Improved thrombolytic effect with focused ultrasound and neuroprotective agent against acute carotid artery thrombosis in rat

Abstract Combination therapy with focused ultrasound (FUS) and a neuroprotective agent, BNG-1, was examined in an acute carotid thrombotic occlusion model using LED irradiation in rat to improve the thrombolytic effect of rt-PA. Seven treatment groups included (A) intravenous bolus injection of 0.45...

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Autores principales:	Tsong-Hai Lee, Jih-Chao Yeh, Chih-Hung Tsai, Jen-Tsung Yang, Shyh-Liang Lou, Chen-June Seak, Chao-Yung Wang, Kuo-Chen Wei, Hao-Li Liu
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/d12ec1f03e28435994faa940938b6daa
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spelling	oai:doaj.org-article:d12ec1f03e28435994faa940938b6daa2021-12-02T11:40:13ZImproved thrombolytic effect with focused ultrasound and neuroprotective agent against acute carotid artery thrombosis in rat10.1038/s41598-017-01769-22045-2322https://doaj.org/article/d12ec1f03e28435994faa940938b6daa2017-05-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-01769-2https://doaj.org/toc/2045-2322Abstract Combination therapy with focused ultrasound (FUS) and a neuroprotective agent, BNG-1, was examined in an acute carotid thrombotic occlusion model using LED irradiation in rat to improve the thrombolytic effect of rt-PA. Seven treatment groups included (A) intravenous bolus injection of 0.45 mg/kg rt-PA, (B) intravenous bolus injection of 0.9 mg/kg, (C) sonothrombolysis with FUS alone, (D) oral administration of 2 g/kg BNG-1 for 7 days alone, (E) A + D, (F) A + C, and (G) A + C + D. Four comparison groups were made including (H) 0.45 mg/kg rt-PA 20% bolus +80% IV fusion + FUS, (I) 0.9 mg/kg rt-PA with 10% bolus + 90% intravenous fusion, (J) B + C, (K) B + D. At 7 days after carotid occlusion, small-animal carotid ultrasound and 7 T MR angiography showed the recanalization rate of ≤50% stenosis was 50% in group B and 83% in group I, but 0% in groups A and C and 17% in group D. Combination therapy improved recanalization rate to 50–63% in groups E and F, to 67–83% in groups J and K, and to 100% in groups G and H. Our study demonstrated combination therapy with different remedies can be a feasible strategy to improve the thrombolytic effect of rt-PA.Tsong-Hai LeeJih-Chao YehChih-Hung TsaiJen-Tsung YangShyh-Liang LouChen-June SeakChao-Yung WangKuo-Chen WeiHao-Li LiuNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-11 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Tsong-Hai Lee Jih-Chao Yeh Chih-Hung Tsai Jen-Tsung Yang Shyh-Liang Lou Chen-June Seak Chao-Yung Wang Kuo-Chen Wei Hao-Li Liu Improved thrombolytic effect with focused ultrasound and neuroprotective agent against acute carotid artery thrombosis in rat
description	Abstract Combination therapy with focused ultrasound (FUS) and a neuroprotective agent, BNG-1, was examined in an acute carotid thrombotic occlusion model using LED irradiation in rat to improve the thrombolytic effect of rt-PA. Seven treatment groups included (A) intravenous bolus injection of 0.45 mg/kg rt-PA, (B) intravenous bolus injection of 0.9 mg/kg, (C) sonothrombolysis with FUS alone, (D) oral administration of 2 g/kg BNG-1 for 7 days alone, (E) A + D, (F) A + C, and (G) A + C + D. Four comparison groups were made including (H) 0.45 mg/kg rt-PA 20% bolus +80% IV fusion + FUS, (I) 0.9 mg/kg rt-PA with 10% bolus + 90% intravenous fusion, (J) B + C, (K) B + D. At 7 days after carotid occlusion, small-animal carotid ultrasound and 7 T MR angiography showed the recanalization rate of ≤50% stenosis was 50% in group B and 83% in group I, but 0% in groups A and C and 17% in group D. Combination therapy improved recanalization rate to 50–63% in groups E and F, to 67–83% in groups J and K, and to 100% in groups G and H. Our study demonstrated combination therapy with different remedies can be a feasible strategy to improve the thrombolytic effect of rt-PA.
format	article
author	Tsong-Hai Lee Jih-Chao Yeh Chih-Hung Tsai Jen-Tsung Yang Shyh-Liang Lou Chen-June Seak Chao-Yung Wang Kuo-Chen Wei Hao-Li Liu
author_facet	Tsong-Hai Lee Jih-Chao Yeh Chih-Hung Tsai Jen-Tsung Yang Shyh-Liang Lou Chen-June Seak Chao-Yung Wang Kuo-Chen Wei Hao-Li Liu
author_sort	Tsong-Hai Lee
title	Improved thrombolytic effect with focused ultrasound and neuroprotective agent against acute carotid artery thrombosis in rat
title_short	Improved thrombolytic effect with focused ultrasound and neuroprotective agent against acute carotid artery thrombosis in rat
title_full	Improved thrombolytic effect with focused ultrasound and neuroprotective agent against acute carotid artery thrombosis in rat
title_fullStr	Improved thrombolytic effect with focused ultrasound and neuroprotective agent against acute carotid artery thrombosis in rat
title_full_unstemmed	Improved thrombolytic effect with focused ultrasound and neuroprotective agent against acute carotid artery thrombosis in rat
title_sort	improved thrombolytic effect with focused ultrasound and neuroprotective agent against acute carotid artery thrombosis in rat
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/d12ec1f03e28435994faa940938b6daa
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Improved thrombolytic effect with focused ultrasound and neuroprotective agent against acute carotid artery thrombosis in rat

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