Application of an open-chamber multi-channel microfluidic device to test chemotherapy drugs

Abstract The use of precision medicine for chemotherapy requires the individualization of the therapeutic regimen for each patient. This approach improves treatment efficacy and reduces the probability of administering ineffective drugs. To ensure accurate decision-making in a timely manner, antican...

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Autores principales:	Hui-Sung Moon, Chang Eun Yoo, Sangmin Kim, Jeong Eon Lee, Woong-Yang Park
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2020
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/d122b8b82f46452fb625391c39b13747
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spelling	oai:doaj.org-article:d122b8b82f46452fb625391c39b137472021-12-02T15:10:32ZApplication of an open-chamber multi-channel microfluidic device to test chemotherapy drugs10.1038/s41598-020-77324-32045-2322https://doaj.org/article/d122b8b82f46452fb625391c39b137472020-11-01T00:00:00Zhttps://doi.org/10.1038/s41598-020-77324-3https://doaj.org/toc/2045-2322Abstract The use of precision medicine for chemotherapy requires the individualization of the therapeutic regimen for each patient. This approach improves treatment efficacy and reduces the probability of administering ineffective drugs. To ensure accurate decision-making in a timely manner, anticancer drug efficacy tests must be performed within a short timeframe using a small number of cancer cells. These requirements can be satisfied via microfluidics-based drug screening platforms, which are composed of complex fluidic channels and closed systems. Owing to their complexity, skilled manipulation is required. In this study, we developed a microfluidic platform, to accurately perform multiple drug efficacy tests using a small number of cells, which can be conducted via simple manipulation. As it is a small, open-chamber system, a minimal number of cells could be loaded through simple pipetting. Furthermore, the extracellular matrix gel inside the chamber provides an in vivo-like environment that enables the localized delivery of the drugs to spontaneously diffuse from the channels underneath the chamber without a pump, thereby efficiently and robustly testing the efficacy and resistance of multiple drugs. We demonstrated that this platform enabled the rapid and facile testing of multiple drugs using a small number of cells (~ 10,000) over a short period of time (~ 2 days). These results provide the possibility of using this powerful platform for selecting therapeutic medication, developing new drugs, and delivering personalized medicine to patients.Hui-Sung MoonChang Eun YooSangmin KimJeong Eon LeeWoong-Yang ParkNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 10, Iss 1, Pp 1-12 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Hui-Sung Moon Chang Eun Yoo Sangmin Kim Jeong Eon Lee Woong-Yang Park Application of an open-chamber multi-channel microfluidic device to test chemotherapy drugs
description	Abstract The use of precision medicine for chemotherapy requires the individualization of the therapeutic regimen for each patient. This approach improves treatment efficacy and reduces the probability of administering ineffective drugs. To ensure accurate decision-making in a timely manner, anticancer drug efficacy tests must be performed within a short timeframe using a small number of cancer cells. These requirements can be satisfied via microfluidics-based drug screening platforms, which are composed of complex fluidic channels and closed systems. Owing to their complexity, skilled manipulation is required. In this study, we developed a microfluidic platform, to accurately perform multiple drug efficacy tests using a small number of cells, which can be conducted via simple manipulation. As it is a small, open-chamber system, a minimal number of cells could be loaded through simple pipetting. Furthermore, the extracellular matrix gel inside the chamber provides an in vivo-like environment that enables the localized delivery of the drugs to spontaneously diffuse from the channels underneath the chamber without a pump, thereby efficiently and robustly testing the efficacy and resistance of multiple drugs. We demonstrated that this platform enabled the rapid and facile testing of multiple drugs using a small number of cells (~ 10,000) over a short period of time (~ 2 days). These results provide the possibility of using this powerful platform for selecting therapeutic medication, developing new drugs, and delivering personalized medicine to patients.
format	article
author	Hui-Sung Moon Chang Eun Yoo Sangmin Kim Jeong Eon Lee Woong-Yang Park
author_facet	Hui-Sung Moon Chang Eun Yoo Sangmin Kim Jeong Eon Lee Woong-Yang Park
author_sort	Hui-Sung Moon
title	Application of an open-chamber multi-channel microfluidic device to test chemotherapy drugs
title_short	Application of an open-chamber multi-channel microfluidic device to test chemotherapy drugs
title_full	Application of an open-chamber multi-channel microfluidic device to test chemotherapy drugs
title_fullStr	Application of an open-chamber multi-channel microfluidic device to test chemotherapy drugs
title_full_unstemmed	Application of an open-chamber multi-channel microfluidic device to test chemotherapy drugs
title_sort	application of an open-chamber multi-channel microfluidic device to test chemotherapy drugs
publisher	Nature Portfolio
publishDate	2020
url	https://doaj.org/article/d122b8b82f46452fb625391c39b13747
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Application of an open-chamber multi-channel microfluidic device to test chemotherapy drugs

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