Coated electrospun alginate-containing fibers as novel delivery systems for regenerative purposes

Barbara Vigani,1 Silvia Rossi,1 Giuseppina Sandri,1 Maria Cristina Bonferoni,1 Giulia Milanesi,1 Giovanna Bruni,2 Franca Ferrari1 1Department of Drug Sciences, University of Pavia, Pavia, Italy; 2Department of Chemistry, University of Pavia, Pavia, Italy Aim: The aim of the present work was to dev...

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Autores principales:	Vigani B, Rossi S, Sandri G, Bonferoni MC, Milanesi G, Bruni G, Ferrari F
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2018
Materias:	DoE approach ALG-based fibers rheological properties conductivity surface tension chitosan PLGA Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/d13271088cf7487489f67b43b830be53
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spelling	oai:doaj.org-article:d13271088cf7487489f67b43b830be532021-12-02T04:56:00ZCoated electrospun alginate-containing fibers as novel delivery systems for regenerative purposes1178-2013https://doaj.org/article/d13271088cf7487489f67b43b830be532018-10-01T00:00:00Zhttps://www.dovepress.com/coated-electrospun-alginate-containing-fibers-as-novel-delivery-system-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Barbara Vigani,1 Silvia Rossi,1 Giuseppina Sandri,1 Maria Cristina Bonferoni,1 Giulia Milanesi,1 Giovanna Bruni,2 Franca Ferrari1 1Department of Drug Sciences, University of Pavia, Pavia, Italy; 2Department of Chemistry, University of Pavia, Pavia, Italy Aim: The aim of the present work was to develop biodegradable alginate (ALG)-containing fibrous membranes intended for tissue repair, acting as both drug delivery systems and cell growth guidance. Methods: Membranes were prepared by electrospinning. Since ALG can be electrospun only when blended with other spinnable polymers, dextran (DEX) and polyethylene oxide (PEO) were investigated as process adjuvants. ALG/DEX mixtures, characterized by different rheological and conductivity properties, were prepared in phosphate buffer or deionized water; surfactants were added to modulate polymer solution surface tension. The Design of Experiments (DoE) approach (full factorial design) was used to investigate the role of polymer solution features (rheological properties, surface tension, and conductivity) on electrospun fiber morphology. A high viscosity at 1,000 s-1 (1.3–1.9 Pa.s) or a high pseudoplasticity index (≥1.7), combined with a low surface tension (30–32 mN/m) and a low conductivity (800–1,000 µS/cm), was responsible for the production of ALG/DEX homogeneous fibers. Such ranges were successfully employed for the preparation of ALG-containing fibers, using PEO, instead of DEX, as process adjuvant. ALG/DEX and ALG/PEO fibers were subsequently subjected to cross-linking/coating processes to make them slowly biodegradable in aqueous medium. In particular, ALG/PEO fibers were cross-linked and coated with CaCl2/chitosan solutions in water/ethanol mixtures. Due to DEX high content, ALG/DEX fibers were soaked in a polylactide-co-glycolide (PLGA) solution in ethyl acetate. Results: Both cross-linking and coating processes made fibers insoluble in physiological medium and produced an increase in their mechanical resistance, assessed by means of a tensile test. PLGA-coated ALG/DEX and chitosan-coated ALG/PEO fibers were biocompatible and able to support fibroblast adhesion. Conclusion: The DoE approach allowed to draw up guidelines useful for the preparation of homogeneous fibers, starting from mixtures of ALG and non-ionic polymers. Such fibers, upon coating, resulted to be good cell substrates, allowing cell adhesion and growth. Keywords: DoE approach, ALG-based fibers, rheological properties, conductivity, surface tension, chitosan, PLGAVigani BRossi SSandri GBonferoni MCMilanesi GBruni GFerrari FDove Medical PressarticleDoE approachALG-based fibersrheological propertiesconductivitysurface tensionchitosanPLGAMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol Volume 13, Pp 6531-6550 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	DoE approach ALG-based fibers rheological properties conductivity surface tension chitosan PLGA Medicine (General) R5-920
spellingShingle	DoE approach ALG-based fibers rheological properties conductivity surface tension chitosan PLGA Medicine (General) R5-920 Vigani B Rossi S Sandri G Bonferoni MC Milanesi G Bruni G Ferrari F Coated electrospun alginate-containing fibers as novel delivery systems for regenerative purposes
