Lactobacillus helveticus SBT2171 inhibits lymphocyte proliferation by regulation of the JNK signaling pathway.

Lactobacillus helveticus SBT2171 (LH2171) is a lactic acid bacterium with high protease activity and used in starter cultures in the manufacture of cheese. We recently reported that consumption of cheese manufactured using LH2171 alleviated symptoms of dextran sodium sulfate (DSS)-induced colitis in...

Descripción completa

Guardado en:
Detalles Bibliográficos
Autores principales: Tomohiro Hosoya, Fumihiko Sakai, Maya Yamashita, Takuya Shiozaki, Tsutomu Endo, Ken Ukibe, Hiroshi Uenishi, Yukio Kadooka, Tomohiro Moriya, Hisako Nakagawa, Yosuke Nakayama, Tadaaki Miyazaki
Formato: article
Lenguaje:EN
Publicado: Public Library of Science (PLoS) 2014
Materias:
R
Q
Acceso en línea:https://doaj.org/article/d13c19d5d22748ce9f819d19c8c5de9e
Etiquetas: Agregar Etiqueta
Sin Etiquetas, Sea el primero en etiquetar este registro!
id oai:doaj.org-article:d13c19d5d22748ce9f819d19c8c5de9e
record_format dspace
spelling oai:doaj.org-article:d13c19d5d22748ce9f819d19c8c5de9e2021-11-25T05:58:31ZLactobacillus helveticus SBT2171 inhibits lymphocyte proliferation by regulation of the JNK signaling pathway.1932-620310.1371/journal.pone.0108360https://doaj.org/article/d13c19d5d22748ce9f819d19c8c5de9e2014-01-01T00:00:00Zhttps://doi.org/10.1371/journal.pone.0108360https://doaj.org/toc/1932-6203Lactobacillus helveticus SBT2171 (LH2171) is a lactic acid bacterium with high protease activity and used in starter cultures in the manufacture of cheese. We recently reported that consumption of cheese manufactured using LH2171 alleviated symptoms of dextran sodium sulfate (DSS)-induced colitis in mice. In this study, we have examined whether LH2171 itself exerts an inhibitory effect on the excessive proliferation of lymphocytes. We found that LH2171 inhibited the proliferation of LPS-stimulated mouse T and B cells, and the human lymphoma cell lines, Jurkat and BJAB. Cell cycle analysis showed an accumulation of LH2171-treated BJAB cells in the G2/M phase. Further, phosphorylation of c-Jun N-terminal kinase (JNK) and c-Jun was reduced by LH2171 in BJAB cells. Subsequently, expression of cell division cycle 2 (CDC2), regulated by the JNK signaling pathway and essential for G2/M phase progression, was inhibited by LH2171. It was also demonstrated that intraperitoneal administration of LH2171 strongly alleviated symptoms of collagen-induced arthritis (CIA) in mice. These findings suggest that LH2171 inhibits the proliferation of lymphocytes through a suppression of the JNK signaling pathway and exerts an immunosuppressive effect in vivo.Tomohiro HosoyaFumihiko SakaiMaya YamashitaTakuya ShiozakiTsutomu EndoKen UkibeHiroshi UenishiYukio KadookaTomohiro MoriyaHisako NakagawaYosuke NakayamaTadaaki MiyazakiPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 9, Iss 9, p e108360 (2014)
institution DOAJ
collection DOAJ
language EN
topic Medicine
R
Science
Q
spellingShingle Medicine
R
Science
Q
Tomohiro Hosoya
Fumihiko Sakai
Maya Yamashita
Takuya Shiozaki
Tsutomu Endo
Ken Ukibe
Hiroshi Uenishi
Yukio Kadooka
Tomohiro Moriya
Hisako Nakagawa
Yosuke Nakayama
Tadaaki Miyazaki
Lactobacillus helveticus SBT2171 inhibits lymphocyte proliferation by regulation of the JNK signaling pathway.
