Investigation of Citrus HLB Symptom Variations Associated with “<i>Candidatus</i> Liberibacter asiaticus” Strains Harboring Different Phages in Southern China

Huanglongbing (HLB) is a devastating disease affecting citrus production worldwide. In China, the disease is associated with an unculturable alpha-proteobacterium, “<i>Candidatus</i> Liberibacter asiaticus” (CLas). Phages/prophages of CLas have recently been identified through intensive...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Minli Bao, Zheng Zheng, Jianchi Chen, Xiaoling Deng
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	Huanglongbing (HLB) <i>Candidatus</i> Liberibacter asiaticus phages prophages HLB symptoms graft inoculation Agriculture S
Acceso en línea:	https://doaj.org/article/d1342c3059444f9ba8af8d7727afa160
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:d1342c3059444f9ba8af8d7727afa160
record_format	dspace
spelling	oai:doaj.org-article:d1342c3059444f9ba8af8d7727afa1602021-11-25T16:09:17ZInvestigation of Citrus HLB Symptom Variations Associated with “<i>Candidatus</i> Liberibacter asiaticus” Strains Harboring Different Phages in Southern China10.3390/agronomy111122622073-4395https://doaj.org/article/d1342c3059444f9ba8af8d7727afa1602021-11-01T00:00:00Zhttps://www.mdpi.com/2073-4395/11/11/2262https://doaj.org/toc/2073-4395Huanglongbing (HLB) is a devastating disease affecting citrus production worldwide. In China, the disease is associated with an unculturable alpha-proteobacterium, “<i>Candidatus</i> Liberibacter asiaticus” (CLas). Phages/prophages of CLas have recently been identified through intensive genomic research. The phage information has facilitated research on CLas biology such as population diversity and virulence gene identification. However, little is known about the roles of CLas phages in HLB symptom development. Such research is challenging due to the unculturable nature of CLas and the lack of laboratory strains that carry a single phage. In this study, CLas strains singly carrying Type 1 phage (Type 1 CLas) and Type 2 phage (Type 2 CLas) were identified and maintained in an experimental screenhouse in southern China. The strains were characterized through next-generation sequencing (NGS). Then, each CLas strain was inoculated into seedlings of three different citrus cultivars/species through graft transmission in a screenhouse in Guangdong, China. Symptom developments were recorded. All CLas-infected cultivars showed HLB symptoms in seven months. In cultivar Nianju (<i>Citrus reticulata</i>), Type 1 CLas caused pronounced yellowing symptoms and severe defoliation, whereas Type 2 CLas caused typical Zn-deficiency-like symptoms. In contrast, symptoms from the two CLas strains’ infections on cultivars Shatianyu (<i>C. maxima</i>), and Eureka lemon (<i>Citrus limon</i>) were more difficult to differentiate. Results from this study provide baseline information for future research to investigate the roles of CLas phages in HLB symptom development.Minli BaoZheng ZhengJianchi ChenXiaoling DengMDPI AGarticleHuanglongbing (HLB)<i>Candidatus</i> Liberibacter asiaticusphagesprophagesHLB symptomsgraft inoculationAgricultureSENAgronomy, Vol 11, Iss 2262, p 2262 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Huanglongbing (HLB) <i>Candidatus</i> Liberibacter asiaticus phages prophages HLB symptoms graft inoculation Agriculture S
spellingShingle	Huanglongbing (HLB) <i>Candidatus</i> Liberibacter asiaticus phages prophages HLB symptoms graft inoculation Agriculture S Minli Bao Zheng Zheng Jianchi Chen Xiaoling Deng Investigation of Citrus HLB Symptom Variations Associated with “<i>Candidatus</i> Liberibacter asiaticus” Strains Harboring Different Phages in Southern China
description	Huanglongbing (HLB) is a devastating disease affecting citrus production worldwide. In China, the disease is associated with an unculturable alpha-proteobacterium, “<i>Candidatus</i> Liberibacter asiaticus” (CLas). Phages/prophages of CLas have recently been identified through intensive genomic research. The phage information has facilitated research on CLas biology such as population diversity and virulence gene identification. However, little is known about the roles of CLas phages in HLB symptom development. Such research is challenging due to the unculturable nature of CLas and the lack of laboratory strains that carry a single phage. In this study, CLas strains singly carrying Type 1 phage (Type 1 CLas) and Type 2 phage (Type 2 CLas) were identified and maintained in an experimental screenhouse in southern China. The strains were characterized through next-generation sequencing (NGS). Then, each CLas strain was inoculated into seedlings of three different citrus cultivars/species through graft transmission in a screenhouse in Guangdong, China. Symptom developments were recorded. All CLas-infected cultivars showed HLB symptoms in seven months. In cultivar Nianju (<i>Citrus reticulata</i>), Type 1 CLas caused pronounced yellowing symptoms and severe defoliation, whereas Type 2 CLas caused typical Zn-deficiency-like symptoms. In contrast, symptoms from the two CLas strains’ infections on cultivars Shatianyu (<i>C. maxima</i>), and Eureka lemon (<i>Citrus limon</i>) were more difficult to differentiate. Results from this study provide baseline information for future research to investigate the roles of CLas phages in HLB symptom development.
format	article
author	Minli Bao Zheng Zheng Jianchi Chen Xiaoling Deng
author_facet	Minli Bao Zheng Zheng Jianchi Chen Xiaoling Deng
author_sort	Minli Bao
title	Investigation of Citrus HLB Symptom Variations Associated with “<i>Candidatus</i> Liberibacter asiaticus” Strains Harboring Different Phages in Southern China
title_short	Investigation of Citrus HLB Symptom Variations Associated with “<i>Candidatus</i> Liberibacter asiaticus” Strains Harboring Different Phages in Southern China
title_full	Investigation of Citrus HLB Symptom Variations Associated with “<i>Candidatus</i> Liberibacter asiaticus” Strains Harboring Different Phages in Southern China
title_fullStr	Investigation of Citrus HLB Symptom Variations Associated with “<i>Candidatus</i> Liberibacter asiaticus” Strains Harboring Different Phages in Southern China
title_full_unstemmed	Investigation of Citrus HLB Symptom Variations Associated with “<i>Candidatus</i> Liberibacter asiaticus” Strains Harboring Different Phages in Southern China
title_sort	investigation of citrus hlb symptom variations associated with “<i>candidatus</i> liberibacter asiaticus” strains harboring different phages in southern china
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/d1342c3059444f9ba8af8d7727afa160
work_keys_str_mv	AT minlibao investigationofcitrushlbsymptomvariationsassociatedwithicandidatusiliberibacterasiaticusstrainsharboringdifferentphagesinsouthernchina AT zhengzheng investigationofcitrushlbsymptomvariationsassociatedwithicandidatusiliberibacterasiaticusstrainsharboringdifferentphagesinsouthernchina AT jianchichen investigationofcitrushlbsymptomvariationsassociatedwithicandidatusiliberibacterasiaticusstrainsharboringdifferentphagesinsouthernchina AT xiaolingdeng investigationofcitrushlbsymptomvariationsassociatedwithicandidatusiliberibacterasiaticusstrainsharboringdifferentphagesinsouthernchina
_version_	1718413343405899776

Investigation of Citrus HLB Symptom Variations Associated with “<i>Candidatus</i> Liberibacter asiaticus” Strains Harboring Different Phages in Southern China

Ejemplares similares