description	Barbara Vigani,1 Silvia Rossi,1 Giuseppina Sandri,1 Maria Cristina Bonferoni,1 Giulia Milanesi,1 Giovanna Bruni,2 Franca Ferrari1 1Department of Drug Sciences, University of Pavia, Pavia, Italy; 2Department of Chemistry, University of Pavia, Pavia, Italy Aim: The aim of the present work was to develop biodegradable alginate (ALG)-containing fibrous membranes intended for tissue repair, acting as both drug delivery systems and cell growth guidance. Methods: Membranes were prepared by electrospinning. Since ALG can be electrospun only when blended with other spinnable polymers, dextran (DEX) and polyethylene oxide (PEO) were investigated as process adjuvants. ALG/DEX mixtures, characterized by different rheological and conductivity properties, were prepared in phosphate buffer or deionized water; surfactants were added to modulate polymer solution surface tension. The Design of Experiments (DoE) approach (full factorial design) was used to investigate the role of polymer solution features (rheological properties, surface tension, and conductivity) on electrospun fiber morphology. A high viscosity at 1,000 s-1 (1.3–1.9 Pa.s) or a high pseudoplasticity index (≥1.7), combined with a low surface tension (30–32 mN/m) and a low conductivity (800–1,000 µS/cm), was responsible for the production of ALG/DEX homogeneous fibers. Such ranges were successfully employed for the preparation of ALG-containing fibers, using PEO, instead of DEX, as process adjuvant. ALG/DEX and ALG/PEO fibers were subsequently subjected to cross-linking/coating processes to make them slowly biodegradable in aqueous medium. In particular, ALG/PEO fibers were cross-linked and coated with CaCl2/chitosan solutions in water/ethanol mixtures. Due to DEX high content, ALG/DEX fibers were soaked in a polylactide-co-glycolide (PLGA) solution in ethyl acetate. Results: Both cross-linking and coating processes made fibers insoluble in physiological medium and produced an increase in their mechanical resistance, assessed by means of a tensile test. PLGA-coated ALG/DEX and chitosan-coated ALG/PEO fibers were biocompatible and able to support fibroblast adhesion. Conclusion: The DoE approach allowed to draw up guidelines useful for the preparation of homogeneous fibers, starting from mixtures of ALG and non-ionic polymers. Such fibers, upon coating, resulted to be good cell substrates, allowing cell adhesion and growth. Keywords: DoE approach, ALG-based fibers, rheological properties, conductivity, surface tension, chitosan, PLGA
format	article
author	Vigani B Rossi S Sandri G Bonferoni MC Milanesi G Bruni G Ferrari F
author_facet	Vigani B Rossi S Sandri G Bonferoni MC Milanesi G Bruni G Ferrari F
author_sort	Vigani B
title	Coated electrospun alginate-containing fibers as novel delivery systems for regenerative purposes
title_short	Coated electrospun alginate-containing fibers as novel delivery systems for regenerative purposes
title_full	Coated electrospun alginate-containing fibers as novel delivery systems for regenerative purposes
title_fullStr	Coated electrospun alginate-containing fibers as novel delivery systems for regenerative purposes
title_full_unstemmed	Coated electrospun alginate-containing fibers as novel delivery systems for regenerative purposes
title_sort	coated electrospun alginate-containing fibers as novel delivery systems for regenerative purposes
publisher	Dove Medical Press
publishDate	2018
url	https://doaj.org/article/d13271088cf7487489f67b43b830be53
work_keys_str_mv	AT viganib coatedelectrospunalginatecontainingfibersasnoveldeliverysystemsforregenerativepurposes AT rossis coatedelectrospunalginatecontainingfibersasnoveldeliverysystemsforregenerativepurposes AT sandrig coatedelectrospunalginatecontainingfibersasnoveldeliverysystemsforregenerativepurposes AT bonferonimc coatedelectrospunalginatecontainingfibersasnoveldeliverysystemsforregenerativepurposes AT milanesig coatedelectrospunalginatecontainingfibersasnoveldeliverysystemsforregenerativepurposes AT brunig coatedelectrospunalginatecontainingfibersasnoveldeliverysystemsforregenerativepurposes AT ferrarif coatedelectrospunalginatecontainingfibersasnoveldeliverysystemsforregenerativepurposes
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Coated electrospun alginate-containing fibers as novel delivery systems for regenerative purposes

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