description Lactobacillus helveticus SBT2171 (LH2171) is a lactic acid bacterium with high protease activity and used in starter cultures in the manufacture of cheese. We recently reported that consumption of cheese manufactured using LH2171 alleviated symptoms of dextran sodium sulfate (DSS)-induced colitis in mice. In this study, we have examined whether LH2171 itself exerts an inhibitory effect on the excessive proliferation of lymphocytes. We found that LH2171 inhibited the proliferation of LPS-stimulated mouse T and B cells, and the human lymphoma cell lines, Jurkat and BJAB. Cell cycle analysis showed an accumulation of LH2171-treated BJAB cells in the G2/M phase. Further, phosphorylation of c-Jun N-terminal kinase (JNK) and c-Jun was reduced by LH2171 in BJAB cells. Subsequently, expression of cell division cycle 2 (CDC2), regulated by the JNK signaling pathway and essential for G2/M phase progression, was inhibited by LH2171. It was also demonstrated that intraperitoneal administration of LH2171 strongly alleviated symptoms of collagen-induced arthritis (CIA) in mice. These findings suggest that LH2171 inhibits the proliferation of lymphocytes through a suppression of the JNK signaling pathway and exerts an immunosuppressive effect in vivo.
format article
author Tomohiro Hosoya
Fumihiko Sakai
Maya Yamashita
Takuya Shiozaki
Tsutomu Endo
Ken Ukibe
Hiroshi Uenishi
Yukio Kadooka
Tomohiro Moriya
Hisako Nakagawa
Yosuke Nakayama
Tadaaki Miyazaki
author_facet Tomohiro Hosoya
Fumihiko Sakai
Maya Yamashita
Takuya Shiozaki
Tsutomu Endo
Ken Ukibe
Hiroshi Uenishi
Yukio Kadooka
Tomohiro Moriya
Hisako Nakagawa
Yosuke Nakayama
Tadaaki Miyazaki
author_sort Tomohiro Hosoya
title Lactobacillus helveticus SBT2171 inhibits lymphocyte proliferation by regulation of the JNK signaling pathway.
title_short Lactobacillus helveticus SBT2171 inhibits lymphocyte proliferation by regulation of the JNK signaling pathway.
title_full Lactobacillus helveticus SBT2171 inhibits lymphocyte proliferation by regulation of the JNK signaling pathway.
title_fullStr Lactobacillus helveticus SBT2171 inhibits lymphocyte proliferation by regulation of the JNK signaling pathway.
title_full_unstemmed Lactobacillus helveticus SBT2171 inhibits lymphocyte proliferation by regulation of the JNK signaling pathway.
title_sort lactobacillus helveticus sbt2171 inhibits lymphocyte proliferation by regulation of the jnk signaling pathway.
publisher Public Library of Science (PLoS)
publishDate 2014
url https://doaj.org/article/d13c19d5d22748ce9f819d19c8c5de9e
work_keys_str_mv AT tomohirohosoya lactobacillushelveticussbt2171inhibitslymphocyteproliferationbyregulationofthejnksignalingpathway
AT fumihikosakai lactobacillushelveticussbt2171inhibitslymphocyteproliferationbyregulationofthejnksignalingpathway
AT mayayamashita lactobacillushelveticussbt2171inhibitslymphocyteproliferationbyregulationofthejnksignalingpathway
AT takuyashiozaki lactobacillushelveticussbt2171inhibitslymphocyteproliferationbyregulationofthejnksignalingpathway
AT tsutomuendo lactobacillushelveticussbt2171inhibitslymphocyteproliferationbyregulationofthejnksignalingpathway
AT kenukibe lactobacillushelveticussbt2171inhibitslymphocyteproliferationbyregulationofthejnksignalingpathway
AT hiroshiuenishi lactobacillushelveticussbt2171inhibitslymphocyteproliferationbyregulationofthejnksignalingpathway
AT yukiokadooka lactobacillushelveticussbt2171inhibitslymphocyteproliferationbyregulationofthejnksignalingpathway
AT tomohiromoriya lactobacillushelveticussbt2171inhibitslymphocyteproliferationbyregulationofthejnksignalingpathway
AT hisakonakagawa lactobacillushelveticussbt2171inhibitslymphocyteproliferationbyregulationofthejnksignalingpathway
AT yosukenakayama lactobacillushelveticussbt2171inhibitslymphocyteproliferationbyregulationofthejnksignalingpathway
AT tadaakimiyazaki lactobacillushelveticussbt2171inhibitslymphocyteproliferationbyregulationofthejnksignalingpathway
_version_ 1718414369550761984