholas V. DiBenedetto Lynn Bry Jeffrey C. Way Pamela A. Silver Georg K. Gerber
author_sort	Marika Ziesack
title	Engineered Interspecies Amino Acid Cross-Feeding Increases Population Evenness in a Synthetic Bacterial Consortium
title_short	Engineered Interspecies Amino Acid Cross-Feeding Increases Population Evenness in a Synthetic Bacterial Consortium
title_full	Engineered Interspecies Amino Acid Cross-Feeding Increases Population Evenness in a Synthetic Bacterial Consortium
title_fullStr	Engineered Interspecies Amino Acid Cross-Feeding Increases Population Evenness in a Synthetic Bacterial Consortium
title_full_unstemmed	Engineered Interspecies Amino Acid Cross-Feeding Increases Population Evenness in a Synthetic Bacterial Consortium
title_sort	engineered interspecies amino acid cross-feeding increases population evenness in a synthetic bacterial consortium
publisher	American Society for Microbiology
publishDate	2019
url	https://doaj.org/article/d11d2e80b5fb41c3b5f8c22f1ff4e2ba
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Engineered Interspecies Amino Acid Cross-Feeding Increases Population Evenness in a Synthetic Bacterial Consortium

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